[1]廉江平,丰 航,方志远,等.EMIT与HPLC法测定肾移植术后患者体内霉酚酸血药浓度结果比较[J].现代检验医学杂志,2017,32(03):74-78.[doi:10.3969/j.issn.1671-7414.2017.03.020]
　LIAN Jiang-ping,FENG Hang,FANG Zhi-yuan,et al.Research on Results of Plasma Mycophenolic Acid Concentration Det ected by EMIT and HPLC in the Patients after Renal Transplantation[J].Journal of Modern Laboratory Medicine,2017,32(03):74-78.[doi:10.3969/j.issn.1671-7414.2017.03.020]
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EMIT与HPLC法测定肾移植术后患者体内霉酚酸血药浓度结果比较()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
74-78
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Research on Results of Plasma Mycophenolic Acid Concentration Det ected by EMIT and HPLC in the Patients after Renal Transplantation
文章编号:
1671-74 14(2017)03-074-05
作者:
廉江平; 丰 航; 方志远; 李小川
陕西省人民医院药 学部，西安 710068
Author(s):
LIAN Jiang-ping; FENG Hang; FANG Zhi-yuan; LI Xiao-c huan
Department of Pharmacy，Shaanxi Provincial People’s Hospital，X i’an 710068，China
关键词:
霉酚酸; 酶扩大免疫法; 高效液相色谱法; 一致性
分类号:
R699.2；R969.1
DOI:
10.3969/j.issn.1671-7414.2017.03.020
文献标志码:
A
摘要:
目的 采用Pearson相关分析和Bland-Altman图研究酶扩大 免疫法(EMIT)和高效液相色谱法(HPLC)检测霉酚酸(MPA)血药浓度的相关性和一致性。〖HT5 ”H〗方法 收集2014年10月～2015年12月95例采用霉酚酸治疗的肾移植患者血 样435份，采用两种方法测定同份样本中的MPA，并将测定结果分为两个浓度水平，在各浓度 水平的测定结果间用SPSS13.0进行配对t检验及Pearson相关分析；绘制Bland-Altman 一致性限度考察两种方法测定结果的一致性。结果 EMIT法测定结果 高于HPLC法，两者间存在具有统计学意义的正偏差(20.94%±14.42%，P<0.001)，两 方法在不同的浓度水平具有良好的相关关系(r>0.98)和一致性。结论 〖H T5”K〗EMIT法测定MPA血药浓度结果较HPLC法偏高，两种方法所得结果间具有良好的相关性 和一致性。在MPA治疗药物监测时应对两种方法建立不同的治疗窗范围。
Abstract:
Objective The correlation and agreement of myco phenolic acid (MPA) plasma concentrations that detected by enzyme extended immun oassay (EMIT) and high performance liquid chromatography (HPLC) were studied usi ng Pearson’s correlation and Bland-Altman plots．METHODS 435 plasma samples we re collected from 95 renal transplant patients who were treated with MPA from Oc tober 2014 to December 2015．The MPA plasma concentrations were simultaneously m easured by EMIT and HPLC respectively，and the results were divided into two le vels．Paired t test and Pearson’s correlation were performed using SPSS13. 0 to evaluate the relationships between the results in each level．The Bland-Al tman plot was used to assess the agreement of the results of two methods．〖WT5 ”HZ〗Results Higher concentrations were obtained with EMIT，there w as a significant positive bias of EMIT for MPA(20.94%±14.42%，P<0.001)． Pearson’s correlation analysis and Bland-Altman analysis showed that the resul ts from different methods presented good correlation (r>0.98) and agreement ．Conclusion The results of EMIT were higher than that of HPLC．There were good correlation and agreement between the two methods．The di fferences between EMIT and HPLC suggest that different therapeutic window should be set up when the two methods are used for MPA therapeutic drug monitoring．

参考文献/References:

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备注/Memo

备注/Memo:

作者简介：廉江平(1971-)，男，硕士，副主任药师，主要研究方向：临床药 学及体内药物分析，E-mail：ljp2075@163.com。
通讯作者：丰 航(1976-)，女，本科，主要研究方向：医院药学 ，E-mail：277312304@qq.com。
更新日期/Last Update: 2017-06-25