[1]胡澜怀,周 霞.高原地区流感病毒培养的条件优化[J].现代检验医学杂志,2016,31(03):131-134.[doi:10.3969/j.issn.1671-7414.2016.03.037]
　HU Lan-huai,ZHOU Xia.Exploration of the Most Suitable Condition for Culturing Influenza Virus on Plateau Region[J].Journal of Modern Laboratory Medicine,2016,31(03):131-134.[doi:10.3969/j.issn.1671-7414.2016.03.037]
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高原地区流感病毒培养的条件优化()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
131-134
栏目:
研究简报·实验技术
出版日期:
2016-06-15

文章信息/Info

Title:
Exploration of the Most Suitable Condition for Culturing Influenza Virus on Plateau Region
作者:
胡澜怀; 周 霞
攀枝花市疾病预防控制中心,四川攀枝花 617000
Author(s):
HU Lan-huai; ZHOU Xia
Panzhihua Center for Disease Control and Prevention,Sichuan Panzhihua 617000,China
关键词:
流感病毒;  犬肾细胞;  培养条件
分类号:
R373.13; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.03.037
文献标志码:
A
摘要:
目的 探索高原地区流感病毒在犬肾细胞(MDCK)上的最佳培养条件,提高流感病毒的分离效果。方法 将原始标本分别接种于MDCK细胞,通过比较CO2百分浓度(5%,4.8%,4.6%,4.4%,4.2%),TPCK-胰酶浓度(1,2,3,4,5,6,8,10 μg/ml),接种量(100,200,300,400,500 μl),培养时间(48,72,96,120,144 h),接种方法(吸附接种,直接接种),培养容器(细胞瓶,6孔板)对流感病毒的影响,确定最佳培养条件,对分离的各型病毒用红细胞凝集(HA)检测病毒滴度。结果 通过病毒血凝素滴度结果比较,4.4% CO2与4.6% CO2,2μg/ml TPCK-胰酶,300 μl与400 μl的接种量、吸附接种、72 h～96 h的培养时间、6孔板收获的病毒血凝素滴度高。结论 在高原地区实验室建立了MDCK细胞培养流感病毒的最优条件。
Abstract:
Objective To explore the most suitable condition of influenza virus which was culturing on plateau region,and improve the effect of the separation of influenza virus.Methods The original specimens were respective inoculated in MDCK cells,by comparing CO2 percent concentration(5%,4.8%,4.6%,4.4%,4.2%),TPCK-pancreatic enzyme dosage(1,2,3,4,5μg/m and 6,8,10 μg/ml),inoculation amount(100,200,300,400,500 μl),incubation time(48,72,96,120,144 h),inoculation methods(adsorption,direct inoculated),and culture vessel(cells,6 orifice)that wereinfluenced on influenza virus,the best culture conditions was determined.Hemagglutinatio(HA)method was used to detect the virus titers.ResultsThrough the comparison,the HA titer of virus was in the highest titer with 6 orifice plate culture vessel,4.4% CO2 and 4.6% CO2,2μg/ml TPCK-pancreatic enzyme,300 μl and 400 μl inoculation amount,72h～96h of incubation time.Conclusion The optimal condition of the influenza virus cultured with the MDCK cell has been established in thelaboratory on plateau region.

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备注/Memo

备注/Memo:
作者简介:胡澜怀(1962-),男,副主任技师,研究方向:微生物检验与质量控制。
更新日期/Last Update: 2016-06-25