[1]李腾达,谷明莉,邓顺江,等.热适应/热射病大鼠血清中microRNA let-7b的变化及意义[J].现代检验医学杂志,2017,32(02):19-22.[doi:10.3969/j.issn.1671-7414.2017.02.005]
　LI Teng-da,GU Ming-li,DENG Shun-jiang,et al.Change of MicroRNA let-7b in Serum of Rats with Heat Acclimatization/Heat Stroke and Its Significance[J].Journal of Modern Laboratory Medicine,2017,32(02):19-22.[doi:10.3969/j.issn.1671-7414.2017.02.005]
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热适应/热射病大鼠血清中microRNA let-7b的变化及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
19-22
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Change of MicroRNA let-7b in Serum of Rats with Heat Acclimatization/Heat Stroke and Its Significance
文章编号:
1671-7414(2017)02-019-04
作者:
李腾达1; 谷明莉1; 邓顺江1; 孙 懿1; 张薇薇1; 郭 杰1; 刘 云1; 黄元兰2; 钱 琤3; 邓安梅1
1.第二军医大学长海医院,上海 200433;
2.解放军455医院,上海 200052;
3.解放军100医院,江苏苏州 215007
Author(s):
LI Teng-da1; GU Ming-li1; DENG Shun-jiang1; SUN Yi1;  ZHANG Wei-wei1; GUO Jie1; LIU Yun1; HUANG Yuan-lan2; QIAN Cheng3; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433,China;
2.No.455 Hospital of PLA,Shanghai 200052,China;
3.No.100 Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
microRNA let-7b;  热射病;  热适应;  热休克蛋白;  肿痛坏死因子-α
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2017.02.005
文献标志码:
A
摘要:
目的 探究热适应/热射病大鼠模型血清中microRNA let-7b表达的变化及其与HSP70,IL-6,TNF-α,TGF-β和IL-12的相关性,分析其临床意义。方法 在第二军医大学实验动物中心选取肛温、体重、周龄基本一致的雄鼠60只随机均分为对照组、热适应组、热射病组,于仿真热气候动物舱中建立热适应/热射病大鼠模型后采集心尖血并分离血清。以实时荧光定量PCR(qRT-PCR)法检测血清中microRNA let-7b,以酶联免疫吸附法(ELISA)检测血清中HSP70,IL-6,TNF-α,TGF-β和IL-12的蛋白水平。三组间计量资料的比较采用Kruskal-Wallis H检验,以Spearman秩相关系数表示热射病组两变量之间的相关性。结果 miRNA let-7b在对照组、热适应组、热射病组的M分别为0.99,1.04和1.93,Q分别为0.30,0.25和0.44,差异具有统计学意义(χ2=38.95,P<0.001),组间两两比较结果显示let-7b在对照组与热适应组表达差异无统计学意义(P>0.05),在对照组与热射病组、热适应组与热射病组表达差异具有统计学意义(P<0.05); Spearman相关性分析显示热射病组let-7b与HSP70,TNF-α和IL-6呈正相关,差异具有统计学意义(rs=0.579,0.498和0.609,P<0.05)。结论 miRlet-7b可能参与了热射病病理过程,为临床监测高温湿环境下患者的状态提供了潜在的评价指标。
Abstract:
Objective To explore the change of microRNA let-7b in heat acclimatization/heat stroke rat models and its relation with HSP70,IL-6,TNF-α,TGF-β and IL-12,and analyze its clinical significance.Methods 60 male rats with almost the same anal temperature,weight,and weeks'age were selected from Laboratory Animal Center in the SecondMilitary Medical University.They were randomly divided into control,heat acclimatization,heat stroke groups averagely.Heat acclimatization/heat stroke ratmodels were built in hot climate simulated animal tank,blood was collected fromcordis apex and serum was separated.Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to test microRNA let-7b in serum,enzyme-linked immunosorbent reaction(ELISA)was to measure the protein level of HSP70,IL-6,TNF-α,TGF-β and IL-12 in serum.Kruskal-Wallis H test was usedto analyze quantitative data in the three groups,Spearman rank correlation coefficient was used to reveal relation between two variables in heat stroke group.Results M values of miRNA let-7b in control,heat acclimatization,heat stroke groups were 0.99,1.04 and 1.93 separately,Q valueswere 0.30,0.25 and 0.44(χ2=38.95,P<0.001),separately,with statistical significance.The results of pairwise comparison showed no statisticaldifference in control and heat acclimatization(P>0.05),but there were differences in control and heat stroke,heat acclimatization and heat stroke groupsstatistically(P<0.05).Spearman correlation analysis showed that in heatstroke group,let-7b was positively related with HSP70,TNF-α and IL-6(rs=0.579,0.498 and 0.609,P<0.05)with statistical significance.Conclusion miR let-7b might involve in the pathology of heat stroke,it provided a potential biomarker for monitoring patients in high temperature and humidity clinically.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,12MA056,15ZD009,15XD007),美捷登基金(MJR20150019)。
作者简介:李腾达(1990-),女,在读硕士研究生,主要从事感染免疫研究,Tel:18302192042,E-mail:tengdali@smmu.edu.cn。 谷明莉(1983-),女,检验技师,主要从事临床检验诊断工作,E-mail:mingligu@126.com,共同第一作者。
通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 钱 琤,女,主治医师,E-mail:qiancheng824@126.com,共同通讯作者。
更新日期/Last Update: 2017-04-10