[1]徐瑞芬,赵清侠,陈 欣,等.载BSA壳聚糖纳米纤维体外缓释的研究[J].现代检验医学杂志,2017,32(04):119-121,124.[doi:10.3969/j.issn.1671-7414.2017.04.034]
　XU Rui-fen,ZHAO Qing-xia,CHEN Xin,et al.Study on the Sustained Release of BSA from Chitosan-OREC/BSA Films Coated Mats in Vitro[J].Journal of Modern Laboratory Medicine,2017,32(04):119-121,124.[doi:10.3969/j.issn.1671-7414.2017.04.034]
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载BSA壳聚糖纳米纤维体外缓释的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
119-121,124
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Study on the Sustained Release of BSA from Chitosan-OREC/BSA Films Coated Mats in Vitro
文章编号:
1671-7414(2017)04-119-04
作者:
徐瑞芬1; 赵清侠1; 陈 欣1; 郑星星1; 冯旭阳2
1.陕西省人民医院麻醉科,西安 710068; 2.第四军医大学附属西京医院心脏内科,西安 710032
Author(s):
XU Rui-fen1; ZHAO Qing-xia1; CHEN Xin1; ZHENGXing-xing1; FENG Xu-yang2
1.Department of Anesthesia,Shaanxi Provincial People's Hospital,Xi'an 710068,China; 2.Department of Cardiology,Xijing Hospital of Fourth Military Medical University, Xi'an 710032,China
关键词:
壳聚糖;  纳米纤维;  缓释
分类号:
R318.08
DOI:
10.3969/j.issn.1671-7414.2017.04.034
文献标志码:
A
摘要:
目的 探讨体外不同因素对载BSA壳聚糖纳米纤维缓释的影响。方法 利用层层自组装技术,制备壳聚糖及壳聚糖-有机累托石纳米纤维,以BSA为模型蛋白,探讨pH值、自组装层数及加入有机累托石对复合材料药物缓释的影响。结果 最初1 h只有少量的BSA释放,8 h达到最高峰。在相同的自组装层数以及测试时间点,PBS溶液中BSA的缓释量要多于盐酸溶液中的释放量。pH1.2和pH7.4条件下,自组装5,5.5层与10,10.5层相比较,蛋白的释放量差异无统计学意义(t=0.651～1.324,P>0.05)。加入OREC后,BSA释放量明显增加(t=2.264～2.305,P<0.05)。结论 BSA的释放受到环境的pH值、自组装层数、释放时间,以及是否加入OREC等因素的综合影响。
Abstract:
Objective To investigate the sustained releaseof BSA from chitosan-OREC/BSA films coated mats in vitro.MethodsThe negatively charged cellulose acetate(CA)fibrous mats were modified with multilayers of the positively charged chitosan or chitosan-OREC intercalated composites and the negatively charged bovine serum albumin(BSA)via electrostatic layer-by-layer(LBL)self-assembly technique.The adsorption and rinsing steps were repeated until the desired number of deposition bilayers was obtained.The in vitro BSA encapsulation and release experiments demonstrated thatOREC could affect the degree of protein loading capacity and release ficiency of the LBL films coating.Results In the pH-gradient release assay,only a small amount of BSA was released from the mats in 1 h.As thetime increased,the release rate of BSA of all the samples gradually went up tothe maximum data within 8 h.For the samples with identical number of bilayers and record time,obvious increasing of the release amount could be seen in pH 7.4,in comparison with pH 1.2.Besides,doubling bilayers film-coated mats generally.Meanwhile,it was slightly distinguishable between 5 and 5.5 as well as10 and 10.5 bilayers(t=0.651～1.324,P>0.05).Interestingly,it could beseen that protein release of the chitosan-OREC/BSA films coated mats remarkably increased compared with that of chitosan/BSA films coated mats(t=2.264～2.305,P<0.05).ConclusionThe release of protein in the initial time could be controlled by adjusting the number of deposition bilayers,the outmost layer and the composition of coating bilayers.

参考文献/References:

[1] Xu Y,Cui WG,Zhang YX,et al.Hierarchical micro/nanofibrous bioscaffolds for structural tissue regeneration[J].Adv Healthc Mater,2017,1601457.
[2] Sebe I,Ostorházi E,Bodai Z,et al.In vitro and in silico characterization of fibrous scaffolds comprising alternate colistin sulfate-loaded and heat-treated polyvinyl alcohol nanofibrous sheets[J].Int J Pharm,2017,523(1):151-158.
[3] Bian J,Olesik SV.Surface-assisted laser desorption/ionization time-of-flight mass spectrometry of small drug molecules and high molecular weight synthetic/biological polymers using electrospun composite nanofibers[J].Analyst,2017,142(7):1125-1132.
[4] Wu Y,Lin W,Hao H,et al.Nanofibrous scaffold from electrospinning biodegradable waterborne polyurethane/poly(vinyl alcohol)for tissue engineering application[J].J Biomater Sci Polym Ed,2017,28(7):648-663.
[5] Deng Y,Yang Y,Wei S.Peptide-decorated nanofibr-ous niche augments invitro directed osteogenic conversion of human pluripotent stem cells[J].Biomacromolecules,2017,18(2):587-598.
[6] 李红霞,国汉邦,周伟燕,等.血清高、低密度脂蛋白胆固醇标准物质的研究[J].现代检验医学杂志,2011,26(4):10-13. Li HX,Guo HB,Zhou WY,et al.Preparation of serum reference materials for high- and low- density lipoprotein cholesterol[J].J Mod Lab Med,2011,26(4):10-13.
[7] Chang SH,Lin HT,Wu GJ,et al.pH effects on solubility,zeta potential,and correlation between antibacterial activity and molecular weight of chitosan[J].Carbohydrate Polymers,2015,134:74-81.
[8] Ma J,Xin C,Tan CJ.Preparation,physicochemical and pharmaceutical characterization of chitosan from Catharsius molossus residue[J].International Journal of Biological Macromolecule,2015,80:547-556.
[9] Pradhan C,Dash S,Swain SK,et al.Barrier properties of nano silicon carbide designed chitosan nanocomposites[J].Carbohydrate Polymers,2015,134:60-65.
[10] Hassani S,Laouini A,Fessi H,et al.Preparation of chitosan-TPP nanoparticles using microengineered membranes-effect of parameters and encapsulation of tacrine[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2015,482(5):34-43.
[11] Jiang P,Li D,Xiao Y,et al.Preparation and characterization of chitosan-based core-shell microcapsules containing clove oil[J].J Nanosci Nanotechnol,2015,15(1):600-605.
[12] Dash M,Chiellini F,Ottenbrite RM,et al.Chitosan-A versatile semi-synthetic polymer in biomedical applications[J].Progress in Polymer Science,2011,36(8):981-1014.

备注/Memo

备注/Memo:
作者简介:徐瑞芬(1975-),女,博士,副主任医师,研究方向:纳米技术的生物医学应用,E-mail: 858215318@qq.com。 通讯作者:冯旭阳(1973-),男,博士,副教授,硕士研究生导师,研究方向:纳米技术的生物医学应用,E-mail:fengxuyang001@sohu.com。
更新日期/Last Update: 1900-01-01