[1]李腾达a,司 宇a,刘 鹏a,等.Gas6在免疫性血小板减少症患者中表达增高及临床意义[J].现代检验医学杂志,2017,32(03):37-39.[doi:10.3969/j.issn.1671-7414.2017.03.010]
 LI Teng-daa,SI Yua,LIU Penga,et al.Increased Expression of Gas6 in Patients with Immune Thrombocytopenia and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(03):37-39.[doi:10.3969/j.issn.1671-7414.2017.03.010]
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Gas6在免疫性血小板减少症患者中表达增高及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
37-39
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Increased Expression of Gas6 in Patients with Immune Thrombocytopenia and Its Clinical Significance
文章编号:
1671-7 414(2017)03-037-03
作者:
李腾达1a司 宇1a刘 鹏1a徐贵霞 1a谷明莉1b叶 辛2张薇薇1b黄元兰3钱 琤4邓安梅 1a
1.长海医院 a.临床实验中心;
b.检验科,上海 200433;
2.复旦大 学,上海 200433;
3.解放军455医院,上海 200052;
4.解放军100医院,江苏苏州 215007
Author(s):
LI Teng-da1aSI Yu1aLIU Peng1aX U Gui-xia1aGU Ming-li1bYE Xin2 ZHANG Wei-wei1bHUANG Yuan-lan3QIAN Cheng4DENG An-mei 1a
1a.Clinical Experiment Center;
1b.Department of Labora tory Diagnosis,Changhai Hospital, Shanghai 200433,China;
2.Fudan University,Shanghai 200433,China;
3.No .455 Hospital of PLA,Shanghai 200052,China;
4.No.100 Hospital of PLA,Jiangsu Suz
关键词:
免疫性血小板减少症生长捕获特异性蛋白6白细胞介素-4 干扰素-γ白细胞介素-17
分类号:
R558.2;R392.11
DOI:
10.3969/j.issn.1671-7414.2017.03.010
文献标志码:
A
摘要:
目的 探究Gas6在免疫性血小板减少症(ITP)患者中的表达 情况及其临床意义。方法 以2013年10月~2015年12月于上海长海医 院血液科确诊的35例ITP患者外周血作为实验组,以同期体检的35例健康个体外周血作为对 照组,两组样本分离血浆后以酶联免疫吸附法(ELISA)检测Gas6,IFN-α,IL-4,IFN-γ 和IL-17蛋白表达水平,组间比较采用两独立样本t检验,对Gas6与IFN-α等细胞因子 的关系进行Pearson相关性分析。结果 Gas6在实验组与对照组的表 达水平为27.28±7.56 ng/ml vs 20.51±5.39 ng/ml(t=4.314,P<0.0001);IFN -γ在实验组与对照组的表达水平为221.67±57.64 pg/ml vs 45.32±16.79 pg/ml( t=17.38,P<0.000 1);IL-4在实验组与对照组的表达水平为113.86±26.48 pg/m l vs 49.87±14.98 pg/ml(t=12.44,P<0.000 1);IL-17在实验组与对照组的表 达水平为168.96±47.88 pg/ml vs 109.56±28.97 pg/ml(t=6.28,P<0.000 1) ;IFN-α在实验组与对照组的表达水平为34.83±8.12 pg/ml vs 29.89±5.76 pg/ml (t=2.936,P=0.004 5),差异均具有统计学意义(P<0.05)。Pearson相关性分析 显示Gas6与IL-17,IL-4,IFN-γ呈正相关(r=0.564,0.486,0.449,P均<0.0 5),差异具有统计学意义,与IFN-α不具有相关性。结论 Gas6可 能通过影响Th细胞亚群细胞因子的分泌参与了ITP致病过程,是临床上治疗和预测该病的潜 在靶位点。
Abstract:
Objective To investigate the expression level o f Gas6 in patients with Immune Thrombocytopenia (ITP) and its clinical significa nce.Methods The experimental group was peripheral blood samples collected from 35 cases diagnosed with ITP in hematology department of C hanghai Hospital in Shanghai from October 2013 to December 2015.Control group w as peripheral blood from 35 healthy examined individuals at the same time.After separating plasm from the two group samples,the protein level of Gas6,IFN-α ,IL-4,IFN-γ and IL-17 were measured by Enzyme-linked immunosorbent assay (ELISA),comparison of expressional level of the two groups was measured by t test.Pearson correlation analysis was used to decide the relation between Ga s6 and cytokines such as IFN-α.Results The expression level of Gas6 in experimental and control groups was 27.28±7.56 ng/ml vs 20. 51±5.39 ng/ml (t=4.314,P<0.000 1);IFN-γ was 221.67±57.64 pg/ml vs 45.32±16.79 pg/ml (t=17.38,P<0.000 1);IL-4 was 113.86±26.48 pg/m l vs 49.87±14.98 pg/ml (t=12.44,P<0.000 1);IL-17 was 168.96±47.88 pg/ml vs 109.56±28.97 pg/ml (t=6.28,P<0.000 1);IFN-α was 34.83±8 .12 pg/ml vs 29.89±5.76 pg/ml (t=2.936,P=0.004 5),all with statistic al differences (P<0.05).Pearson analysis showed that Gas6 was positively r elated with IL-17,IL-4,IFN-γ (r=0.564,0.486,0.449,P<0.05) and t here was difference statistically,but Gas6 was not correlated with IFN-α.〖W T5”HZ〗Conclusion Gas6 may participate in the disease formation of ITP through affection on cytokines secreted by Th cell subsets,and it was the potential therapeutic and predicted target for this disease clinically.

参考文献/References:

[1] McKenzie CG,Guo L,Freedman J,et al.Cellular immune dysfunctio n in immune thrombocytopenia (ITP)[J].British Journal of Haematology,2013,1 63(1):10-23.
[2] Ye X,Zhang L,Wang H,et al.The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia[J].PloS One,2015,10(1):e0117704.
[3] Talaat RM,Elmaghraby AM,Barakat SS,et al.Alterations in immune cell s ubsets and their cytokine secretion profile in childhood idiopathic thrombocytop enic purpura (ITP)[J].Clinical and Experimental Immunology,2014,176(2):291 -300.
[4] Abdelsadik A,Trad A.Toll-like receptors on the fork roads between inna te and adaptive immunity[J].Human immunology,2011,72(12):1188-1193.
[5] van der Meer JH,van der Poll T,van’t Veer C.TAM receptors,Gas6,and protein S:roles in inflammation and hemostasis[J].Blood,2014,123(16):246 0-2469.
[6] Alciato F,Sainaghi PP,Sola D,et al.TNF-alpha,IL-6,and IL-1 expre ssion is inhibited by GAS6 in monocytes/macrophages[J].Journal of Leukocyte B iology,2010,87(5):869-875.
[7] Rothlin CV, Lemke G .TAM receptor signaling and autoimmune disease[J] . Current Opinion in Immunology,2010,22(6):740-746.
[8] Kim HA,Nam JY,Jeon JY,et al.Serum growth arrest-specific protein 6 l evels are a reliable biomarker of disease activity in systemic lupus erythematos us[J].Journal of Clinical Immunology,2013,33(1):143-150.
[9] Chen CH,Chen HC,Chang CC,et al.Growth arrest-specific 6 protein in p atients with sjogren syndrome:determination of the plasma level and expression in the labial salivary gland[J].PloS One,2015,10(10):e0139955.
[10] Sainaghi PP,Collimedaglia L,Alciato F,et al.Gro-wth arrest specific gene 6 protein concentration in cerebrospinal fluid correlates with rel apse severity in multiple sclerosis[J].Mediators Inflammation,2013(2013):4 06483.
[11] 中华医学会血液学分会血栓与止血学组.成人原发免疫性血小板减少症诊治的中国 专家共识(修订版)[J].中华血液学杂志,2011,32(3):214-216. Hemostasis and Thrombosis Group,Hematology Society,Chinese Medical Assoc iation.Expert consensus for diagnosis of adult primary immune thrombocytopenia in China (Revised Edition)[J].Chinese Journal of Hematology,2011,32(3):214 -216.
[12] 叶 辛,张 蕾,谷明莉,等.TRAIL在原发免疫性血小板减少症患者外周血单个 核细胞中的表达及临床意义[J].现代检验医学杂志,2014,29(5):46-48. Ye X,Zhang L,Gu ML,et al.Increased TRAIL expression in peripheral bloo d mononuclear cells from patients with primary immune thrombocytopenia and its c linical significance[J].J Mod Lab Med,2014,29(5):46-48.

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备注/Memo

备注/Memo:
基金 项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406 ,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353 ),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023 ,15ZD009,15XD007),美捷登基金(MJR20150019)。
作者简介:李腾达(1990-),女,在读硕士生,主要
更新日期/Last Update: 2017-06-25