[1]杨晓红,徐庆雷,马小波,等.老年哮喘患者支气管肺泡灌洗液中粒细胞巨噬细胞集落刺激因子水平变化及意义[J].现代检验医学杂志,2016,31(06):70-73.[doi:10.3969/j.issn.1671-7414.2016.06.019]
　YANG Xiao-hong,XU Qing-lei,MA Xiao-bo,et al.Level of Granulocyte Macrophage Colony Stimulating Factor in Bronchoalveolar Lavage Fluid from Elderly Patients with Asthma Bronchial and Their Significance[J].Journal of Modern Laboratory Medicine,2016,31(06):70-73.[doi:10.3969/j.issn.1671-7414.2016.06.019]
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老年哮喘患者支气管肺泡灌洗液中粒细胞巨噬细胞集落刺激因子水平变化及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
70-73
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Level of Granulocyte Macrophage Colony Stimulating Factor in Bronchoalveolar Lavage Fluid from Elderly Patients with Asthma Bronchial and Their Significance
作者:
杨晓红1; 徐庆雷1; 马小波1; 张巍1; 刘玲玲1; 王加平2
1.沭阳县人民医院检验科,江苏沭阳 223600; 2.东海县人民医院检验科,江苏东海 222300
Author(s):
YANG Xiao-hong1; XU Qing-lei1; MA Xiao-bo1; ZHANG Wei1; LIU Ling-ling1; WANG Jia-ping1
1.Department of Clinical Laboratory,People's Hospital of Shuyang, Jiangsu Shuyang 223600,China; 2.Department of Clinical Laboratory, People's Hospital of Donghai,Jiangsu Donghai 222300,China
关键词:
粒细胞巨噬细胞集落刺激因子;  中性粒细胞;  髓过氧化物酶;  支气管肺泡灌洗液;  哮喘
分类号:
R562.25; R392.12
DOI:
10.3969/j.issn.1671-7414.2016.06.019
文献标志码:
A
摘要:
目的 探讨老年哮喘患者支气管肺泡灌洗液(BALF)中粒细胞巨噬细胞集落刺激因子(GM-CSF)水平变化及其临床意义。方法 通过支气管镜肺泡灌洗,收集2015年1月～12月确诊的39例老年哮喘患者急性发作期和缓解期以及35例对照者的BALF标本。显微镜下计数BALF中中性粒细胞(NEU)占白细胞百分比,采用酶联免疫吸附试验检测BALF中GM-CSF和髓过氧化物酶(MPO)水平。统计学处理采用LSD-t检验和Pearson相关分析。结果 老年哮喘患者BALF中GM-CSF水平急性发作期(184.6±35.0 ng/L)显著高于缓解期(132.9±31.4 ng/L)及对照组(125.0±26.1 ng/L ),差异有统计学意义(t=6.778,8.115,P均<0.01); MPO水平急性发作期(43.9±11.7 ng/L)显著高于缓解期(24.5±9.9 ng/L)及对照组(21.8±8.5 ng/L ),差异有统计学意义(t=7.804,9.080,P均<0.01); NEU百分比急性发作期(42.1%±9.6%)显著高于缓解期(31.6%±8.2%)及对照组(28.8%±7.5%),差异有统计学意义(t=5.127,6.497,P均<0.01)。老年哮喘患者缓解期BALF中GM-CSF和MPO水平及NEU百分比与对照组比较,差异均无统计学意义(t=1.153～1.840,P均>0.05)。老年哮喘患者急性发作期BALF中GM-CSF和NEU百分比及MPO水平均呈正相关(r=0.554,0.725,P均<0.01),NEU百分比与MPO呈正相关(r=0.569,P<0.01)。结论 老年哮喘患者急性期BALF中GM-CSF水平升高,与疾病的发生发展关系密切,可作为病情的有效监测指标。
Abstract:
Objective To elucidate clinical significanceof the varied level of granulocyte macrophage colony stimulating factor(GM-CSF)in bronchoalveolar lavage fluid(BALF)from elderly patients with asthma bronchial.Methods From January to December 2015,39 asthma bronchial patients and 35 controls were enrolled into the current study.BALF wereobtained through the bronchoscope alveolar lavage.Cells were counted under themicroscope.GM-CSF and myeloperoxidase(MPO)were detected by ELISAs.LSD-t test and Pearson correlation were used for statistical analysis.Results There was significant difference in GM-CSF levels of asthmabronchial patients in attack stage(184.6±35.0 ng/L),remission stage(132.9±31.4 ng/L)and control groups(125.0±26.1 ng/L).Through pairwise comparison,the GM-CSF levels in asthma bronchial patients in attack stage were significantly higher than that in remission stage(t=6.778,P<0.01)and controlgroups(t=8.115,P<0.01).The levels of MPO in the 3 groups were 43.9±11.7 ng/L,24.5±9.9 ng/L,21.8±8.5 ng/L,respectively.Comparison to remission and normal control groups,the MPO levels were markedly higher in attack stage(t=7.804,9.080,both P<0.01).The percentages of neutrophils were42.1%±9.6%,31.6%±8.2% and 28.8%±7.5% respectively in the 3 groups,respectively.The percentages of neutrophils in attack stage were markedly higherthan that in remission stage(t=5.127,P<0.01)as well as normal control groups(t=6.497,P<0.01).The levels of GM-CSF and MPO and the percentages of neutrophils were no significant differences between asthma bronchial patients in remission stage and in control groups(t=1.153～1.840,all P>0.05).Furthermore,the result of this study showed a positively correlated between the GM-CSF and the percentages of neutrophils(r=0.554,P<0.01)as well as the MPO(r= 0.725,P<0.01)and a positive correlation between thepercentages of neutrophils and the MPO(r= 0.569,P<0.01)in elderly patients with asthma bronchial in attack stage.Conclusion These results indicated that GM-CSF was higher in asthma bronchial patients in attack stage,and suggesting that GM-CSF might be involved in the pathological process of asthma.GM-CSF may be used as an effective index to monitor the condition of elderly patients with asthma.

参考文献/References:

[1] Nakagome K,Matsushita S,Nagata M.Neutrophilic inflammation in severe asthma[J].Int Arch Allergy Immunol,2012,158(Suppl 1):96-102.
[2] Bruijnzeel Piet LB,Uddin M,Koenderman L.Targeting neutrophilic inflammation in severe neutrophilic asthma:can we target the disease-relevant neutrophil phenotype?[J].J Leukoc Biol,2015,98(4):549-556.
[3] Manni ML,Robinson KR,Alcorn JF.A tale of two cytokines:IL-17 and IL-22 in asthma and infection[J].Expert Rev Respir Med,2014,8(1):25-42.
[4] R(¨overo)sler B,Herold S.Lung epithelial GM-CSF impro-ves host defense function and epithelial repair in influenza virus pneumonia-a new therapeutic strategy?[J].Molecular and Cellular Pediatrics,2016,3(1):29.
[5] Khajah M,Millen B,Cara DC,et al.Granulocyte-macrophage colony-stimulating factor(GM-CSF):a chemoattractive agent for murine leukocytes in vivo[J].J Leukoc Biol,2011,89(6):945-953.
[6] Davies MJ.Myeloperoxidase-derived oxidation:mechanisms of biological damage and its prevention[J].J Clin Biochem Nutr,2011,48(1):8-19.
[7] 中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南(支气管哮喘的定义、诊断、治疗和管理方案)[J].中华结核和呼吸杂志,2008,31(3):177-185. Asthma Committee of Chinese Society of Respirology.Guidelines for the prevention and treatment of bronchial asthma(the definition,diagnosis,treatment and management of bronchial asthma)[J].Chin J Tuberc Respir Dis,2008,31(3):177-185.
[8] Bateman ED,Hurd SS,Barnes PJ,et al.Global strategy for asthma management and prevention:GINA executive summary[J].Eur Respir J,2008,31(1):143-178.
[9] Sylvain-Prévost S,Ear T,Simard FA,et al.Activation of TAK1 by chemotactic and growth factors,and its impact on human neutrophil signaling and functional responses[J].J Immunol,2015,195(11):5393-5403.
[10] Zahran N,Sayed A,William I,et al.Neutrophil apoptosis:impact of granulocyte macrophage colony stimulating factor on cell survival and viability in chronic kidney disease and hemodialysis patients[J].Arch Med Sci,2013,9(6):984-989.
[11] Holt PG.Antigen presentation in the lung[J].Am J Respr Crit Care Med,2000,162(4):151-156.
[12] AccianiI TH,Suzuki T,Trapnell BC,et al.Epidermal growth factor receptor signalling regulates granulocyte-macrophage colony-stimulating factor production by airway epithelial cells and established allergic airway disease[J].Clin Exp Allergy,2016,46(2):317-328.
[13] Lee HS,Park DE,Song WJ,et al.Effect of 1.8-Cineole in dermatophagoides pteronyssinus-stimulated bronchial epithelial cells and mouse model of asthma[J].Biol Pharm Bull,2016,39(6):946-952.
[14] 刘 禹,李志伟,杨 旭,等.哮喘气道中性粒细胞性炎症表型的临床意义[J].中华肺部疾病杂志(电子版),2015,8(2):170-173. Liu Y,Li ZW,Yang X,et al.Clinical significance of neutrophilic airwayinflammatory phenotype in patients with asthma[J].Chin J Lung Dis(ElectronicEdition),2015,8(2):170-173.
[15] Selsted ME,Miller CW,Novotny MJ,et al.Molecular analysis of myeloperoxidase deficiency shows heterogeneous patterns of the complete deficiency statemanifested at the genomic,mRNA and protein levels[J].Blood,1993,82(4):1317-1322.
[16] 喻红波,刘 阳,罗俊卿,等.髓过氧化物酶在非ST段抬高急性冠状动脉综合征诊断和预后中的临床应用[J].现代检验医学杂志,2015,30(3):77-79. Yu HB,Liu Y,Luo JQ,et al.Clinical application of myeloperoxidase in diagnosis and prognosis of non-ST elevation acute cornary syndromes[J].Journal of Modern Laboratory Medicine,2015,30(3):77-79.
[17] Salonen I,Huttunen K,Hirvonen MR,et al.Serum myeloperoxidase is independent of the risk factors of atherosclerosis[J].Coron Artery Dis,2012,23(4):251-258.
[18] 袁晓梅,明 晓,王 静.氨溴索对哮喘大鼠炎性因子的影响及其作用机制[J].中国医院药学杂志,2012,32(11):837-840. Yuan XM,Ming X,Wang J.Effect of ambroxol on airway inflammation and its mechanisms of actions in asthmatic rats[J].Chinese Journal of Hosptial Pharmacy,2012,32(11):837-840.

备注/Memo

备注/Memo:
作者简介:杨晓红(1969-),女,医学学士,副主任技师,专业:临床医学检验,E-mail:992898662@qq.com。 通讯作者:王加平,女,副主任技师,研究方向:临床免疫学检验及临床相关疾病研究,E-mail:jiapingwang8080@126.com。
更新日期/Last Update: 2016-12-20