[1]柴春艳,王 甜,刘娅萍,等.WWOX蛋白及Bcl-2蛋白在支气管肺癌中的表达及临床意义[J].现代检验医学杂志,2017,32(01):61-65.[doi:10.3969/j.issn.1671-7414.2017.01.017]
　CHAI Chun-yan,WANG Tian,LIU Ya-ping,et al.Expression and Clinical Significance of WWOX Protein and BCL-2 Protein in Primary Lung Cancer[J].Journal of Modern Laboratory Medicine,2017,32(01):61-65.[doi:10.3969/j.issn.1671-7414.2017.01.017]
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WWOX蛋白及Bcl-2蛋白在支气管肺癌中的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
61-65
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Expression and Clinical Significance of WWOX Protein and BCL-2 Protein in Primary Lung Cancer
文章编号:
1671-7414(2017)01-061-05
作者:
柴春艳1; 王 甜1; 刘娅萍1; 李雅莉2; 徐邦强1; 赵 媛1
1.陕西省人民医院西院一病区, 西安 710028;
2.西安交通大学第二附属医院呼吸内科,西安 710004
Author(s):
CHAI Chun-yan1; WANG Tian1; LIU Ya-ping1; LI Ya-li2; XU Bang-qiang1; ZHAO Yuan1
1.Shaanxi Provincial People's Hospital,Xi'an 710068,China;
2.Department of Respiratory Medicine,the Second Affiliated Hospital of Medical School of Xi'an Jiaotong University,Xi'an 710004,China
关键词:
目的 探讨WWOX蛋白及Bcl-2蛋白在支气管肺癌(primary lung cancer)组织的表达及临床意义。方法 选取陕西省人民医院2010～2015年手术切除并经病理证实的原发性支气管肺癌标本76例; 其中肺鳞癌29例; 肺腺癌27例; 肺小细胞癌20例。正常对照组为7例癌旁正常肺组织和8例肺结核球。采用免疫组化SP(streptavidin-perosidase)法检测WWOX蛋白和Bcl-2蛋白在支气管肺癌组织和癌旁5 cm以上正常肺组织中的表达情况; 分析患者与正常对照组间水平差异及性别、年龄、肺癌
分类号:
R734.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.01.017
文献标志码:
A
摘要:
目的 探讨WWOX蛋白及Bcl-2蛋白在支气管肺癌(primary lung cancer)组织的表达及临床意义。方法 选取陕西省人民医院2010～2015年手术切除并经病理证实的原发性支气管肺癌标本76例,其中肺鳞癌29例,肺腺癌27例,肺小细胞癌20例。正常对照组为7例癌旁正常肺组织和8例肺结核球。采用免疫组化SP(streptavidin-perosidase)法检测WWOX蛋白和Bcl-2蛋白在支气管肺癌组织和癌旁5 cm以上正常肺组织中的表达情况,分析患者与正常对照组间水平差异及性别、年龄、肺癌组织病理类型、分化程度、临床分期、有无淋巴结转移、吸烟指数对WWOX蛋白和Bcl-2蛋白表达的影响。结果 ①肺癌组的WWOX蛋白阳性表达率(35.52%)明显低于癌旁正常肺组织组(73.33%,P＜0.05),肺癌组的Bcl-2蛋白阳性表达率(78.06%)明显高于癌旁正常对照组(23.75%,P＜0.05)。②男性患者中WWOX蛋白阳性表达率(21.43%)明显低于女性患者(52.94%),差异有统计学意义(χ2=8.146,P=0.04)。男性患者中Bcl-2蛋白阳性表达率(71.43%)明显高于女性患者(35.29%),差异有统计学意义(χ2=9.923,P=0.002)。③有淋巴结转移的肺癌组织中,WWOX蛋白阳性率(17.07%)明显低于无淋巴结转移组织(48.57%),差异有统计学意义(χ2=8.67,P=0.003)。有淋巴结转移的肺癌组织中,Bcl-2蛋白表达阳性率(68.29%)明显高于无淋巴结转移者(34.28%),差异有统计学意义(χ2=8.758,P=0.003)。④WWOX蛋白表达阳性率在吸烟指数≥400的患者中为15.63%,明显低于吸烟指数＜400的患者(47.73%),差异有统计学意义(χ2=8.48,P=0.003)。在吸烟指数≥400的患者中,Bcl-2蛋白表达阳性率为56.25%,明显高于吸烟指数＜400的患者(22.73%),差异有统计学意义(χ2=8.947,P=0.003)。⑤肺癌患者的年龄及肺癌组织病理类型、分化程度、临床分期对WWOX蛋白和Bcl-2蛋白的阳性表达率的影响差异均无统计学意义(P＞0.05)。⑥WWOX蛋白与Bcl-2蛋白的表达呈负相关。结论 在原发性支气管肺癌组织中WWOX蛋白低表达,Bcl-2蛋白高表达,两者表达呈负相关。
Abstract:
Objective Toexplore expression and clinical significance of WWOX protein and the Bcl-2 protein in the organization of bronchial lung cancer(primary lung cancer).Methods Chose 76 lung cancer patients with clear pathological diagnosis who were hospitalized in the Shaanxi Provincial People's Hospital from 2010 and 2015(including 29 cases of adenocarcinoma,27 cases of squamous cell carcinoma,and 20 cases of small cell carcinoma)and 7 cases of normal lung tissue,8 cases of lung tuberculosis.The expressions of WWOX protein,Bcl-2 protein and more that 5 cm normal lung tissue adjacent to carcinoma were measured by immunohistochemistry SP method.The expression difference between patients and normal control group and the influence of sex,age,pathological type,differentiation degree,clinical stage,lymph node metastasis,smoking index on the expression of WWOX protein and Bcl-2 protein in lung cancer were analyzed.Results ①The positive expression rate of WWOX protein in lung cancer group(35.52%)was significantly lower than that in normal lung tissue(73.33%,P＜0.05).The positive expression rate of Bcl-2 protein in lung cancer group(78.06%)was significantly higher than that in control group(23.75%,P＜0.05).②The positive expression rate of WWOX protein in male patients(21.43%)was significantly lower than that in female patients(52.94%),and the difference was statistically significant(χ2=8.146,P=0.04).The positive expression rate of Bcl-2 protein in male patients(71.43%)was significantly higher than that in female patients(35.29%),the difference was statistically significant(χ2=9.923,P=0.002).③In lung cancer with lymph node metastasis,the positive rate of WWOX protein(17.07%)was significantly lower than that in non-lymph node metastasis(48.57%),and the difference was statistically significant(χ2=8.67,P=0.003).In lung cancer with lymph node metastasis,the positive rate of Bcl-2 protein expression(68.29%)was significantly higher than that in non-lymph node metastasis(34.28%),and the difference was statistically significant(χ2=8.758,P=0.003).④The positive rate of expression of WWOX protein in patients whose smoking index≥400 and in patients that＜400 was 15.63% and 47.73%,respectively,the differences were significant(χ2=8.48,P=0.003).The positive rate of expression of Bcl-2 protein in patients whose smoking index≥400 and in patients that＜400 was 56.25% and 22.73%,respectively,the differences were significant(χ2=8.947,P=0.003).⑤WWOX and Bcl-2 protein expressions had no obvious relationship with ages,pathological type,degree of differentiation and clinical stage.⑥WWOX protein expression had negative correlation with Bcl-2 protein expression in lung cancr tissues.Conclusion WWOX protein expression in lung cancer was lower than that in adjacent normal lung tissue,Bcl-2 protein expression in lung cancer tissues was higher than that in adjacent normal lung tissue.WWOX protein expression had negative correlation with Bcl-2 protein expression in lung cancer tissues.

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备注/Memo

备注/Memo:
作者简介:柴春艳(1982-),女,硕士研究生,主治医师,主要从事呼吸内科疾病诊治,研究方向:肺癌的早期诊断和治疗研究,Tel:13669249528,E-mail:swallowchai 3412@163.com。
更新日期/Last Update: 2017-01-20