[1]杨 柯a,郭晓宇b,欧剑锋a,等.外周血粒细胞CD55,CD59和FLAER检测在贫血及PNH诊断中的意义[J].现代检验医学杂志,2017,32(03):6-10.[doi:10.3969/j.issn.1671-7414.2017.03.002]
 YANG Kea,GUO Xiao-yub,OU Jian-fenga,et al.Diagnostic Significance of Detecting Peripheral Blood Granulocyte CD55/CD59 and FLAER in Anemia and PNH[J].Journal of Modern Laboratory Medicine,2017,32(03):6-10.[doi:10.3969/j.issn.1671-7414.2017.03.002]
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外周血粒细胞CD55,CD59和FLAER检测在贫血及PNH诊断中的意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
6-10
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Diagnostic Significance of Detecting Peripheral Blood Granulocyte CD55/CD59 and FLAER in Anemia and PNH
文章编号:
1671-7414 (2017)03-006-05
作者:
杨 柯a郭晓宇b欧剑锋a白 海a潘耀柱a
兰州军区兰州总医院a.血液科血液病研究所;
b.动物实验科,兰州 730050
Author(s):
YANG KeaGUO Xiao-yubOU Jian-fengaBAI Hai aPAN Yao-zhua
a.Department of Hematology;
b.Department of Animal Sci ence, Lanzhou General Hospital of Lanzhou Military Region,Lanzhou 730050,China
关键词:
CD55CD59嗜水气单胞菌毒素变异体贫血阵发性睡眠性 血红蛋白尿
分类号:
R556;R392.11
DOI:
10.3969/j.issn.1671-7414.2017.03.002
文献标志码:
A
摘要:
目的 探讨CD55,CD59和嗜水气单胞菌毒素变异体(FLAER) 检测在各类贫血及阵发性睡眠性血红蛋白尿症(PNH)患者诊断中的临床意义。方 法 选取2009年1月~2017年3月期间收集的30例健康对照,22例PNH,33例再生 障碍性贫血(AA),37例缺铁性贫血(IDA),45例巨幼细胞性贫血(MA),30例溶血性贫血(HA) 和31例骨髓增生异常综合征(MDS)患者,用多参数流式细胞仪同时检测外周血粒细胞CD55,C D59和FLAER阴性细胞比例,代表检出的PNH克隆数。结果 PNH,AA和 MDS组FLAER检出率均高于CD55和CD59,但只有AA患者差异有统计学意义(χ2=7.759,5 .518,P=0.005,0.019<0.05)。PNH和AA组CD55,CD59和FLAER阴性细胞比例均显著 高于正常对照组及其他贫血组(t=2.163~17.890,P=0.000~0.038<0.05)。PNH组F LAER阴性细胞比例高于CD59,CD59高于CD55,差异均具有统计学意义(t=2.503~6.308, P=0.000~0.016<0.05)。结论 CD55,CD59和FLAER在PNH诊断及 与 其他贫血性疾病鉴别诊断中具有重要价值,尤其是存在微小PNH克隆的MDS和AA患者,FLAER 优于CD59,CD59优于CD55。
Abstract:
Objective To investigate the clinical significa nce of CD55,CD59 and Aeromonas hydrophila toxin variant (FLAER) in the diagnos is of anemia and paroxysmal nocturnal hemoglobinuria (PNH).Methods Collected 30 healthy controls,22 cases of PNH,33 cases of aplastic anemia (AA),37 cases of iron deficiency anemia (IDA),45 cases of megaloblasti c anemia (MA),30 cases of hemolytic anemia (HA) and 31 cases of myelodysplastic syndrome (MDS) from January 2009 to March 2017,CD55,CD59 and FLAER negative c ell ratio of peripheral blood neutrophil of them were detected by multiparameter flow cytometry.Results The detection rates of FLAER in PNH,AA and MDS groups were higher than those of CD55 and CD59,but there was si gnificant difference in AA (χ2=7.759,5.518,P=0.005,0.019<0.05).T he average CD55,CD59 and FLAER deletion rate in PNH and AA group were significa ntly higher than those in normal control group and other groups (t=2.163~17. 890,P=0.000~0.038<0.05).The number of FLAER in PNH group was higher than CD59 and CD59 was higher than CD55 with the statistically significant difference (t=2.503~6.308,P=0.000~0.016<0.05).Conclusion 〖WT5”BZ 〗CD55,CD59 and FLAER have important value in the diagnosis of PNH and differen tial diagnosis with other anemia diseases,and can also be used to detect the pr esence of MDS and AA in patients with PNH.FLAER outperforms CD59,CD59 outperfo rms CD55.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81372132);全军实验动物专项基金(SYDW2016 010)。
作者简介:杨 柯(1989-),男,硕士,主要从事血液病流式检测 诊断,E-mail:172485374@qq.com。
通讯作者:白 海(1962-),男,主任医师,博士,E-mail:ba ihai98@hotmail.com。
更新日期/Last Update: 2017-06-25