[1]李明安,司丽媛,吴红波,等.哮喘患儿外周血单个核细胞瘦素及Foxp3的表达[J].现代检验医学杂志,2016,31(04):58-61,64.[doi:10.3969/j.issn.16717-414.2016.04.015]
　LI Ming-an,SI Li-yuan,WU Hong-bo,et al.Expression of Leptin and Foxp3 in Peripheral Blood Mononuclear Cells in Children with Bronchial Asthma[J].Journal of Modern Laboratory Medicine,2016,31(04):58-61,64.[doi:10.3969/j.issn.16717-414.2016.04.015]
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哮喘患儿外周血单个核细胞瘦素及Foxp3的表达()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年04期
页码:
58-61,64
栏目:
论著
出版日期:
2016-08-10

文章信息/Info

Title:
Expression of Leptin and Foxp3 in Peripheral Blood Mononuclear Cells in Children with Bronchial Asthma
文章编号:
1671-7414(2016)04-058-05
作者:
李明安1; 2; 司丽媛2; 吴红波2; 马 萍1
1.徐州医学院医学技术学院,江苏徐州 221004;
2.沭阳县人民医院,江苏沭阳 223600
Author(s):
LI Ming-an1; 2; SI Li-yuan2; WU Hong-bo2; MA Ping1
1.Department of Medical Technology,Xuzhou Medical College,Jiangsu Xuzhou 221004,China;
2.Shuyang People's Hospital,Jiangsu Shuyang 223600,China
关键词:
瘦素;  哮喘;  外周血单个核细胞;  叉头状转录因子P3;  CD4+CD25+调节性T细胞
分类号:
R562.25; R392.11
DOI:
10.3969/j.issn.16717-414.2016.04.015
文献标志码:
A
摘要:
目的 探讨哮喘患儿瘦素及CD4+CD25+调节性T细胞(Treg)特异性转录因子叉头状转录因子P3(Foxp3)在外周血单个核细胞(PBMC)中的表达及意义。方法 选取2013年8月～2015年5月在沭阳县人民医院门诊及住院的28例哮喘患儿发作期(发作组),26例哮喘患儿缓解期(缓解组)及25例健康体检儿童(对照组),采用ELISA法检测各组血浆及PBMC培养上清液中的瘦素浓度,实时荧光定量PCR法测定PBMC中Foxp3的相对表达量,进行统计学分析。结果 发作组、缓解组和对照组三组血浆浓度(19.98±5.40 ng/ml,13.73±2.28 ng/ml,12.17±3.95 ng/ml)及PBMC培养上清液瘦素浓度(55.94±11.09 pg/ml,31.97±7.71 pg/ml,27.85±7.67 pg/ml)差异均有统计学意义(F=27.07,75.96,P均<0.01); 组间两两比较,发作组均高于缓解组与对照组(P均<0.01),而缓解组与对照组差异无统计学意义(P>0.05)。发作组、缓解组和对照组三组PBMC Foxp3相对表达量(2.70±0.48,3.84±0.45,3.77±0.38)差异有统计学意义(F=57.35,P<0.01); 组间两两比较,发作组低于缓解组与对照组(P<0.01),而缓解组与对照组差异无统计学意义(P>0.05)。发作组患儿PBMC培养上清液瘦素浓度与PBMC Foxp3相对表达量呈负相关(R=-0.730,P<0.01),而血浆瘦素浓度与PBMC Foxp3相对表达量无明显相关性(R=-0.367,P=0.550)。结论 哮喘患儿PBMC分泌瘦素增加,且分泌的瘦素抑制了Foxp3的表达。
Abstract:
Objective To investigate the expression and significanc of the leptin derived by the peripheral blood mononuclear cells(PBMC)and forkhead transcription factor3(Foxp3,the transcription factor of CD4+CD25+Treg)in children with asthma.Methods Levels of plasma leptin and PBMC-derived leptin were measured by ELISA,and real-time fluorescence quantitative PCR was used to detect the expression of relative levels of Foxp3 in PBMC in 79 children,including 28 children of acute attack group,26 children of remission group,25 children of healthy control group and then statistical analysis was carried out.Results It was showed that levels of plasma leptin(19.98±5.40 ng/ml,13.73±2.28 ng/ml,12.17±3.95 ng/ml,F=27.07,all P<0.01)and PBMC-derived leptin(55.94±11.09 pg/ml,31.97±7.71 pg/ml,27.85±7.67 pg/ml,F=75.96,all P<0.01)was higher in attack stage than in remission stage and in control group,in contrast,Foxp3 mRNA in asthmatic children was lower in attack stage than in remissionstage and control group(2.70±0.48,3.84±0.45,3.77±0.38,F=57.35,P<0.01),However,there was no significant difference between asthmatic children in remission stage and healthy group(all P>0.05).Furthermore,the results of this study showed that PBMC-derived leptin positively correlated to Foxp3 mRNA(R<-0.730; P<0.01),but plasma leptin and Foxp3 mRNA had no obvious correlation(R=-0.367; P=0.550)in asthmatic children in attack stage.Conclusion The leptin derived by the PBMC was increased in children with asthma in attack stage and these leptin inhibits the expressionof Foxp3.

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备注/Memo

备注/Memo:

作者简介:李明安(1982-),男,本科,主管技师,主要从事临床免疫学检验与研究,E-mail:adimeix@163.com。
通讯作者:马 萍,主任技师,教授,硕士研究生导师,E-mail:pingm62@aliyun.com。
更新日期/Last Update: 2016-08-10