[1]韩泽平,谢杏仪,何金花,等.马钱子碱对人慢性粒细胞白血病KCL-22细胞的增殖及凋亡作用[J].现代检验医学杂志,2016,31(06):66-69，73.[doi:10.3969/j.issn.1671-7414.2016.06.018]
　HAN Ze-ping,XIE Xing-yi,HE Jin-hua,et al.Effects of Brucine on Proliferation and Apoptosis of Chronic Myelogenous Leukemia KCL-22 Cell Line[J].Journal of Modern Laboratory Medicine,2016,31(06):66-69，73.[doi:10.3969/j.issn.1671-7414.2016.06.018]
点击复制

马钱子碱对人慢性粒细胞白血病KCL-22细胞的增殖及凋亡作用()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
66-69，73
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Effects of Brucine on Proliferation and Apoptosis of Chronic Myelogenous Leukemia KCL-22 Cell Line
作者:
韩泽平; 谢杏仪; 何金花; 黎毓光; 吕钰冰; 周嘉彬
广州市番禺区中心医院检验科,广州 511400
Author(s):
HAN Ze-ping; XIE Xing-yi; HE Jin-hua; LI Yu-guang; LV Yu-bing; ZHOU Jia-bin
Department of Clinical Laboratory, Panyu Center Hospital in Guangzhou City,Guangzhou 511400,China
关键词:
马钱子碱:慢性粒细胞白血病:KCL-22细胞:增殖抑制:凋亡
分类号:
R557.3; R392.12
DOI:
10.3969/j.issn.1671-7414.2016.06.018
文献标志码:
A
摘要:
目的 探索马钱子碱对人慢性粒细胞白血病KCL-22细胞的增殖抑制及诱导凋亡作用,为白血病的治疗提供新的方向。方法 体外培养白血病KCL-22细胞株,采用MTS法检测不同浓度马钱子碱对KCL-22细胞增殖的影响; 通过FITC-Annexin V/PI双染法检测不同浓度马钱子碱处理后细胞的凋亡作用; 运用Western blotting法检测凋亡相关蛋白的表达水平变化。结果 马钱子碱可明显抑制KCL-22细胞增殖,且具有浓度和时间依赖性。马钱子碱可诱导KCL-22细胞发生凋亡,并以早期凋亡为主,在50～400 μg/ml范围内呈剂量效应关系。经马钱子碱处理后,Bcl-2蛋白表达降低、Bax和Cyt-C蛋白表达增高,Caspase-9,Caspase-3均被切割活化。结论 马钱子碱可显著抑制慢性白血病KCL-22细胞增殖,并可能通过调控Bax/Bcl-2平衡、释放Cyt-C及激活Caspase-3,9依赖的内源性线粒体途径诱导细胞凋亡。
Abstract:
Objective To study the inhibition and apoptosisof brucine on chronic myelogenous leukemia KCL-22 cells,and then provide a new direction for the treatment of CML.Methods Different concentrations of brucine was added into the KCL-22 cell line in vitro.The inhibition effect of brucine on growth of KCL-22 cells was measured by MTS method.FITC-Annexin-V/PI double labeling method was used to assay the apoptosis rateof KCL-22 after different concentration of brucine treatment.The expression ofapoptosis-related protein was assayed with Western blotting.Results Brucine displayed significant anti-proliferative effect on KCL-22 cells in a dose-and time-dependent manner.Brucine could significantly induced KCL-22 cell apoptosis in the concentration range from 50 μg/ml to 400 μg/ml,mainly for early apoptosis.Western blotting showed that the expression ofBcl-2 was reduced,the expression of Bax and Cyt-C were increased,and the Caspase-9, Caspase-3 were activated.Conclusion Brucinecould inhibits the proliferation of KCL-22 cells,and induce apoptosis possiblythough endogenous mitochondrial pathway by regulating the Bax/Bcl-2 balance,releasing Cyt-C and activating Caspase-9 and Caspase-3.

参考文献/References:

[1] 唐 敏,伍冠一,朱 婵,等.马钱子碱镇痛研究进展[J].中草药,2014,45(12):1791-1795. Tang M,Wu GY,Zhu C,et al.Research progress on analgesic properties of brucine[J].Chinese Traditional and Herbal Drugs,2014,45(12):1791-1795.
[2] 杨福伟,王 辰,王光明,等.Brucine抗肿瘤的分子水平研究进展[J].中国老年学杂志,2014,34(17):5022-5024. Yang FW,Wang C,Wang GM,et al.Research progre-ss on anti-tumor molecular level of Brucine[J].Chinese Journal of Gerontology,2014,34(17):5022-5024.
[3] Shu G,Mi X,Cai J,et al.Brucine,an alkaloid from seeds of Strychnos nux-vomica Linn,represses hepatocellular carcinoma cell migration and metastasis:the role of hypoxia inducible factor 1 pathway[J].Toxicol Lett,2013,222(2):91-101.
[4] Li P,Zhang M,Ma WJ,et al.Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer[J].Chin J Integr Med,2012,18(8):605-609.
[5] Qiu JX,He YQ,Wang Y,et al.Plumbagin induces the apoptosis of human tongue carcinoma cells through the mitochondria-mediated pathway[J].Med Sci Monit Basic Res,2013(19):228-236.
[6] Thompson PA,Kantarjian HM,Cortes JE.Diagnosis and treatment of chronic myeloid leukemia in 2015[J].Mayo Clin Proc,2015,90(10):1440-1454.
[7] 辛 菲,魏 武,纪爱芳,等.马钱子碱诱导人单核细胞白血病THP-1细胞凋亡及作用机制[J].中国实验血液学杂志,2014,22(3):681-686. Xin F,Wei W,Ji AF,et al.Inducing-apoptosis effect of brucine onhuman monocytic leukemia cell line THP-1 and its mechanism[J].Journal of Experimental Hematology,2014,22(3):681-686.
[8] 李仙仙,魏 武,纪爱芳,等.马钱子碱对白血病细胞株HL-60的作用研究[J].白血病·淋巴瘤,2013,22(10):593-596. Li XX,Wei W,Ji AF,et al.Effects of brucine on chronic myeloid leukemia cell line HL-60[J].Journal of Leukemia & Lymphoma,2013,22(10):593-596.
[9] 王海丽,魏 武,纪爱芳,等.马钱子碱对人白血病细胞株K562细胞的诱导凋亡作用[J].中国实验血液学杂志,2011,19(3):630-633. Wang HL,We W,Ji AF,et al.Apoptosis-inducing effects of brucine on human chronic myeloid leukemia cell line K562[J].Journal of Experimental Hematology,2011,19(3):630-633.
[10] Ola M S,Nawaz M,Ahsan H.Role of Bcl-2 family proteins and caspases in the regulation of apoptosis[J].Mol Cell Biochem,2011,351(1/2):41-58.
[11] Ashkenazi A.Targeting the extrinsic apoptotic pathway in cancer:lessons learned and future directions[J].J Clin Invest,2015,125(2):487-489.
[12] Goldar S,Khaniani MS,Derakhshan SM,et al.Molecular mechanisms of apoptosis and roles in cancer development and treatment[J].Asian Pac J Cancer Prev,2015,16(6):2129-2144.

备注/Memo

备注/Memo:
基金项目:广东省建设中医药强省项目(20141230); 广州市番禺区科技和信息化局项目(2014-Z03-65)。 作者简介:韩泽平(1987-),男,本科,检验师,主要从事肿瘤学、生物信息学、分子生物学等研究工作,E-mail:hanzeping1987@aliyun.com。 通讯作者:谢杏仪,E-mail:huangwenxuanok@163.com。
更新日期/Last Update: 2016-12-20