[1]李腾达,龙曙萍,徐贵霞,等.CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及相关细胞因子的影响与临床意义[J].现代检验医学杂志,2016,31(05):38-41.[doi:10.3969/j.issn.1671-7414.2016.05.010]
　LI Teng-da,LONG Shu-ping,XU Gui-xia,et al.Affection of CD26/DPP4 on CD4+T Cells and Relative Cytokines in Patients with Crytococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2016,31(05):38-41.[doi:10.3969/j.issn.1671-7414.2016.05.010]
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CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及相关细胞因子的影响与临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
38-41
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Affection of CD26/DPP4 on CD4+T Cells and Relative Cytokines in Patients with Crytococcal Meningitis and Its Clinical Significance
文章编号:
1671-7414(2016)05-038-04
作者:
李腾达1; 龙曙萍1; 徐贵霞1; 刘 云1; 张薇薇1; 钱 琤2; 黄元兰3; 秦 琴1; 陈孙孝4; 邓安梅1
1.第二军医大学长海医院,上海 200433;
2.解放军100医院,江苏苏州 215007;
3.解放军455医院,上海 200052;
4.第二军医大学长征医院,上海 200003
Author(s):
LI Teng-da1; LONG Shu-ping1; XU Gui-xia1; LIUYun1; ZHANG Wei-wei1; QIAN Cheng2;  HUANG Yuan-lan3; QIN Qin1; CHEN Sun-xiao4; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China;
2.No.100 Hospital of PLA,Jiangsu Suzhou 215007,China;
3.No.455 Hospital of PLA,Shanghai 200052,China;
4.Changzheng Hospital Affiliated to Second M
关键词:
隐球菌脑炎;  表面抗原分化簇26/二肽酶4;  白介素-17;  白介素-4;  干扰素-γ
分类号:
R519.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.05.010
文献标志码:
A
摘要:
目的 分析CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及其细胞因子的影响与临床意义。方法 收集2011年8月～2015年12月在上海长海医院和长征医院确诊的36例隐球菌脑膜炎患者及36例同期健康者外周血,以密度梯度离心法分离外周血单个核细胞(PBMC),流式细胞术分选CD26+CD4+T,CD26-CD4+T细胞群,反转录酶-聚合酶链锁反应(RT-PCR)检测细胞因子表达水平,以Pearson系数表示DPP4活性,CD26+CD4+T(%)与APACHE II评分,IL-17,TNF-α,IL-4,IFN-γ的相关性。结果 实验组与对照组CD26+CD4+T(%)为13.35±3.83 vs 8.39±2.14,t=6.78,P<0.000 1; DPP4活性为50.89±17.21 mU/ml vs 73.83±20.24 mU/ml,t=5.18,P<0.000 1,差异具有统计学意义。实验组CD26+CD4+T(%)与APACHE II评分,IL-17,TNF-α呈正相关(r=0.431,0.564,0.688,P=0.003 8,0.001,0.004 6); DPP4活性与APACHE II评分,IL-17,TNF-α,IFN-γ呈负相关(r=-0.544,-0.489,-0.678,-0.734; P均<0.001)。结论 CD26/DPP4可能通过调节Th细胞亚群参与隐球菌脑膜炎致病过程,是潜在疾病治疗靶点和预测指标。
Abstract:
Objective To analyze the affection and clinicalsignificance of CD26/DPP4 on CD4+T cells and its cytokines in patients with Cry tococcal Meningitis.Methods Peripheral blood was collected from 36 patients diagnosed with Crytococcal Meningitis in ChanghaiHospital and Changzheng Hospital,Shanghai from August,2011 to December,2015,meanwhile 36 health controls' was also acquired.Peripheral blood mononuclearcell(PBMC)was separated by density gradient centrifugation,CD26+CD4+T and CD26-CD4+T cell groups were classified by Flow Cytometry,the expression level ofcytokines was tested by reverse transcriptase-polymerase chain reaction(RT-PCR).The correlation between DPP4 activity,CD26+CD4+T(%)and APACHE II score,IL-17,TNF-α,IL-4,IFN-γ was measured by Pearson coefficient.Results CD26+CD4+T(%)between experimental and control groups was 13.35±3.83 vs 8.39±2.14(t=6.78,P<0.000 1).DPP4 activity was 50.89±17.21 mU/ml vs 73.83±20.24 mU/ml(t=5.18,P<0.000 1),with statistically significant differences.In experimental groups,CD26+CD4+T(%)was positively related with APACHE II score,IL-17,TNF-α(r=0.431,0.564,0.688,P=0.003 8,0.001,0.004 6).DPP4 activity was negatively interrelatedwith APACHE II score,IL-17,TNF-α,IFN-γ(r=-0.544,-0.489,-0.678,-0.734; P<0.001).Conclusion CD26/DPP4 may be involved in the pathogenesis of Crytococcal Meningitis through regulation of Th subgroups,and it was the potential therapeutic target and the predicted marker ofthe disease.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科技项目(BWS14J023,12MA056,15ZD009,15XD007),美捷登基金(MJR20150019)。
作者简介:李腾达(1990-),女,在读硕士生,主要从事感染免疫研究,E-mail:
更新日期/Last Update: 2016-10-30