[1]张华,詹颉,赵海,等.尿液HCMVDNA检测在儿童HCMV感染中的临床应用[J].现代检验医学杂志,2016,31(06):95-97.[doi:10.3969/j.issn.1671-7414.2016.06.027]
　ZHANG Hua,ZHAN Jie,ZHAO Hai,et al.Clinical Study on Detecting HCMV in Urine of Infants[J].Journal of Modern Laboratory Medicine,2016,31(06):95-97.[doi:10.3969/j.issn.1671-7414.2016.06.027]
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尿液HCMVDNA检测在儿童HCMV感染中的临床应用()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
95-97
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Clinical Study on Detecting HCMV in Urine of Infants
作者:
张华,詹颉,赵海,苏宝凤,苍金荣,宫艳艳,刘培培,杨瑞雪
陕西省人民医院检验科,西安 710068
Author(s):
ZHANG Hua,ZHAN Jie,ZHAO Hai,SU Bao-feng, CANG Jin-rong,GONG Yan-yan,LIU Pei-pei,YANG Rui-xue
Department of Clinical Laboratory,Shaanxi Provincial People'sHospital,Xi'an 710068,China
关键词:
HCMV;  DNA;  尿液
分类号:
R373.11; Q503
DOI:
10.3969/j.issn.1671-7414.2016.06.027
文献标志码:
A
摘要:
目的 对患儿尿液进行HCMV DNA荧光定量检测并对检测结果进行统计学分析,以了解HCMV在患儿各年龄段及各种疾病中的感染情况。方法 采用荧光定量PCR技术对2014年1月～2015年12月收集的1 492份尿液标本进行HCMV DNA荧光定量分析。结果 新生儿组、婴幼儿组、学龄前组和学龄期组之间的HCMV DNA检测结果比较差异有统计学意义(χ2=9.253～23.452,P均<0.01); 学龄期组与新生儿组比较差异有非常显著性统计学意义(χ2=23.452,P<0.001)。患儿常见几种疾病组中,肝功能异常组、支气管肺炎组和发热组之间差异无统计学意义(χ2=0.356～0.865,P均>0.05)。肝功能异常组、支气管肺炎组和发热组三组与早产儿组和高胆红素血症组比较差异有统计学意义(χ2=13.231,P均<0.01)。结论 新生儿和婴幼儿是人巨细胞病毒感染的高发人群,其中的早产儿和高胆红素血症患儿的HCMV DNA检出率较高应引起重视; 尿液HCMV DNA检测简便快速,标本留取方便,在婴幼儿CMV感染的诊断和治疗中具有重要意义。
Abstract:
Objective To study the infection state of HCMVin different age of children and co-infection with other diseases,detecting human cytomegalovirus(HCMV)in urine of 1 492 cases of children by fluorescence quantitative detection method.Then the detection results were retrospectively analyzed.Methods Collected 1 492 specimens of children'surine from January 2014 to December 2014.Detected HCMV by fluorescence quantitative method.Results For different ages with HCMV DNA detection results,there were significant statistical differences between school-age group,neonatal group,infant group and preschool group respectively(χ2=9.253～23.452,P<0.01).Especially there was very significant statisticaldifferences between neonatal group and school-age group(χ2=23.452,P<0.001).For different co-infection diseases with HCMV DNA detection results,there was not significant statistical difference between fever group and abnormal liver function group,bronchial pneumonia group respectively(χ2=0.356～0.865,P>0.05).Compared abnormal live function,bronchial pneumonia groupand fever three group with preterm group and high blood bilirubin group,the difference was statistically significant(χ2=13.231,P均<0.01).Conclusion The results showed that high infection rate of HCMV innewborn infants and infants.Should pay attention to high blood bilirubin and premature infant co-infection with HCMV.Urine specimen is rapid and convenientspecimen for detection HCMV of infants.It is great significance in diagnosis and treatment of infantile CMV infection.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:张 华(1966-),女,大专学历,主管检验技师,擅长临床化学、免疫等检验技术及相关研究。
更新日期/Last Update: 2016-12-20