[1]朱 超,姜彦峰.股骨头坏死患者血清miRNA-210,miRNA-23b检测的应用价值[J].现代检验医学杂志,2017,32(04):57-59,151.[doi:10.3969/j.issn.1671-7414.2017.04.016]
　ZHU Chao,JIANG Yan-feng.Application Value of Detecting Serum Micrornas-210, miRNA-23b in the Patients with the Femoral Head Necrosis[J].Journal of Modern Laboratory Medicine,2017,32(04):57-59,151.[doi:10.3969/j.issn.1671-7414.2017.04.016]
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股骨头坏死患者血清miRNA-210,miRNA-23b检测的应用价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
57-59,151
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Application Value of Detecting Serum Micrornas-210, miRNA-23b in the Patients with the Femoral Head Necrosis
文章编号:
1671-7414(2017)04-057-04
作者:
朱 超1; 姜彦峰2
1.陕西中医药大学第一附属医院骨三科,陕西咸阳 712000; 2.咸阳市中心医院检验科,陕西咸阳 712000
Author(s):
ZHU Chao1; JIANG Yan-feng2
1.the Third Departerment of Orthopaedics,the First Affiliated Hospitalof Shaanxi University of Traditional Chinese Medicine,Shaanxi Xianyang 712000,China; 2.Departerment of Clinical Laboratory,the Central Hospital of Xianyang,Shaanxi Xianyang 712000,China
关键词:
股骨颈骨折复位内固定术;  股骨头坏死;  miRNA-210;  miRNA-23b
分类号:
R687.4; Q503
DOI:
10.3969/j.issn.1671-7414.2017.04.016
文献标志码:
A
摘要:
目的 探讨血清微小RNA(microRNA,miRNA)-210,-23b检测在股骨头坏死患者中的临床应用价值。方法 收集2015年1月～2017年1月于陕西中医药大学第一附属医院骨三科住院行股骨颈骨折内固定手术治疗的135例患者的血清及临床资料,依据是否发生股骨头坏死分为坏死组和非坏死组,其中坏死组27例,非坏死组108例。采用实时荧光定量PCR(Real time-PCR,RT-PCR)技术检测血清miRNA-210,miRNA-23b的表达。比较分析血清miRNA-210,miRNA-23b的变化与股骨头坏死之间的关系。结果 坏死组血清miRNA-210,miRNA-23b表达分别为(0.19±0.03,0.21±0.05); 非坏死组血清miRNA-210,miRNA-23b表达分别为(0.92±0.18,0.87±0.17)。坏死组血清miRNA-210,miRNA-23b表达分别与非坏死组比较显著下调,其差异均有统计学意义(t=35.29,31.20,P<0.01)。采用受试者工作特征曲线(ROC曲线)分析血清miRNA-23b,miRNA-210的AUC分别为0.790(95%CI:0.687～0.892,P=0.000)和0.743(95% CI:0.633～0.854,P=0.000)。miRNA-23b,miRNA-210在最佳临界值处诊断股骨头坏死的敏感度和特异度分别为95.7%和93.9%,91.5%和92.3%。结果显示血清miRNA-23b,miRNA-210的表达对诊断股骨头坏死的发生有临床意义。结论 检测血清miRNA-210,miRNA-23b表达可预测股骨头坏死的发生,估计病情的发展状况。
Abstract:
Objective To investigate the application valueof detection of serum miRNA-210,miRNA-23b in the patients with the femoral head necrosis.Methods The serum and clinical data of 135 patients with the internal fixation operation for the femoral neck fracture werecollected.The patients were divided into the necrosis group(27 cases)and non-necrosis group(108 cases),used the real-time fluorescent quantitative PCR(Real time-PCR,RT-PCR)technology to detect serum miRNA-210,miRNA-23b expression in comparatively analysizing the relationship between the changes of serummiRNA-210,23b and occurrence of the femoral head necrosis.ResultsThe serum microRNA-210,-23b expression in the necrosis group were 0.19±0.03,0.21±0.05,which in the non-necrosis group were 0.92±0.18and 0.87±0.17.The serum microRNA-210,-23b expression in the necrosis group were significantly lower than those in the non-necrosis group,the difference had statistical significance(P<0.01).The analysis results from the receiver-operating characteristic curve(ROC curve)showed that AUC of serum miRNA-210,miRNA-23b expression were 0.790(95%CI:0.687～0.892,P=0.000)and 0.743(95%CI:0.633～0.854,P=0.000),respectively.The sensitivityand specificity of miRNA-210 and miRNA-23b at the best threshold in diagnosisof the femoral head necrosis were 95.7% and 93.9%,91.5% and 92.3%,respectively.The analysis results showed that the serum miRNA-210,-23b expression had clinical significance in diagnosis of the femoral head necrosis.Conclusion Detection of serum miRNA-210,-23b expression can predict occurrence of the femoral head necrosis, estimate development condition ofthe desease.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:朱 超(1975-),男,硕士研究生,副主任医师,研究方向:股骨头坏死的诊断与治疗,E-mail:280310216@qq.com。 通讯作者:姜彦峰(1973-),男,主管检验师,研究方向:疾病的基因诊断,E-mail:13892998280@163.com。
更新日期/Last Update: 1900-01-01