[1]周龙荣,徐元宏.基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)分析在丝状真菌实验诊断中的应用[J].现代检验医学杂志,2017,32(01):5-8,105.[doi:10.3969/j.issn.1671-7414.2017.01.002]
　ZHOU Long-rong,XU Yuan-hong.Application of Matrix-assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS) in the Laboratory Diagnosis of Filamentous Fungi[J].Journal of Modern Laboratory Medicine,2017,32(01):5-8,105.[doi:10.3969/j.issn.1671-7414.2017.01.002]
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基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)分析在丝状真菌实验诊断中的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
5-8,105
栏目:
述评
出版日期:
2017-01-25

文章信息/Info

Title:
Application of Matrix-assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS) in the Laboratory Diagnosis of Filamentous Fungi
文章编号:
1671-7414(2017)01-005-05
作者:
周龙荣; 徐元宏
安徽医科大学第一附属医院检验科,合肥 230022
Author(s):
ZHOU Long-rong; XU Yuan-hong
Department ofClinical Laboratory, the First Affiliated Hospital of Anhui Medical University,Hefei 230022,China
关键词:
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS);  丝状真菌
分类号:
R379.2; Q503
DOI:
10.3969/j.issn.1671-7414.2017.01.002
文献标志码:
A
摘要:
基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF MS)是一种可以对多种微生物(包括细菌、酵母和丝状真菌)进行常规鉴定的强有力工具。具有快速、准确、高通量及成本低廉等特点,逐渐被用于丝状真菌感染的实验室诊断中。在丝状真菌的MALDI-TOF MS分析中,从样本制备到获得准确的鉴定结果,每个步骤都决定了医技人员能否及时为临床医生提供准确结果。现就待测样品的制备及MALDI-TOF MS技术对丝状真菌分析(包括鉴定、分型、抗真菌敏感性测试方面)的应用及前景作一系统综述,也将对其一些未来发展进行展望。
Abstract:
Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry(MALDI-TOF MS)is a powerful tool for routineidentification of a variety of microorganisms,including bacteria,yeast and filamentous fungi.Fast,accurate,high-throughput and low-cost characteristics,and gradually used for filamentous fungal infection in the laboratory diagnosis.In the MALDI-TOF MS analysis of filamentous fungi,from sample preparationto obtaining accurate identification results,each step determines whether the medical technician can provide accurate results for the clinician.This review will provide a systematic overview of the applications and prospects of filamentous fungal analysis(including identification,typing,and antifungal susceptibility testing)from preparation of the test sample to the MALDI-TOF MS technique,as well as a review of some of its future developments.

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备注/Memo

备注/Memo:
基金项目:国家基金委自然科学研究项目,项目编号:81171606。
作者简介:周龙荣(1991-),女,硕士,技师,主要从事微生物学研究,Tel:18655270851。
通讯作者:徐元宏,男,教授,主任技师,博士研究生导师,E-mail:xyhong1964@163.com。
更新日期/Last Update: 2017-01-20