[1]郭 燕,蔡 斌,段 勇,等.HBsAb阳性隐匿性乙型肝炎病毒感染者HBV PreS-S区基因突变研究[J].现代检验医学杂志,2017,32(04):16-20.[doi:10.3969/j.issn.1671-7414.2017.04.005]
　GUO Yan,CAI Bin,DUAN Yong,et al.Study on PreS-S Gene Mutation of HBV in Occult Hepatitis B Virus Infected Persons with Positive HBsAb[J].Journal of Modern Laboratory Medicine,2017,32(04):16-20.[doi:10.3969/j.issn.1671-7414.2017.04.005]
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HBsAb阳性隐匿性乙型肝炎病毒感染者HBV PreS-S区基因突变研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
16-20
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Study on PreS-S Gene Mutation of HBV in Occult Hepatitis B Virus Infected Persons with Positive HBsAb
文章编号:
1671-7414(2017)04-016-05
作者:
郭 燕; 蔡 斌; 段 勇; 景媛媛; 白 珉; 现红斌; 巩晗实
陕西省血液中心,西安 710061
Author(s):
GUO Yan; CAI Bin; DUAN Yong; JING Yuan-yuan; BAI Min; XIAN Hong-bin; GONG Han-shi
Shaanxi Blood Center,Xi'an 710061,China)Abstract:Objective To analyze the mutation of PreS-S region in occult hepatitis B virus(OHBV
关键词:
隐匿性乙肝病毒感染;  HBV PreS-S区;  基因变异
分类号:
R512.62; Q754
DOI:
10.3969/j.issn.1671-7414.2017.04.005
文献标志码:
A
摘要:
目的 分析血清HBsAb阳性的隐匿性乙型肝炎病毒感染者HBV PreS-S区基因变异,初步探讨该特殊感染模式的发生机制。方法 使用巢式聚合酶链反应(Nested PCR),对陕西省血液中心保存的38份HBsAb阳性隐匿性乙肝病毒感染(OBI)血清样本进行HBV PreS- S基因片段扩增并测序,确定基因型和血清型,并与对应基因型HBsAg阳性野毒株序列进行比对。结果 38份HBsAb阳性OBI血清样本中,11份成功扩增出目的基因,其中8份测序成功。C基因型5份,其中1份为血清型adw,4份为血清型adr; B基因型3份,全部为血清型adw。OBI毒株PreS- S区,PreS- S免疫反应区以及亲水区(MHR)氨基酸变异率均显著高于对应野毒株,差异有统计学意义(2.6% vs 0.8%,χ2=40.23; 3.2% vs 0.3%,χ2=52.13; 3.6% vs 0.6%,χ2=13.25,均P<0.01),“α”抗原决定簇检测到I126T,Q129R,M133T,F134I,D144E,G145K突变。C基因型PreS-S区氨基酸变异率高于B基因型,差异有统计学意义(3.5% vs 1.2%,χ2=15.98,P<0.01); 而其MHR,“α”抗原决定簇以及 PreS-S免疫反应区氨基酸突变率虽均高于B基因型,但差异均无统计学意义(分别是4.7% vs 1.7%,χ2=2.96; 3.6% vs 2.9%,χ2=0.25; 4.1% vs 2.3%,χ2=3.59,均P>0.05)。结论 OBI毒株PreS-S区,尤其是PreS-S免疫反应区氨基酸发生较多变异,可能改变了病毒的免疫原性,造成免疫逃逸,从而导致HBsAb阳性OBI发生。
Abstract:
in HBV infected persons with positive HBsAb and investigate the biological mechanisms of the special infectious model.Methods A total of 38 HBsAb positive OBI serum samples wereamplified by Nested PCR and sequenced,HBV genotype and serotype were determined.The amino acid sequences of OHBV were compared to the corresponding sequenceof wild-type strains of similar genotype obtained from the GenBank database.Results PreS-S segment of 11 samples were obtained and 8 samples were sequenced successfully.Among which,5 were genotype C and 3 were genotype B.Genotype B were all serotype adw,while genotype C were 1 adw and 4 adr.The mutation rates of PreS-S region,the immunoreactive area and the major hydrophilic region(MHR)were higher in OHBV than the wild-type strains(2.6% vs 0.8%,χ2=40.23,3.2% vs 0.3%,χ2=52.13,3.6% vs 0.6%,χ2=13.25,all P<0.01)and the substitutions of I126T,Q129R,M133T,F134I,D144E,G145K in “α” determinant were found in OBI samples.The mutation rate of amino acids in PreS-S region was higher in genotype C than genotype B(3.5% vs 1.2%,χ2=15.98,P<0.01),meanwhile,the mutation rates in MHR,“α” determinant and immunoreactive region were higher in genotype C too,but no statistical significance was attained(4.7% vs 1.7%,χ2=2.96,3.6% vs 2.9%,χ2=0.25,4.1% vs 2.3%,χ2=3.59,allP >0.05).Conclusion Mutations in PreS-S region,especially in immunoepitope,might change the virus'immunogenicity leading to escape from immune response and cause OBI with HBsAb positive.

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备注/Memo

备注/Memo:
作者简介:郭 燕(1980-),女,博士研究生,研究方向:血液输血安全,E-mail:20524988@qq.com。 通讯作者:巩晗实(1985-),女,博士研究生,研究方向:血液输血安全,E-mail:hanshi-gong@163.com。
更新日期/Last Update: 1900-01-01