[1]程 莉,谭婷婷,魏红霞,等.临床分离的耐碳青霉烯肺炎克雷伯菌的耐药机制研究[J].现代检验医学杂志,2017,32(03):112-114.[doi:10.3969/j.issn.1671-7414.2017.03.030]
 CHENG Li,TAN Ting-ting,WEI Hong-xia,et al.Resistance Mechanisms of Clinical Isolated Carbapenem-Resistant Klebsiella Pneumoniae[J].Journal of Modern Laboratory Medicine,2017,32(03):112-114.[doi:10.3969/j.issn.1671-7414.2017.03.030]
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临床分离的耐碳青霉烯肺炎克雷伯菌的耐药机制研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
112-114
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Resistance Mechanisms of Clinical Isolated Carbapenem-Resistant Klebsiella Pneumoniae
文章编号:
1671- 7414(2017)03-112-03
作者:
程 莉谭婷婷魏红霞张 葵
南京大学医学院附属鼓楼医院检验科,南京 210008
Author(s):
CHENG LiTAN Ting-tingWEI Hong-xiaZHANG Kui
Department of Laboratory Medicine,the Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China
关键词:
碳青霉烯类抗菌药物肺炎克雷伯菌耐药机制
分类号:
R378.996;R446.5
DOI:
10.3969/j.issn.1671-7414.2017.03.030
文献标志码:
A
摘要:
目的 探究肺炎克雷伯菌对碳青霉烯类抗菌药物耐药的分子 机制。方法 通过Carba NP确证试验检测碳青霉烯酶表型;PCR扩增 碳青霉烯酶基因,质粒介导AmpC酶基因,超广谱β-内酰胺酶基因;采用多序列位点分型(M LST)对菌株进行遗传相关性分析。结果 50株耐碳青霉烯肺炎克雷伯 菌中,42株PCR扩增KPC-2阳性,1株NDM-1阳性,其余7株未检测到碳青霉烯酶基因;产KPC -2肺炎克雷伯菌相关耐药基因的携带率为:bla CTX-M 21.5%,bla SHV 42.9%,bla TE M 69.1%和bla DHA 4.8%;MLST结果显示42株KPC-2阳性菌株中,37株为ST11型。〖HT5” H〗结论 KPC-2的产生是肺炎克雷伯菌对碳青霉烯类抗菌药物耐药的主要机制 ,且该院存在着ST11 产KPC-2肺炎克雷伯菌的暴发流行。
Abstract:
Objective To explore the molecular mechanisms o f carbapenem-resistant Klebsiella pneumoniae.Methods Carba NP confirmatory test were used to detect carbapenemases.Carbapenemase g enes,ESBL genes and plasmid-mediated AmpC genes were amplified by polymerase c hain reaction (PCR).The genetic correlation analysis was carried out by using m ultiple sequence type (MLST).Results 42 out of the 50 ca rbapenem-resistant Klebsiella pneumoniae strains were KPC-2-positive stra ins,1 strain was positive for NDM-1,the other 7 strains were not detected for any carbapenemase genes.The percentages of KPC-2-positive Klebsiella pneum oniae with the bla CTX-M,bla SHV,bla TEM,bla DHA were 21.5%,42.9%,69 .1% and 4.8% respectively.The results of MLST showed that 37 out of 42 KPC-2 positive strains were ST11.Conclusion The production o f KPC-2 is the main mechanism of Klebsiella pneumoniae resistance to carbap enem and there is an outbreak of ST11 KPC-2 Klebsiella pneumoniae in this hospi tal.

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备注/Memo

备注/Memo:
基金项目: 南京市医学科技发展资金(ZKX13028)。
作者简介:程 莉(1990-),女,硕士,初级检验技师 ,主要研究方向:临床微生物学及耐药机制研究,Tel:15751866705,E-mail:chenglime d@163.com。
通讯作者:张 葵,主任技师,硕士研究生导师,Tel:13505151 066,E-mail:zhangkui6103@163.com。
更新日期/Last Update: 2017-06-25