[1]丁 海,高 硕,魏红霞,等.系统性红斑狼疮患者外周血SOCS-1基因甲基化的检测及意义[J].现代检验医学杂志,2017,32(01):34-37.[doi:10.3969/j.issn.1671-7414.2017.01.010]
　DING Hai,GAO Shuo,WEI Hong-xia,et al.Detection and the Significance of SOCS-1 Gene Methylation Status in Peripheral Blood of Systemic Lupus Erythematosus Patients[J].Journal of Modern Laboratory Medicine,2017,32(01):34-37.[doi:10.3969/j.issn.1671-7414.2017.01.010]
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系统性红斑狼疮患者外周血SOCS-1基因甲基化的检测及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
34-37
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Detection and the Significance of SOCS-1 Gene Methylation Status in Peripheral Blood of Systemic Lupus Erythematosus Patients
文章编号:
1671-7414(2017)01-034-04
作者:
丁 海; 高 硕; 魏红霞; 李 雷; 王庆飞
南京大学医学院附属鼓楼医院检验科,南京 210008
Author(s):
DING Hai; GAO Shuo; WEI Hong-xia; LI Lei; WANG Qing-fei
Department of Clinical Laboratory Medicine, Nanjing Drum Tower Hospital,School of Medicine,Nanjing University,Nanjing 210008,China
关键词:
系统性红斑狼疮;  细胞因子信号转导抑制蛋白-1;  基因甲基化
分类号:
R593.241; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.01.010
文献标志码:
A
摘要:
目的 建立外周血DNA中细胞因子信号转导抑制蛋白-1(SOCS-1)基因甲基化状态的检测方法,初步探讨其与系统性红斑狼疮的关系,为诊治系统性红斑狼疮提供依据。方法 收集在南京大学医学院附属鼓楼医院2015年1月～4月期间确诊的系统性红斑狼疮患者27例和健康体检者19例的全血标本,抽提外周血DNA,采用聚合酶链式反应结合琼脂糖凝胶电泳的方法分析全血DNA中SOCS-1基因启动子的甲基化状态。结果 在27例系统性红斑狼疮患者中,SOCS-1基因完全甲基化占44%(12/27),不完全甲基化占56%(15/27); 而健康体检者的SOCS-1基因完全甲基化占74%(14/19),不完全甲基化占26%(5/19)。与健康对照相比,SLE患者SOCS-1基因完全甲基化的比例明显较低,差异具有统计学意义(χ2=3.88,P=0.049)。结论 系统性红斑狼疮患者外周血DNA中可能存在SOCS-1基因低甲基化的状态,其与系统性红斑狼疮的发生、发展的关系及诊治中的意义值得进一步深入研究。
Abstract:
Objective To investigate the relationship between the systemic lupus erythematosus(SLE)and the SOCS-1 gene methylation status of the peripheral blood DNA,to provide the basis for diagnosis and treatmentof systemic lupus erythematosus.Methods Blood samples ofSLE patients(27 cases)and healthy group(19 cases)in January 2015 to Aprilwere collected and the DNA were extracted.Using polymerase chain reaction combining DNA agarose gel electrophoresis to detect the SOCS-1 gene methylationstatus.Results In patients with systemic lupus erythematosus SOCS-1 gene complete methylation accounted for 44%(12/27),incomplete methylation accounted for 56%(15/27).In healthy group SOCS-1 gene complete methylation accounted for 74%(14/19)and incomplete methylation accounted for 26%(5/19).The rate of complete methylation of SOCS-1 gene of SLE patients was lowerthan that of healthy group(χ2=3.88,P=0.049).ConclusionSLE patients may have lower SOCS-1 gene methylation status in the peripheral blood DNA,which is worth for further study.

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备注/Memo

备注/Memo:
基金项目:南京市医学科技发展专项基金(YKK13066)。
作者简介:丁 海(1991-),男,学士,技师,主要从事临床检验,Tel:15150538859,E-mail:312355934@qq.com。
通讯作者:王庆飞(1983-),男,博士,E-mail: wangqingfei1020@foxmail.com。
更新日期/Last Update: 2017-01-20