[1]容莉莉,关小珊,刘海英,等.广州地区GBS阳性孕妇GBS致病菌株的基因分型及分子流行病学调查[J].现代检验医学杂志,2017,32(01):87-90.[doi:10.3969/j.issn.1671-7414.2017.01.024]
　RONG Li-li,GUAN Xiao-shan,LIU Hai-ying,et al.Genotyping and Molecular Epidemiology Investigation of GBS Pathogenic Strains of GBS Positive Pregnant Women in Guangzhou[J].Journal of Modern Laboratory Medicine,2017,32(01):87-90.[doi:10.3969/j.issn.1671-7414.2017.01.024]
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广州地区GBS阳性孕妇GBS致病菌株的基因分型及分子流行病学调查()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
87-90
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Genotyping and Molecular Epidemiology Investigation of GBS Pathogenic Strains of GBS Positive Pregnant Women in Guangzhou
文章编号:
1671-7414(2017)01-087-04
作者:
容莉莉; 关小珊; 刘海英; 周珍文; 杨丽媛
广州市妇女儿童医疗中心检验科,广州 510120
Author(s):
RONG Li-li; GUAN Xiao-shan; LIU Hai-ying; ZHOU Zhen-wen; YANG Li-yuan
Department of Laboratory Medicine,Women and Children's MedicalCenter of Guangzhou City,Guangzhou 510120,China
关键词:
GBS致病菌株;  基因分型;  GBS阳性孕妇;  分子流行病学调查
分类号:
R378.12; R181.32
DOI:
10.3969/j.issn.1671-7414.2017.01.024
文献标志码:
A
摘要:
目的 研究探讨广州市GBS阳性孕妇GBS致病菌株的基因分型及其各种基因型的流行分布情况,为GBS致病菌株的快速分子诊断及其流行病学监测提供一定的理论依据和方法。方法 在广州市采用多阶段分层整体抽样的方法从2015年01月～12月就诊的GBS阳性孕妇的生殖道中收集的标本,药敏质控标准菌株:肺炎链球菌(ATCC49619)及金黄色葡萄球菌(ATCC25923),采取菌株培养、鉴定、DNA提取、PCR试验、基因检测等方法,通过相关软件进行相关数据分析,分析GBS致病菌株的基因分型及分子流行病学状况。结果 共收集分泌物标本2 812份,经菌株鉴定分离出178株GBS致病菌株,其检出率为6.33%。GBS致病菌株对利奈唑烷、万古霉素、青霉素、呋喃妥因等抗菌药物耐药率均为0,对氨苄青霉素、环丙沙星、莫西沙星及左旋氧氟沙星部分耐药,其耐药率分别为1.1%,16.9%18.0%及22.5%,而GBS致病菌株对红霉素、克林霉素等抗菌药物均出现了较高的耐药率,其耐药率分别为50.6%,47.8%(其中红霉素诱导克林霉素耐药有20例,占23.5%)和73.0%。65株GBS检测出mreA基因,56株GBS检测出ermB基因,36株GBS检测出mefA基因,28株GBS检测出mefE基因,5株GBS检测出ermA基因,ermC基因未被检测出。其中携带5种耐药基因的有3株(1.69%),携带4种耐药基因的有15株(8.43%),携带3种耐药基因的有19株(10.67%),携带2种耐药基因的有25株(14.04%),携带1种耐药基因的有5株(2.81%),不携带耐药基因的有1株(0.56%)。五种耐药基因的核苷酸序列,其同源性均为100%,无基因突变发生。结论 GBS致病株耐药基因主要是mreA,ermA,ermB,mrfA,mefE,且其核苷酸序列同源性为100%,临床上需加强耐药基因分子水平检测,指导临床更加合理科学的用药。
Abstract:
Objective To study genotyping and molecular epidemiology distribution of GBS pathogenic strains of GBS positive pregnant womenin Guangzhou,for GBS pathogenic strains of rapid molecular diagnosis and epidemiological surveillance provide certain theoretical basis and method.Methods In the Guangzhou area,used multi stage stratified samplingmethod collecting GBS positive pregnant women's reproductive tract specimens from January to December 2015,drug sensitivity quality control standard strains:Streptococcus pneumoniae(ATCC49619)and Staphylococcus aureus(ATCC25923),took culture of bacterial,strain,identification,DNA extraction,PCR,gene detection method,through the relevant software for data analysis,analyzedGBS strains of gene and molecular epidemiology.Results In the study,collected 2 812 samples of secretions,after identification of strains isolated from 178 strains of pathogenic GBS strains,the detection rate was6.33%.GBS pathogenic strains to linezolid vancomycin,penicillin,nitrfurantionand other antimicrobial drug resistance rate was 0,GBS parhogenic strains to ampicillin,ciprfloxacin moxifloxacin and levofloxacintesistant parts,the restancerates were 1.1%,16.9%,18.0% and 22.5%,but GBS pathogenic strains to erythromycin,clindamycin tetracydine antibiotics showed a high resistance rate,the resistance rates were 50.6%,47.8%(of which 20 cases of erythromycin induced clindamycin resistance accouted for 23.5%)and 73.0%.Among them,65 strains of GBS detected the mreA gene,56 strains of GBS detected the ermB gene,36 strainsof GBS detected the mefA gene,28 strains of GBS detected the mefE gene,5 strains of GBS detected the ermA gene,ermC gene was not detected in the gene.Amongthem,carried five multidrug resistance gene of 3 strains(1.69%)and 4 kinds of resistant gene carried with 15 strains(8.43%),carried three resistance genes of 19 strains(10.67%),2 kinds of resistant gene carrying a 25 strains(14.04%),carried the resistance gene of 5 strains(2.81%),did not carry resistance gene of 1 strain(0.56%).The nucleotide sequences of the five drug resistance genes were 100%,and no gene mutation occurred.Conclusion The main GBS disease resistant gene was mreA,ermA,ermB,mrfA,mefE and its nucleotide sequence homology was 100%.The clinical need to strengthen the detection of resistant gene and molecular level and guide clinical more scientific and rational drug use.

参考文献/References:

[1] 王 心,尚丽新.围生期B族链球菌感染诊治及预防新进展[J].医学综述,2016,22(3):530-532. Wang X,Shang LX.Researoh progress of diagnosis,treatment and prevention of perinatal group B streptococcal[J].Medical Review,2016,22(3):530-532.
[2] 郑海燕,温素珍,李文婷,等.韶关市2 821名孕妇围产期B族链球菌感染率调查及防治研究[J].中国初级卫生保健,2015,29(1):73-74,77. Zheng HY,Wen SZ,Li WT,et al.A investigation of 2 812 perinatal group B streptococcus infection rates and prevention research in Shaoguan[J].China Primary Health Care,2015,29(1):73-74,77.
[3] 陈惠玲,邓家德,叶慧芬,等.围产期生殖道感染B族溶血性链球菌的耐药性及耐药基因检测[J].中华妇产科杂志,2010,45(9):701-703. Chen HL,Deng JD,Ye HF,et al.Drug resistance and drug resistancegene detection of Streptococcus B in the perinatal genital tract infection[J].Chinese Journal of Obstetrics and Gynecology,2010,45(9):701-703.
[4] 张育娟,黄日妹,张炜灵.浅析B族链球菌的耐药性及红霉素与四环素耐药基因检测[J].海峡药学,2015,27(9):152-153. Zhang YJ,Huang RM,Zhang WL.Detection and drug resistance of erythromycin and tetracycline resistant gene of Streptococcus B[J].Strait Pharmaceutical,2015,27(9):152-153.
[5] 时春艳,赵扬玉,范 玲,等.实时聚合酶链反应技术检测妊娠晚期孕妇B族溶血性链球菌的多中心研究[J].中华围产医学杂志,2014,17(6):361-364. Shi CY,Zhao YY,Fan L,et al.A multi-center study on realtimepolymerase chain reaction assay for group B Streptococcus in pregnant women[J].Chinese Journal of Perinatal Medicine,2014,17(6):361-364.
[6] 王 萍.B族链球菌耐药[J].中华实用儿科临床杂志,2016,31(4):247-252. Wang P.Antibiotics resistance of group B Streptococcus[J].Chinese Journal of Practical Pediatrics,2016,31(4):247-252.
[7] 李亚梅,张利侠,秦 利,等.围产期孕妇B族链球菌的感染和耐药性检测及对妊娠结局的影响[J].现代检验医学杂志,2013,28(1):87-89. Li YM,Zhang LX,Qin L,et al.Detection of group B Streptococcus and analysis of drug resistance in perinatal pregant women and the influenceof thepregnancy outcome[J].Journal of Modern Laboratory Medicine,2013,28(1):87-89.
[8] 王彦春,何三军.汉中地区孕妇生殖道B族链球菌定植和防御素水平的相关性研究[J].现代检验医学杂志,2013,28(5):87-88,92. Wang YC,He SJ.Correlation between the colonization of group BStreptococcus and the level of defensins in pregnant women in Hanzhong[J].Journal of Modern Laboratory Medicine,2013,28(5):87-88,92.
[9] 何红美.2014年石家庄地区围产期妇女B族链球菌的耐药性及其耐药基因的研究[D].石家庄:河北医科大学,2014. He HM.Study on resistance and resistant genes of group B Streptococcus from perinatal women in Shijiazhuang during 2014[D].Shijinzhuang:Hebei Medical University,2014.
[10] 鲁 曦,金发光,徐冬旸,等.3种亚抑菌浓度抗生素对耐药基因水平传播的影响[J].中国抗生素杂志,2014,39(5),379-384. Lu X,Jin FG,Xu DY,et al.The effect of resistance genes horizontal transfer under stimulation of subinhibitory concentrations of antibiotics[J].Chinese Journal of Antibiotics,2014,39(5):379-384.

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备注/Memo

备注/Memo:
基金项目:广东省广州市医药卫生科技项目(项目编号:20161A010026)。
作者简介:容莉莉(1982-),女,本科,主管技师,专业:检验,E-mail:lilyrong@yeah.net。
更新日期/Last Update: 2017-01-20