[1]秦 琴,王 慧,庄绪冉,等.慢性感染临床微生物样本中持留菌的特性研究[J].现代检验医学杂志,2016,31(05):5-8.[doi:10.3969/j.issn.1671-7414.2016.05.002]
　QIN Qin,WANG Hui,ZHUANG Xu-ran,et al.Characteristics of Persisters Isolated from Chronic Infected Patients[J].Journal of Modern Laboratory Medicine,2016,31(05):5-8.[doi:10.3969/j.issn.1671-7414.2016.05.002]
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慢性感染临床微生物样本中持留菌的特性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
5-8
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Characteristics of Persisters Isolated from Chronic Infected Patients
文章编号:
1671-7414(2016)05-005-04
作者:
秦 琴; 王 慧; 庄绪冉; 吴林洪; 李 虎; 王 靖; 邓安梅
第二军医大学长海医院实验诊断科,上海 200433
Author(s):
QIN Qin; WANG Hui; ZHUANG Xu-ran; WU Lin-hong; LI Hu; WANG Jing; DENG An-mei
Department of Clinical Laboratory Diagnostics, Changhai Hospital,Second Military Medical University,Shanghai 200433,China
关键词:
持留菌;  抗生素种类;  抗生素浓度;  感染时间
分类号:
R446.5
DOI:
10.3969/j.issn.1671-7414.2016.05.002
文献标志码:
A
摘要:
目的 了解慢性感染患者微生物样本中持留菌的持留特性,明确持留菌与感染时间及抗生素之间的关系。方法 将41株持留菌分离株(4株大肠埃希菌,23株肺炎克雷伯菌,8株铜绿假单胞菌,6株金黄色葡萄球菌)置于不同抗生素种类和浓度(10×MIC,50×MIC和100×MIC)以及营养不足的生长条件下,通过其在各环境中的特异性生长曲线分析其持留水平。结果 随着抗生素浓度增加,持留菌比例逐渐下降,抗生素浓度到达100MIC其本可以将持留菌全部清除; 同一株持留菌对不同抗生素的持留水平不同,其中大肠埃希菌对头孢噻肟的持留水平最低,肺炎克雷伯菌对环丙沙星的持留水平最低,铜绿假单胞菌对左氧氟沙星的持留水平最低,金黄色葡萄球菌对亚胺培南的持留水平最低; 持留菌对于营养不足的生长条件耐受明显要高于对照株(存活率10-3:10-6,P<0.05); 用独立样本的单因素方差分析同一患者不同感染时期持留菌的持留水平差异没有统计学意义(P>0.05)。结论 持留株对不同种类和浓度的抗生素具有不同的耐受能力。这为深入了解持留菌与慢性感染的关系、持留菌在临床中的不同持留特性提供了重要信息。
Abstract:
Objective To investigate the characteristics ofpersisters isolated from the chronic infected patients and clarify the relationship between the infection time and persistence as well as antibiotic drugs,soas to provide scientific basis for clinical effective measures to prevent,control and treatment of persister-associated chronic infection.MethodsThe tolerant ability of the 41 persisters(4 strains of Escherichia coli,23 strains of Klebsiella pneumoniae,8 strains of Pseudomonas aeruginosa and 6 strains of Staphylococcus aureus)under differet kind andconcentration of antibiotics and nutritional deficiencies cinditions were detected.In addition,analysis of the homologous strains isolated from the same patients with different infection time was performed.Results The study found that with the increase of concentration of antibiotics,the proportion of persisters would gradually decrease.The tolerance ability of the samepersister strain to different antibiotics was dissimilar,and different speciespersisters also had various tolerance ability to the same kind of antibiotics.Persisters were more able to withstand the lack of nutrition.What's more,there is no significant difference between the resistant ability of the homologous strains isolated from the same patients with different infection time(P>0.05).Conclusion Persisters have different ability to stand the kill of different kinds and concentration of antibiotics and they can evenbe completely removed when exposed to sufficiently high concentrations of antibiotic.

参考文献/References:

[1] Lewis K.Persister cells[J].Annu Rev Microbiol,201 0,64(1):357-372.
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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81202353,81273282,81300748),军队科研基金(BWS14J023),长海医院学科特色培育基金(CH125530300)。
作者简介:秦 琴(1978-),女,硕士,主治医师,研究方向为临床微生物学及细菌耐药机制研究,E-mail:qinq78@163.com。
通讯作者:邓安梅,主任医师,Tel:021-31162061,E-mail:amdeng70@163.com。
更新日期/Last Update: 2016-10-30