[1]王福玲.血清miR-122,ALT及HBV-DNA定量检测对慢性乙型肝炎患者诊断的意义[J].现代检验医学杂志,2017,32(04):67-71.[doi:10.3969/j.issn.1671-7414.2017.04.019]
　WANG Fu-ling.Diagnostic Value of Serum miR-122,ALT and HBV-DNA in Patients with Chronic Hepatitis B[J].Journal of Modern Laboratory Medicine,2017,32(04):67-71.[doi:10.3969/j.issn.1671-7414.2017.04.019]
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血清miR-122,ALT及HBV-DNA定量检测对慢性乙型肝炎患者诊断的意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
67-71
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Diagnostic Value of Serum miR-122,ALT and HBV-DNA in Patients with Chronic Hepatitis B
文章编号:
1671-7414(2017)04-067-05
作者:
王福玲
枣庄矿业集团中心医院检验科,山东枣庄 277800
Author(s):
WANG Fu-ling
Department of Clinical Laboratory,Central Hospital of Zaozhuang Mining Group, Shandong Zaozhuang 277800,China
关键词:
慢性乙型肝炎;  血清miR-122;  丙氨酸氨基转移酶;  乙肝病毒脱氧核糖核酸
分类号:
R512.62; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.04.019
文献标志码:
A
摘要:
目的 探究慢性乙型肝炎(CHB)患者血清miR-122和丙氨酸氨基转移酶(alanine aminotransferase,ALT)与乙肝病毒脱氧核糖核酸(hepatis B virusdeoxyribonucleic acid,HBV-DNA)的相关性,为慢性乙型肝炎患者的临床诊断提供参考。方法 选取枣庄矿业集团中心医院2014年1月～2016年1月收治的900例慢性乙型肝炎患者,检测其血清miR-122,ALT和HBV-DNA水平,并做三者与慢性乙型肝炎的相关分析。结果 乙肝血清标志物检测显示慢性乙肝或早期肝炎感染患者HBV-DNA载量显著高于其它患者(F=12.355,P=0.000),慢性乙型肝炎患者ALT含量显著高于其他患者(F=24.654,P=0.000),ALT和HBV-DNA相关性与HBV-M模式无显著关系; 在一定范围内,随着HBV-DNA载量的增加,ALT含量>80U/L的慢性乙型肝炎患者比例逐渐升高(u=86.313,P=0.000)。相关性分析表明,ALT含量与HBV-DNA载量显著正相关(r=0.919,P=0.034)。随着HBV-DNA载量的增加,慢性乙型肝炎患者血清miR-122水平逐渐升高(F=1463.435,P=0.000)。相关性分析表明,血清miR-122与HBV-DNA载量呈显著正相关(r=0.975,P=0.012)。结论 慢性乙型肝炎患者血清miR-122,ALT和HBV-DNA之间存在一定的相关性,三者在慢性乙型肝炎的诊断中具有良好的敏感度和特异度,因此在慢性乙型肝炎的诊断中应用三者联合检测,相互补充,以提高诊断准确性,帮助患者更好地进行治疗。
Abstract:
Objective To explore the clinical significanceof serum miR-122,ALT and HBV-DNA in patients with chronic hepatitis B(CHB).Methods 900 patients of CHB were selected in Zaozhuang Mining Group Hospital from January 2014 to January 2016,and theserum levels of miR-122,ALT and HBV-DNA were detected,and analysed the relevance among the three and chronic hepatitis B.Results Serum markers of hepatitis B test showed that the HBV-DNA load in patients with chronic hepatitis B or early hepatitis infection was significantly higher than that of other patients(F=12.355,P=0.000),and the ALT content in patientswith CHB was significantly higher than that of other patients(F=24.654,P=0.000).There was no significant correlation between ALT and HBV-DNA and HBV-M pattern.With the increase of HBV load,the proportion of hepatitis B patients with ALT >80 U/L increased gradually within limits(P<0.05).Correlation analysis showed that ALT content was positively correlated with HBV loading(r=0.919,P=0.034).With the increase of HBV load,the level of serum miR-122 in patientswith CHB was gradually increased(F=1463.435,P=0.000).Correlation analysis showed that serum miR-122 was positively correlated with HBV(r=0.975,P=0.012).Conclusion There was a correlationbetween serum miR-122,ALT and HBV-DNA in patients with CHB.The three indicators have good sensitivity and specificity in the diagnosis of CHB,socan use the three joint detection in the diagnosis in patients CHB,in order to improve the diagnostic accuracy and help patients better treatment.

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备注/Memo

备注/Memo:
作者简介:王福玲(1980-),女,本科,主管技师,研究方向:医学检验,E-mail:soy 551@163.com。
更新日期/Last Update: 1900-01-01