[1]李 兵a,傅 尧a,符义富a,等.口腔鳞状细胞癌患者血清中CCL2和VEGF表达的初步研究[J].现代检验医学杂志,2017,32(04):64-66,71.[doi:10.3969/j.issn.1671-7414.2017.04.018]
　LI Binga,FU Yaoa,FU Yi-fua,et al.Preliminary Study on the Expression of CCL2 and VEGF in Serum of Patients with Oral Squamous Cell Carcinoma[J].Journal of Modern Laboratory Medicine,2017,32(04):64-66,71.[doi:10.3969/j.issn.1671-7414.2017.04.018]
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口腔鳞状细胞癌患者血清中CCL2和VEGF表达的初步研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
64-66,71
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Preliminary Study on the Expression of CCL2 and VEGF in Serum of Patients with Oral Squamous Cell Carcinoma
文章编号:
1671-7414(2017)04-064-04
作者:
李 兵a; 傅 尧a; 符义富a; 游丽萍a; 刘 畅a; 孙国文b
南京大学医学院附属口腔医院 a.检验科; b.口腔颌面外科,南京210008
Author(s):
LI Binga; FU Yaoa; FU Yi-fua; YOU Li-pinga; LIU Changa; SUN Guo-wenb
a.Department of Clinical Laboratory; b.Oral and Maxillofacial Surgery,Stomatology Hospital Affiliated to Medical College of Nanjing University,Nanjing 210008,China
关键词:
口腔鳞状细胞癌;  单核细胞趋化蛋白-1;  血管内皮生长因子;  血清;  筛查指标
分类号:
R739.8; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.018
文献标志码:
A
摘要:
目的 探讨口腔鳞状细胞癌(OSCC)患者血清中单核细胞趋化蛋白-1(MCP-1/CCL2)及血管内皮生长因子(VEGF)的表达及意义。方法 收集27例健康对照者和85例OSCC患者的血清,用ELISA方法检测CCL2及VEGF的表达水平,分析两者相关性及与临床病理参数的关系。结果 OSCC患者血清CCL2水平为69.12±19.54 pg/ml,显著低于健康对照者(103.41±34.42 pg/ml),差异具有统计学意义(t=6.477,P<0.05)。OSCC患者血清CCL2水平与TNM分期明显相关(t=2.193,P<0.05)。OSCC患者血清VEGF水平为145.76±49.34 pg/ml,显著高于健康对照者(70.35±14.93 pg/ml),差异具有统计学意义(t=3.92,P<0.05)。CCL2水平与VEGF水平呈负相关关系(r=-0.216,P<0.05)。受试者工作特征曲线(ROC曲线)显示血清中CCL2水平及CCL2/VEGF比值都能够很好地区分健康人和OSCC患者(P<0.05),其最佳临界值分别为98.61 pg/ml及0.82。结论 OSCC患者血清中CCL2表达降低,VEGF表达增高,提示其可能与OSCC发生、发展相关。检测血清CCL2及VEGF水平可以作为OSCC的筛查指标。
Abstract:
Objective To study the expression and significance of monocyte chemotactic protein-1(MCP-1/CCL2)and vascular endothelial growth factor(VEGF)in serum samples of oral squamous cell carcinoma(OSCC)patients.Methods The concentrations of CCL2 and VEGF in the serum was assessed by ELISA in healthy donors(n=27)and OSCC patients(n=85).Then analyzed the correlation between the concentrations of CCL2 and VEGF and the relationship with patients' clinicopathological characteristics.Results CCL2 concentration was lower in OSCCpatients than in healthy donors(69.12±19.54 pg/ml vs 103.41±34.42 pg/ml,t=6.477,P<0.05).The expression of CCL2 was positively associated to TNM stage in OSCC(t=2.193,P<0.05).VEGF concentration was higher in OSCC patients than in healthy donors(145.76±49.34 pg/ml vs 70.35±14.93 pg/ml,t=3.92,P<0.05).There was a negative correlation between CCL2 and VEGF(r=-0.216,P<0.05).The receiver operating characteristic(ROC)curve suggests that CCL2 and CCL2/VEGF in serum are good diagnostic markers to discriminatehealthy people from OSCC patients,the cutoff values was 98.61 pg/ml and 0.82.Conclusion The expression of CCL2 and VEGF in serum correlated to OSCC progression,and it can be a potential diagnostic biomarker fororal disease.

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备注/Memo

备注/Memo:
基金项目:江苏省卫生厅课题项目(H201441),南京市科技发展计划项目(201503038),南京市医学科技发展基金(ykk13150)。 作者简介:李 兵(1986-),男,硕士,主管技师,主要从事临床免疫学检验,E-mail:bluk222@163.com。 通讯作者:孙国文,男,副教授,Tel:025-83620330。
更新日期/Last Update: 1900-01-01