[1]李腾达,刘挺挺,吴林洪,等.原发性胆汁性肝硬化患者外周血单个核细胞中Tim-1表达增高及其意义[J].现代检验医学杂志,2016,31(04):41-43.[doi:10.3969/j.issn.16717-414.2016.04.010]
　LI Teng-da,LIU Ting-ting,WU Lin-hong,et al.Increasing Expression of Tim-1 in Peripheral Blood Mononuclear Cell of Patients with Primary Biliary Cirrhosis and Its Significance[J].Journal of Modern Laboratory Medicine,2016,31(04):41-43.[doi:10.3969/j.issn.16717-414.2016.04.010]
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原发性胆汁性肝硬化患者外周血单个核细胞中Tim-1表达增高及其意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年04期
页码:
41-43
栏目:
论著
出版日期:
2016-08-10

文章信息/Info

Title:
Increasing Expression of Tim-1 in Peripheral Blood Mononuclear Cell of Patients with Primary Biliary Cirrhosis and Its Significance
文章编号:
1671-7414(2016)04-041-03
作者:
李腾达; 刘挺挺; 吴林洪; 邓顺江; 陈 燕; 谷明莉; 邓安梅
第二军医大学长海医院实验诊断科,上海 200433
Author(s):
LI Teng-da; LIU Ting-ting; WU Lin-hong; DENG Shun-jiang; CHEN Yan; GU Ming-li; DENG An-mei
Laboratory Diagnostics Division, Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China
关键词:
原发性胆汁性肝硬化;  T细胞免疫球蛋白黏蛋白分子-1;  免疫功能;  干扰素-γ;  白介素-4
分类号:
R575.22; R392.11
DOI:
10.3969/j.issn.16717-414.2016.04.010
文献标志码:
A
摘要:
目的 检测原发性胆汁性肝硬化(PBC)患者外周血Tim-1,IFN-γ和IL-4的表达水平,分析其相关性,初步探讨Tim-1在PBC诊治中的临床意义。方法 收集2013年6月～2015年11月来长海医院就诊的32例PBC患者和32例同期就诊的健康成人的外周血,分离外周血单个核细胞(PBMC),反转录酶-聚合酶链锁反应(RT-PCR)法检测PBMC中Tim-1 mRNA,酶联免疫吸附试验(ELISA)法检测血浆中Tim-1蛋白表达量,对Tim-1和IFN-γ,IL-4之间的关系进行Pearson相关性分析。结果患者组和对照组Tim-1 mRNA分别为0.63±0.40和0.36±0.32,差异具有统计学意义(t=3.005,P=0.003,P<0.01)。患者组与对照组Tim-1蛋白表达水平分别为62.72±38.60和35.66±30.55 pg/ml(t=3.11,P=0.002 8),IFN-γ表达水平分别为200.81±18.06和79.23±20.02 pg/ml(t=25.51,P<0.000 1),IL-4表达水平分别为99.22±14.67和30.18±20.56 pg/ml,差异均具有统计学意义(t=15.46,P<0.000 1)。Pearson相关分析结果提示Tim-1与IFN-γ呈负相关(r=-0.552,P=0.001 1),与IL-4呈正相关(r=0.652,P<0.000 1)。结论Tim-1可能参与了PBC的发病机制,是该病潜在的标志物和治疗靶点。
Abstract:
Objective To test the expression level of Tim-1,IFN-γ,IL-4 in peripheral blood of patients with primary biliary cirrhosis,analyze their correlation and preliminarily explore the clinical significance of Tim-1.Methods The peripheral blood were collectedfrom 32 patients with PBC in June,2013 to December,2015 and 32 health individuals at the same time.PBMC was separated from the peripheral blood,mRNA expression of Tim-1 in PBMC was measured by reverse transcriptase-polymerase chain reaction(RT-PCR).Tim-1 protein existed in plasma was tested by enzyme-linkedimmunosorbent assay(ELISA).Pearson correlation analysis could be done to determine the relationship between Tim-1 and IFN-γ,IL-4.Results The mRNA level of Tim-1 in PBMC of patient and control groups were 0.63±0.40,0.36±0.32 separately,(t=3.005,P=0.003)with significant statistical difference(P<0.01).Expression levels of Tim-1 protein in patient and control groups were 62.72±38.60,35.66±30.55 pg/ml separately(t=3.11,P=0.002 8),expression levels of IFN-γ were 200.81±18.06,79.23±20.02 pg/ml separately(t=25.51,P<0.000 1),that of IL-4 were 99.22±14.67,30.18±20.56 pg/ml separately(t=15.46,P<0.000 1),the differences were all with statistical meaning.The results of pearson correlation analysis suggested that Tim-1 in plasma was negatively related with IFN-γ(r=-0.552,P=0.001 1)and positively with IL-4(r=0.652,P<0.000 1).Conclusion Tim-1 may be involved in pathogenesis of PBC,itcan be the potential biomarker and therapeutic target of this disease.

参考文献/References:

[1] Carey EJ,Ali AH,Lindor KD.Primary biliary cirrhosis[J].Lancet,2015,386(10003):1565-1575.
[2] Fenoglio D,Bernuzzi F,Battaglia F,et al.Th17 and regulatory T lymphocytes in primary biliary cirrhosis and systemic sclerosis as models of autoimmunefibrotic diseases[J].Autoimmunity Reviews,2012,12(2):300-304.
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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教委基金(D14017),军队科研基金(BWS14J023,12MA056,15ZD009),美捷登基金(MJR20150019)。
作者简介:李腾达(1990-),女,在读硕士研究生,主要从事感染和自身免疫研究,Tel:18302192
更新日期/Last Update: 2016-08-10