[1]王 静,陈 葳,曾晓艳,等.不同标本来源铜绿假单胞菌对β-内酰胺酶耐药表型的差异性分析[J].现代检验医学杂志,2017,32(03):63-66，70.[doi:10.3969/j.issn.1671-7414.2017.03.017]
　WANG Jing,CHEN Wei,ZENG Xiao-yan,et al.Analysis of the Difference of β-Lactamase-Resistant Phenotypes by Pseudomonas Aeruginosa from Different Specimens[J].Journal of Modern Laboratory Medicine,2017,32(03):63-66，70.[doi:10.3969/j.issn.1671-7414.2017.03.017]
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不同标本来源铜绿假单胞菌对β-内酰胺酶耐药表型的差异性分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
63-66，70
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Analysis of the Difference of β-Lactamase-Resistant Phenotypes by Pseudomonas Aeruginosa from Different Specimens
文章编号:
1671- 7414(2017)03-063-05
作者:
王 静，陈 葳，曾晓艳，张 祎，雷金娥
1.西安交通大学第一附属医院检验科，西安 710061
Author(s):
WANG Jing，CHEN Wei，ZENG Xiao-yan，ZHANG Yi，LEI Jin -e
1.Department of Clinical Laboratory，the First Affiliated Hospital of Xi’an Jiaotong University，X i’an 710061，China
关键词:
铜绿假单胞菌; β-内酰胺酶耐药表型
分类号:
R378.991；R446.5
DOI:
10.3969/j.issn.1671-7414.2017.03.017
文献标志码:
A
摘要:
目的 比较临床不同来源铜绿假单胞菌对β-内酰胺酶耐药 表型差异性，为指导临床合理使用抗生素提供理论依据。方法 收集 西安交通大学第一附属医院2015年1月～12月从临床标本中分离的478株铜绿假单胞菌，通过 VITE-K2 Compact细菌鉴定及药敏分析高级专家系统获得β-内酰胺酶耐药表型，对其耐 药表型和相关抗生素的耐药性做差异性统计分析。结果 478株铜绿 假单胞菌以表型5和表型3为主。痰液、引流液、胆管胆汁标本来源铜绿假单胞菌均以表型5 为主，分别占31.08%，34.71%和38.46%，两两比较各组差异无统计学意义(χ2值 分别为3.893，4.071，5.595，P>0.05)。尿液、分泌物、全血标本来源铜绿假单胞 菌均以表型3为主，分别占34.88%，27.78%和45.45%，两两比较各组差异无统计学意 义(χ2值分别为6.654，9.956，9.852，P>0.05)。痰液、引流液、胆管胆汁 分别与尿液、分泌物、全血标本来源铜绿假单胞菌耐药表型分布两两比较差异有统计学意义 (χ2=15.056~22.050，P<0.05)。通过对不同标本来源铜绿假单胞菌中不同β-内酰胺酶耐药 表型抗生素耐药性比较：痰液标本来源中亚胺培南、美罗培南、哌拉西林、哌拉西林/他唑 巴坦差异有统计学意义(χ2=22.225~39.025，P<0.05)；肝胆外科仅头孢他啶和美罗培南差异 有统计学意义(χ2=21.890~22.872，P<0.05)。结论 不同标本来源铜 绿假单胞菌对β-内酰胺酶耐药表型的比较分析有差异性，为指导临床合理应用抗生素及铜 绿假单胞菌的医院感染控制提供了理论依据。
Abstract:
Objective To compare clinical Pseudomonas aer uginosa different sources of β-lactamase-resistant phenotype differences，a s to provide theoretical basis for guiding clinical rational use of antibiotics ．Methods Isolation of 478 strains of Pseudomonas aerug inosa were collected from clinical specimens in the First Affiliated Hospital of Xi’an Jiaotong University from January to December 2015，by VITEK 2 Compact bacteria identification and drug sensitivity analysis of advanced expert system for β-lactamase-resistant phenotype，statistical analysis of drug resistance phenotype and antibiotic resistance．Results 478 strains of Pseudomonas aeruginosa were mainly composed of phenotype 5 and phenotype 3．Sputum，drainage fluid and bile duct bile specimens of Pseudomonas aerugino sa were based on phenotype 5，accounted for 31.08%，34.71% and 38.46%．Mult iple comparison χ2 were 3.893，4.071 and 5.595，There was no statistic al difference between groups compare significance (P>0.05)．Urine，secretio ns and whole blood samples of Pseudomonas aeruginosa were phenotype 3，accou nted for 34.88%，27.78%，45.45%；Multiple comparison χ2 were 6.654，9 .956 and 9.852．There was no statistical difference between groups compare sig nificance (P>0.05)．Sputum，drainage of　liquid，bile duct bile and urine， secretion，whole blood specimens respectively source of Pseudomonas aeruginosa resistant phenotype distribution of two comparative difference was statistica lly significant (χ2=15.056~22.050，P<0.05)．Comparing the resistance of differe nt β-lactamase-resistant phenotypes in Pseudomonas aeruginosa　isolates f rom different sources：the sputum specimen source in imipenem，meropenem，pipera cillin and piperacillin/tazobactam had significant difference (χ2=22.225~39.025，P <0.05)．There was statistical significance in department of hepatobiliary sur gery only ceftazidime and meropenem differences (χ2=21.890~22.872，P<0.05)．〖WT 5”HZ〗Conclusion The phenotypic analysis of β-lactamase-resistan t phenotypes of Pseudomonas aeruginosa from different specimens was differen t，which provided a theoretical basis for guiding the clinical application of an tibiotics and the control of nosocomial infection of Pseudomonas aeruginosa ．

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备注/Memo

备注/Memo:

作者简介：王 静(1984-)，女，硕士，检验师，主要从事微生物研究，E -mail：997920314@qq.com。
通讯作者：陈 葳，女，科主任，博士生导师。
更新日期/Last Update: 2017-06-25