[1]胡 伟,王 森,黄太宏,等.双阴性调节性T细胞(DN Tregs在系统性红斑狼疮患者外周血中的变化及意义[J].现代检验医学杂志,2017,32(04):25-28.[doi:10.3969/j.issn.1671-7414.2017.04.007]
　HU Wei,WANG Sen,HUANG Tai-hong,et al.Change and Clinical Significance of Peripheral Blood DN Tregs in Patients with Systemic Lupus Erythematosus[J].Journal of Modern Laboratory Medicine,2017,32(04):25-28.[doi:10.3969/j.issn.1671-7414.2017.04.007]
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双阴性调节性T细胞(DN Tregs在系统性红斑狼疮患者外周血中的变化及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
25-28
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Change and Clinical Significance of Peripheral Blood DN Tregs in Patients with Systemic Lupus Erythematosus
文章编号:
1671-7414(2017)04-025-04
作者:
胡 伟; 王 森; 黄太宏; 徐学静; 夏永泉
南京大学医学院附属鼓楼医院检验科,南京 210008
Author(s):
HU Wei; WANG Sen; HUANG Tai-hong; XU Xue-jing; XIA Yong-quan
Department of Clinical Laboratory,Drum Tower Hospital Affiliated to Nanjing University Medical School,Nanjing 210008,China
关键词:
系统性红斑狼疮;  调节性T细胞;  CD4/CD8双阴性T细胞
分类号:
R593.241; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.04.007
文献标志码:
A
摘要:
目的 研究系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血中TCRαβ+CD3+CD4-CD8-T细胞即双阴性调节性T细胞(doublenegative regulatory T cells,DN Tregs)的表达水平及功能,并探讨DN Tregs在SLE疾病进程中的作用。方法 通过流式细胞术检测20例SLE患者及20例健康对照者外周血CD3+TCRαβ+细胞中DN Tregs细胞的比例; 采用ELISA的方法检测SLE患者及健康对照者血浆中IFN-γ,TNF-α,IL-6和IL-17A的表达水平,并分析DN Tregs与各细胞因子的相关性。结果 与健康对照组相比,SLE患者组DN Tregs占TCRαβ+细胞的比例升高,两组之间差异有统计学意义(t=3.54,P<0.01),SLE患者血浆中IFN-γ,TNF-α和L-6水平高于对照组,两组之间差异均具有统计学意义(t=2.824,2.085,2.304,均P<0.05)。DN Tregs的水平与IFN-γ的含量呈正相关(r=0.52,P=0.02),而与TNF-α,IL-6的含量无相关性(r值分别为0.17和0.16,P值分别为0.47 和0.49)。结论 SLE患者外周血DN Tregs细胞比例显著上升,DN Tregs细胞可能在SLE发病及进展中发挥重要作用。
Abstract:
Objective To study the expression and functionof TCRαβ+CD3+CD4-CD8- T(Double negative regulatory T cells,DN Tregs)in peripheral blood of Systemic lupus erythematosus(SLE)patients,and investigate their function in pathogenesis of SLE.Methods TCRαβ+CD4-CD8- T cells were quantified as percentage of total CD3+T cells in peripheral blood from 20 SLE patients and 20 healthy controls by flow cytometry.Used ELISA to test the cytokine levels(IFN-γ,TNF-α,IL-6,IL-17A)in the plasma of SLE patients.And analyzed the relationship between the percentage of DN Tregs and cytokines levels.Results The ratioof DN Tregs in TCRαβ+T cells was significantly increased in SLE patients compared to healthy donors(t=3.54,P<0.01).The levels of IFN-γ,TNF-α and IL-6in plasma of SLE patients were higher than healthy donors(t=2.824,2.085,2.304,P<0.05).The frequency of DN Tregs was found to correlate with IFN-γ(r=0.52,P=0.02)but not TNF-α(r=0.17,P=0.16)and IL-6(r=0.47,P=0.49).Conclusion This study reveals frequency of DN Tregs in peripheral blood of SLE patients was higher than healthy controls,the frequency of DN Tregs was also found to correlate with IFN-γ levels,which means that DN Tregs may play an important role in pathogenesis of SLE.

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备注/Memo

备注/Memo:
基金项目:国家自然基金青年基金资助(81601765)。 作者简介:胡 伟(1983-),女,硕士,主管技师,主要从事免疫学研究,E-mail:yf523180@163.com。 通讯作者:夏永泉,男,副主任技师,E-mail:xyq2002xyq@aliyun.com。
更新日期/Last Update: 1900-01-01