[1]周秋媛,王红莉,李虹,等.NRP-1和NRP-2在结直肠癌组织中的表达水平及临床意义[J].现代检验医学杂志,2016,31(06):85-87，91.[doi:10.3969/j.issn.1671-7414.2016.06.024]
　ZHOU Qiu-yuan,WANG Hong-li,LI Hong,et al.Serum NRP-1 and NRP-2 Expression in Colorectal Cancer Patients and Its Relationship with Clinical Pathology[J].Journal of Modern Laboratory Medicine,2016,31(06):85-87，91.[doi:10.3969/j.issn.1671-7414.2016.06.024]
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NRP-1和NRP-2在结直肠癌组织中的表达水平及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
85-87，91
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Serum NRP-1 and NRP-2 Expression in Colorectal Cancer Patients and Its Relationship with Clinical Pathology
作者:
周秋媛; 王红莉; 李虹; 李伟; 徐艳华
恩施州中心医院病理科,湖北恩施 445000
Author(s):
ZHOU Qiu-yuan; WANG Hong-li; LI Hong; LI wei; XU Yan-hua
Department of Pathology, the Central Hospital of Enshi Autonomous Prefecture,Hubei Enshi 445000,China
关键词:
结直肠癌;  神经纤毛蛋白-1;  神经纤毛蛋白-2;  临床病理;  表达水平
分类号:
R735.3; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.06.024
文献标志码:
A
摘要:
目的 探讨神经纤毛蛋白1(neuropilin-1,NRP-1)和NRP-2在结直肠癌组织中的表达水平及临床意义。方法 选取2014年12月～2015年9月期间湖北省恩施州中心医院65例结直肠癌患者和65例肠息肉患者作为研究对象,选择于同期健康检查的65例健康女性作为正常对照组。采用实时荧光定量技术(RT-PCR)检测入组对象血清NRP-1和NRP-2水平。结果 经RT-PCR检测,结直肠癌组NRP-1水平(36.51±4.62 μg/ml)高于肠息肉组(8.19±4.08 μg/ml)和健康对照组(7.92±4.05 μg/ml),差异有统计学意义(F=50.32,P=0.000),结直肠癌组NRP-2 mRNA水平(24.59±3.26 μg/ml)高于肠息肉组(5.47±1.11 μg/ml)和健康对照组(5.19±1.05 μg/ml),差异有统计学意义(F=49.36,P=0.000)。肠息肉组和健康对照组NRP-1和NRP-2 mRNA水平比较,差异无统计学意义(t=1.15,1.07,P=0.762,0.789)。经RT-PCR检测,随着患者病理分期的增加、分化程度的降低,结直肠癌组血清NRP-1和NRP-2 mRNA水平呈升高趋势,伴肝转移和淋巴结转移的结直肠癌患者,血清NRP-1和NRP-2 mRNA水平高于未出现肝转移和淋巴结转移的结直肠癌患者(P<0.05)。结直肠癌患者血清NRP-1和NRP-2 mRNA水平呈正相关(r=0.415,P=0.015)。结论 结直肠癌血清NRP-1,NRP-2水平高于肠息肉及健康人群。血清NRP-1和NRP-2水平与结直肠癌的临床病理及预后密切相关,NRP-1,NRP-2可能共同起作用。
Abstract:
Objective To study serum NRP-1 and NRP-2 expression in colorectal cancer patients and its relationship with clinical pathology.Methods Between December 2014 and September 2015,in the Central Hospital of Enshi Autonomous Prefecture 65 cases of colorectal cancerpatients and 65 patients with Intestinal polyps were selected as the research object,and between December 2014 and September 2015 60 cases of healthy womenfor health examination in the Central Hospital of Enshi Autonomous Prefecture were selected as normal control group.The real-time fluorescence quantitative technology(RT-PCR)was used to test serum NRP-1 and NRP-2 level.Results By RT-PCR detection,NRP-1 level of colorectal cancer group(36.51±4.62 μg/ml)was higher than that of colon polyps group(8.19±4.08 μg/ml)and thehealthy controls(7.92±4.05 μg/ml),and the difference was statistically significant(F= 50.32,P=0.000).NRP-2 mRNA level of colorectal cancer group(24.59±3.26 μg/ml)was higher than that of colon polyps group(5.47±1.11 μg/ml)and the healthy controls(5.19±1.05 μg/ml),and the difference was statistically significant(F=49.36,P=0.000).And between NRP-1 and NRP-2 mRNA comparison of intestinal polyp group and healthy controls,there was no statistically significant difference(t=1.15,1.07,P=0.762,0.789).By RT-PCR detection,with theincrease of patients with pathological staging and differentiation degree reducing,serum NRP-1 and NRP-2 mRNA of colorectal cancer group had the rise trend.NRP-1 and NRP-2 mRNA of colorectal cancer patients with liver metastasis andlymph node metastasis were higher than colorectal cancer patients without liver metastasis and lymph node metastasis(P<0.05).Serum NRP-1 and NRP-2 mRNA levels of colorectal cancer patients were positively correlated(r=0.415,P=0.415).Conclusion Serum NRP-1 and NRP-2 levels ofbreast cancer were higher than that of benign breast lesions and healthy people.Serum NRP-1 and NRP-2 levels were closely related to the clinical pathologyand prognosis of breast cancer.Breast cancer radical operation could inhibit the expression of miR-574-5p and improve the patient's disease condition.SerumNRP-1 and NRP-2 levels of colorectal cancer were higher than intestinal polyps and healthy people.Serum NRP-1 and NRP-2 levels were closely associated with the clinical pathology and prognosis of colorectal cancer,and NRP-1 and NRP-2 could play the role together.

参考文献/References:

[1] 邹瞭南,吴畑华,刁德昌,等.三联疗法在结直肠癌合并梗阻治疗中的应用[J].中华胃肠外科杂志,2015,18(1):77-79. Zou LN,Wu TH,Diao DC,et al.Triple therapy in the application ofthe colorectal cancer complicated with obstruction[J].Chinese Journal of Gastrointestinal Surgery,2015,18(1):77-79.
[2] 张立达,白阳秋,杨玉秀.分泌性白细胞蛋白酶抑制剂在结肠癌中的作用[J].中华实验外科杂志,2015,32(7):1695-1697. Zhang LD,Bai YQ,Yang YX.Role of secretory leukocyte protease inhibitor expression in the tumorigenesis of colon cancer[J].Chinese Journal of Experimental Surgery,2015,32(7):1695-1697.
[3] Schmidt T,Strowitzki MJ,Reissfelder C,et al.Influence of age on resection of colorectal liver metastases[J].J Surg Oncol,2015,111(6):729-739.
[4] 陈晓露.白细胞介素4基因多态性与结直肠癌的易感性[J].现代检验医学杂志,2015,30(5):105-106,110. Chen XL.Interleukin-4 gene polymorphisms and colorectal cancer susceptibility[J].Journal of Modern Laboratory Medicine,2015,30(5):105-106,110.
[5] 董娟聪,张海芹,高 辉,等.神经纤毛蛋白1对非小细胞肺癌放射敏感性的影响[J].中华放射医学与防护杂志,2014,34(11):809-812. Dong JC,Zhang HQ,Gao H,et al.Effect of neuropilin-1 on radio-sensitivity of non-small cell lung carcinoma[J].Chinese Journal of Radiological Medicine and Protection,2014,34(11):809-812.
[6] 孙 瑾,王 亮,娄卫华,等.shRNA沉默NRP-1基因抑制鼻咽癌CNE-2Z细胞生长的体内外研究[J].中华耳鼻咽喉头颈外科杂志,2015,50(4):312-317. Sun J,Wang L,Lou WH,et al.NRP-1/shRNA silencing suppresses nasopharyngeal carcinoma cell growth in vitro and in vivo[J].Chinese Journal of Otorhinolaryngology Head and Neck Surgery,2015,50(4):312-317.
[7] 2014年NCCN结直肠癌临床指南更新要点[J].中国全科医学,2014,17(30):3539. Updating points of 2014 NCCN colorectal cancer clinical guidelines[J].Chinese General Practice,2014,17(30):3539.
[8] Liu Y,He G,Wang Y,et al.MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells[J].Toxicol Lett,2013,221(1):23-30.
[9] 王钦君,张红春,申娴娟,等.MicroRNAs作为结直肠癌潜在标记物的研究进展[J].现代检验医学杂志,2015,30(4):107-110,114. Wang QJ,Zhang HC,Shen XJ,et al.MicroRNAs as a potential marker in the progress of colorectal cancer[J].Journal of Modern Laboratory Medicine,2015,30(4):107-110,114.
[10] 安吉洋,宋锦宁,王军锋,等.大鼠弥漫性轴索损伤后突起坍塌蛋白Sema3A及其受体neuropilin-1的表达[J].西安交通大学学报(医学版),2014,35(6):753-759. An JY,Song JN,Wang JF,et al.Expressions of se-maphorin 3A and neuropilin-1 in the brain after diffuse axonal injury in rats[J].Journal of Xi'anJiaotong University(Medical Sciences),2014,35(6):753-759.
[11] McKenna CC,Munjaal RP,Lwigale PY.Distinct ro-les for neuropilin1 and neuropilin2 during mouse corneal innervation[J].PLoS One,2012,7(5):e37175.
[12] Maulik D,De A,Ragolia L,et al.Down-regulation of placental neuropilin-1 in fetal growth restriction[J].Am J Obstet Gynecol,2016,214(2):279.e1-9.
[13] Elahouel R,Blanc C,Carpentier G,et al.Pleiotrophin exerts its migration and invasion effect through the neuropilin-1 pathway[J].Neoplasia,2015,17(8):613-624.
[14] Pellet-Many C,Mehta V,Fields L,et al.Neuropilins 1 and 2 mediate neointimal hyperplasia and re-endothelialization following arterial injury[J].Cardiovasc Res,2015,108(2):288-298.
[15] Groppa E,Brkic S,Bovo E,et al.VEGF dose regulates vascular stabilization through Semaphorin3A and the Neuropilin-1+ monocyte/TGF-β1 paracrine axis[J].EMBO Mol Med,2015,7(10):1366-1384.

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备注/Memo

备注/Memo:
作者简介:周秋媛(1981-),女,硕士研究生,主治医师,主要擅长肿瘤分子病理学研究,Tel:13986060480,E-mail:394631987@qq.com。
更新日期/Last Update: 2016-12-20