[1]万淑君,王 成,王 静,等.2型糖尿病微血管并发症患者血清miR-16,miR-126和miR-221水平检测及临床意义[J].现代检验医学杂志,2016,31(05):9-13.[doi:10.3969/j.issn.1671-7414.2016.05.003]
　WAN Shu-jun,WANG Cheng,WANG Jing,et al.Study on Serum Levels of miR-16,miR-126 and miR-221 and Their Clinical Significance in Type 2 Diabetes Patients with or Without Microvascular Complications[J].Journal of Modern Laboratory Medicine,2016,31(05):9-13.[doi:10.3969/j.issn.1671-7414.2016.05.003]
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2型糖尿病微血管并发症患者血清miR-16,miR-126和miR-221水平检测及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
9-13
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Study on Serum Levels of miR-16,miR-126 and miR-221 and Their Clinical Significance in Type 2 Diabetes Patients with or Without Microvascular Complications
文章编号:
1671-7414(2016)05-009-05
作者:
万淑君1; 2; 王 成2; 王 静2; 牛冬梅2; 张春妮2; 汪俊军2
1.江苏大学临床医学院, 江苏镇江 212013;
2.中国人民解放军南京军区南京总医院临床检验科,南京 210002
Author(s):
WAN Shu-jun1; 2; WANG Cheng2; WANG Jing2; NIU Dong-mei2; ZHANG Chun-ni2; WANG Jun-jun2
1.Jiangsu University School of Medicine,Jiangsu Zhenjiang 212013,China;
2.Department of Clinical Laboratory, Nanjing General Hospital of Nanjing Military Commend,PLA,Nanjing 210002,China
关键词:
2型糖尿病;  微血管性并发症;  微小核糖核酸;  血清;  生物标志物
分类号:
R587.2; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.05.003
文献标志码:
A
摘要:
目的 检测2型糖尿病(T2DM)及其微血管并发症患者(T2DMC)血清中miR-16,miR-126和miR-221水平,分析其作为T2DMC潜在辅助诊断指标的潜能。方法 运用实时荧光定量PCR技术(qRT-PCR)检测55例T2DM患者,55例T2DMC患者及55例健康对照血清中miR-16,miR-126和miR-221水平,生化分析仪测定相关生化指标,ROC曲线及逻辑回归分析三种血清miRNAs对T2DM及T2DMC的辅助诊断价值。结果 qRT-PCR 结果显示,与健康对照组[miR-16(14.35±1.00)×10-5,miR-126(11.75±1.47)×10-5和miR-221(32.26±3.98)×10-5]相比,三种miRNAs在T2DM患者[miR-16(23.74±2.70)×10-5,miR-126(25.01±4.13)×10-5和miR-221(84.76±11.79)×10-5]及T2DMC患者[miR-16(43.74±9.61)×10-5,miR-126(17.66±2.20)×10-5和miR-221(82.52±12.48)×10-5]血清中表达水平显著升高(P均<0.05),其中miR-16变化最为显著; 三种miRNAs用于T2DM诊断的ROC曲线下面积(AUCROC)分别为0.63(95%CI 0.53～0.74),0.64(95%CI 0.54～0.74)和0.74(95%CI 0.65～0.83); 用于T2DMC诊断的ROC曲线下面积(AUCROC)分别为0.75(95%CI 0.66～0.84),0.62(95%CI 0.52～0.73)和0.73(95%CI 0.64～0.83); 进一步逻辑回归分析结果显示,这三种血清miRNAs是T2DM和T2DMC独立危险因素。结论 血清中高表达的miR-16,miR-126和miR-221是T2DM及T2DMC患者潜在的非侵入性辅助诊断标志物及独立危险因素。
Abstract:
Objective To determine the serum levels of miR-16,miR-126 and miR-221 in type 2 diabetes(T2DM)patients with or without microvascular complications,and further evaluate their clinical significance.Methods The serum levels of miR-16,miR-126 and miR-221were examined in 55 T2DM patients,55 T2DM patients with microvascular complications and 55 healthy controls using quantitative real-time PCR(qRT-PCR).The levels of fasting blood glucose,triglycerides,cholesterol,high density lipoprotein,low density lipoprotein and others biochemical parameters were determinedby biochemical analyzer,and the diagnostic usefulness of the three miRNAs for T2DM patients and patients with microvascular complications were assessed by ROCcurve analysis and logistic regression analysis.Results Compared with healthy controls [miR-16(14.35±1.00)×10-5,miR-126(11.75±1.47)×10-5 and miR-221(32.26±3.98)×10-5],the miR-16,miR-126 and miR-221 expression were significantly increased in T2DM patients [miR-16(23.74±2.70)×10-5,miR-126(25.01±4.13)×10-5 and miR-221(84.76±11.79)×10-5] and T2DM patients with microvascular complications [miR-16(43.74±9.61)×10-5,miR-126(17.66±2.20)×10-5 and miR-221(82.52±12.48)×10-5].The area under ROC curve(AUCROC)of miR-16,miR-126 and miR-221 for T2DM patients were 0.63(95%CI 0.53～0.74),0.64(95%CI 0.54～0.74)and 0.74(95%CI 0.65～0.83),respectively.For T2DM patients with microvascular complications,the area under ROC curve were 0.75(95%CI 0.66～0.84),0.62(95%CI 0.52～0.73)and 0.73(95%CI 0.64～0.83),respectively.Furthermore,logistic regression revealed that the three miRNAs were novel independent risk factors for T2DM and T2DMC.Conclusion The levels of miR-16,miR-126 and miR-221 were significantly increased in the serum of T2DM patients with or without microvascular complications,and can be used as potential non-invasive biomarkers and risk factorsfor T2DM patients and T2DM patients with microvascular complications.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(812711904,81572074); 江苏省自然科学基金(BK20140730)。
作者简介:万淑君(1990-),女,医学硕士研究生,主要研究方向:miRNAs与糖尿病微血管病变,E-mail:834555205@qq.com。
通讯作者:汪俊军(1966-),男,博士,主任技师,教授,E-mail:wangjj9202@163.com。
更新日期/Last Update: 2016-10-30