[1]杨 维,彭 晶,何秋仙,等.儿童恶性疟疾患者外周血基因表达谱的改变及生物信息学特征分析[J].现代检验医学杂志,2017,32(04):36-39.[doi:10.3969/j.issn.1671-7414.2017.04.010]
　YANG Wei,PENG Jing,HE Qiu-xian,et al.Changes in Gene Expression Profile and Bioinformatics Analysis of Children with Severe Malaria[J].Journal of Modern Laboratory Medicine,2017,32(04):36-39.[doi:10.3969/j.issn.1671-7414.2017.04.010]
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儿童恶性疟疾患者外周血基因表达谱的改变及生物信息学特征分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
36-39
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Changes in Gene Expression Profile and Bioinformatics Analysis of Children with Severe Malaria
文章编号:
1671-7414(2017)04-036-04
作者:
杨 维1; 彭 晶1; 何秋仙2; 张荣强3
1.西安市中医医院检验科,西安 710021; 2.陕西省人民医院体检中心,西安 710068; 3.陕西中医药大学公共卫生学院,陕西咸阳 712046
Author(s):
YANG Wei1; PENG Jing1; HE Qiu-xian2; ZHANG Rong-qiang3
1.Department of Clinical Laboratory,Xi'an Hospital of Traditional Chinese Medicine,Xi'an 710021,China; 2.Medical Examination Center,Shaanxi Provincial People's Hospital,Xi'an 710068,China; 3.School of Public Health,Shaanxi University of Traditional Chinese Medicine,Shaanxi Xianyang 712046,China
关键词:
疟原虫;  疟疾;  差异表达基因;  生物信息学
分类号:
R725.3; Q786
DOI:
10.3969/j.issn.1671-7414.2017.04.010
文献标志码:
A
摘要:
目的 分析儿童恶性疟疾患者外周血基因表达谱、关键蛋白的改变及其生理学功能,为疟疾防治提供新依据。方法 从基因芯片公共数据库GEO中获得疟疾患儿外周血基因芯片数据,共5例疟疾患儿,均依据WHO疟疾诊断标准明确诊断,采用自体匹配设计,每位患儿分别在轻症、重症疟疾时采集外周血; 分析不同病情患儿的基因表达谱、蛋白相互作用网络、分子生物学过程、基因功能,寻找疟疾患儿病情进展相关的关键基因。结果 623个(1.93%)差异表达基因可鉴别区分轻症、重症患儿; OAS2,OAS3,IFIT3和USP18基因是差异基因蛋白-蛋白交互作用网络(protein-protein interactions,PPI)中子网络的核心节点; 差异表达基因主要参与机体的免疫防御、免疫应答、对外部刺激应答、干扰素-1型通路的活化等生物学功能。结论 疟疾患儿病情的进展可能受OAS2,OAS3,IFIT3和USP18等基因的调控,儿童的免疫防御能力下降与病情进展具有重要的关系。
Abstract:
Objective To conduct bioinformatics analysis ofchildren with severe malaria to find out the key gene changes in order to provide a new basis for the prevention and treatment of malignant malaria.Methods Microarray gene chip data was downloaded from public databases GEO and imported into the analysis software STRING,PANTHER and GenClip.The gene expression profiles,protein interaction networks,the process of molecular biology,gene function were analyzed.Results 623(1.93%)differentially expressed genes had a good diagnostic capabilities in the diagnosis of mild and severe malaria.OAS2,OAS3,IFIT3 and USP18 were the core sub-network node of the Protein-Protein Interactions.Differentially expressed genes mainly involved in the body's immune defense,immune response,response toexternal stimuli,the biological function of type 1 interferon activation pathways.Conclusion The progress of malaria of children may be in the regulation of OAS2,OAS3,IFIT3,USP18 and children's immune defense capacity decreased,the malaria began to progress more easily.

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备注/Memo

备注/Memo:
基金项目:陕西中医药大学校内基金资助(基金编号:2015QN05)。 作者简介:杨 维(1981-),女,硕士,主管检验师,主要从事临床检验,Tel:15332408186,E-mail:95250473@qq.com。 通讯作者:张荣强,男,博士在读,Tel:18165311997,E-mail:zhangrqxianyang@163.com。
更新日期/Last Update: 1900-01-01