[1]杨 铭,林 芳,和 婷,等.Stathmin表达水平与乳腺癌细胞侵袭能力相关性研究[J].现代检验医学杂志,2016,31(05):14-17.[doi:10.3969/j.issn.1671-7414.2016.05.004]
　YANG Ming,LIN Fang,HE Ting,et al.Correlation of Breast Cancer Cells Invasive and Stathmin Gene Expression[J].Journal of Modern Laboratory Medicine,2016,31(05):14-17.[doi:10.3969/j.issn.1671-7414.2016.05.004]
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Stathmin表达水平与乳腺癌细胞侵袭能力相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
14-17
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Correlation of Breast Cancer Cells Invasive and Stathmin Gene Expression
文章编号:
1671-7414(2016)05-014-04
作者:
杨 铭; 林 芳; 和 婷; 王 琳; 董 轲; 张惠中
第四军医大学唐都医院临床实验与检验科,西安 710038
Author(s):
YANG Ming; LIN Fang; HE Ting; WANG Lin; DONG Ke; ZHANGHui-zhong
Department of Clinical Laboratory, Tangdu Hospital of the Fourth Military Medical University,Xi'an 710038,China
关键词:
Stathmin;  乳腺癌;  侵袭性
分类号:
R737.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.05.004
文献标志码:
A
摘要:
目的 探讨乳腺癌细胞MDA-MB-231和MCF-7中Stathmin基因表达水平与细胞生长、黏附、侵袭等生物学行为之间的关系,为进一步研究乳腺癌转移机制奠定实验基础。方法 应用RT-PCR和Western Blot方法检测MDA-MB-231和MCF-7细胞中Stathmin基因的表达水平,同时利用细胞增殖试验、细胞黏附试验和细胞侵袭试验检测MDA-MB-231和MCF-7细胞的生长、黏附、侵袭能力,分析Stathmin表达与细胞的生长、黏附、侵袭能力之间的关系。结果 RT-PCR和Western Blot检测结果显示,Stathmin基因在MDA-MB-231和MCF-7细胞中表达均高于正常对照细胞(F=10.173,P<0.05),且MDA-MB-231细胞中的表达水平明显高于MCF-7细胞中的表达水平(t=4.562,P<0.05)。而MDA-MB-231细胞在生长、黏附和侵袭能力方面均强于MCF-7细胞(P<0.05)。结论 Stathmin表达水平高的乳腺癌细胞相应的生长、黏附、侵袭能力较强,Stathmin表达水平与细胞侵袭能力密切相关。
Abstract:
Objective To explore the relationship betweenthe Stathmin gene expression in breast cancer cells MDA-MB-231,MCF-7 and the biological behaviours such as cell growth,adhesion and invasion,and provideexperimental basis of breast cancer metastasis for further study.Methods Used RT-PCR and Western Blot methods to detect the Stathmingene expression levels in MDA-MB-231 and MCF-7 cells,and in the mean while to test the MDA-MB -231 and MCF-7 cell growth,adhesion,invasion ability by CCK-8 cell proliferation experiments,cell adhesion experiments,cell invasion experiments,then,analyed the relationship of Stathmin gene expression and cellgrowth,adhesion,invasion ability.Results Over-expression levels of Stathmin gene were observed both in the MDA-MB-231 and MCF-7 cells(F=10.173,P<0.05),and furthermore,the expression levels of Stathmin gene in MDA-MB-231 cells was higher than in MCF-7 cells(t=4.562,P<0.05).While,the growth,adhesion and invasion ability of the MDA-MB-231 cells was higher than that of MCF-7 cells(P<0.05).Conclusion The higher level of Stathmin gene expression,the stronger breast cancercells had ability of growth,invasion,and adhesive.The Stathmin gene expression levels was closely correlated with breast cancer cell invasive.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81372836)。
作者简介:杨 铭(1985-),女,硕士研究生,研究方向:肿瘤生物治疗,E-mail:yangming745@163.com。
通讯作者:张惠中(1961-),教授,主任医师,博士生导师,研究方向:肿瘤分子诊断及治疗靶点应用研究,E-mail:huizz328@163.com。
更新日期/Last Update: 2016-10-30