[1]周晓冬,刘会利,赵汉东,等.原发性胆汁性胆管炎患者血清自身抗体及生化指标的特征分析[J].现代检验医学杂志,2016,31(06):32-36.[doi:10.3969/j.issn.1671-7414.2016.06.009]
　ZHOU Xiao-dong,LIU Hui-li,ZHAO Han-dong,et al.Characteristic Analysis of Sera of Autoantibodies and Biochemical Indexes of Patients with Primary Biliary Cholangitis[J].Journal of Modern Laboratory Medicine,2016,31(06):32-36.[doi:10.3969/j.issn.1671-7414.2016.06.009]
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原发性胆汁性胆管炎患者血清自身抗体及生化指标的特征分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
32-36
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Characteristic Analysis of Sera of Autoantibodies and Biochemical Indexes of Patients with Primary Biliary Cholangitis
作者:
周晓冬; 刘会利; 赵汉东; 刘红莉
西安市第八医院检验科,西安 710061
Author(s):
ZHOU Xiao-dong; LIU Hui-li; ZHAO Han-dong; LIU Hong-li
Department of Clinical Laboratory,the Eighth Hospital of Xi'an,Xi'an 710061,China
关键词:
原发性胆汁性胆管炎;  病毒性肝病;  自身抗体;  抗线粒体抗体
分类号:
R575.7; R446.11
DOI:
10.3969/j.issn.1671-7414.2016.06.009
文献标志码:
A
摘要:
目的 探讨原发性胆汁性胆管炎(PBC)患者血清自身抗体及生化指标的特征及临床意义。方法 采用间接免疫荧光法检测64例PBC,81例病毒性肝病,50例健康体检者三组血清中的抗核抗体(ANA)、抗线粒体抗体(AMA); 采用免疫印迹法检测三组的自身免疫肝病抗体(AMA-M2,M2-3E,抗Sp100抗体,抗PML抗体,抗gp210抗体,抗LKM-1抗体,抗LC-1抗体,抗SLA/LP 抗体,Ro52); 采用全自动生化分析仪检测其生化指标。结果 PBC组ANA和AMA阳性率明显高于病毒性肝病组,差异有统计学意义(χ2 =56.07,54.72,均P<0.01); PBC组自身免疫肝病抗体(AMA-M2,M2-3E,抗Sp100抗体,抗gp210抗体,Ro52)阳性率与病毒性肝病组比较,差异有统计学意义(χ2=9.06～98.70,均P<0.01); PBC组生化指标丙氨酸氨基转移酶(ALT)与病毒性肝病组比较,差异无统计学意义(F=6.069,P>0.05),其余总胆红素(TBIL)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和谷氨酰转肽酶(GGT)、总胆固醇(TC)与病毒性肝病组及健康对照组比较差异均有统计学意义(F=6.069～35.56,均P<0.05); AMA-M2阳性和阴性的PBC患者之间的生化指标比较差异无统计学意义(F=0.425～2.775,P>0.05); 抗gp210抗体阳性患者的TC高于阴性患者,差异有统计学意义(F=4.521,P=0.044)。结论 自身抗体ANA,AMA,AMA-M2,M2-3E,抗Sp100抗体,抗gp210抗体有助于PBC的早期诊断,结合生化指标则有利于PBC和病毒性肝病的鉴别诊断和疗效观察。此外,抗gp210抗体阳性与PBC患者的TC有一定的相关性。
Abstract:
Objective To investigate the primary biliary cholangitis(PBC)characteristics of patients with serum autoantibodies and biochemical indexes and clinical significance.Methods With indirect immunofluorescence method,the serum antinuclear antibody(ANA)and anti mitochondrialantibody(AMA)in 64 cases of PBC,81 cases of virusof liver disease and 50 cases of healthy persons were measured.Used immunoblot assay to detect autoimmune liver disease antibody(AMA-M2, M2-3E and Sp100 antibody and anti PML antibody and anti-gp210 antibody and anti LKM-1 antibody,antibody LC-1,anti SLA/LP antibody,Ro52 of threegroups,and used automatic biochemical analyzer to detect the biochemical indicators.Results The positive rate of ANA and AMA in PBC group was significantly higher than that of viral liver disease group,the difference was statistically significant(χ2=56.07,54.72,P<0.01).PBC group of autoimmune liver diseases(AMA-M2,M2-3E,anti Sp100 antibody,anti gp210 antibody,Ro52)compared with the positive rate of viral liver disease group,was statistically significant the difference(χ2=9.06～98.70,P<0.01).Group PBC biochemical indexes of alanine aminotransferase(ALT)compared with viral hepatitis group,thedifference was not statistically significant(F=6.069,P>0.05),the totalbilirubin(TBIL)and aspartate aminotransferase(AST),alkaline phosphatase(ALP)and glutamyl transpeptidase(GGT),total cholesterol(TC)and the differencebetween viral hepatitis and healthy control group were statistically significant(F=6.069～35.56,P<0.05); between AMA-M2 positive and negative patientswith PBC students,there was no statistical significance(F=0.425～2.775,P>0.05).The difference was statistically significant(F=4.521,P=0.044)in TC positive patients with anti gp210 antibody.Conclusion The autoantibodies included ANA,AMA,AMA-M2,M2-3E,Sp100,gp210 antibody are helpful for the early diagnosis of PBC,and differentiate the diagnosis and treatment between PBC and viral hepatitis.Gp210 antibody positive is correlated with TC in PBC patients.

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备注/Memo

备注/Memo:
作者简介:周晓冬(1976-),女,本科,主管检验技师,主要从事自身免疫性肝病和临床微生物学检验工作,E-mail: wrsd001@sina.com。 通讯作者:刘红莉,女,博士,主任技师,硕士研究生导师,E-mail:liuhonglili@sina.com。
更新日期/Last Update: 2016-12-20