[1]尹栩芳,樊一笋,汪 琪,等.人血清去唾液酸糖蛋白受体ASGPRH2亚基sH2a酶联免疫检测法的建立及临床应用[J].现代检验医学杂志,2017,32(05):8-12.[doi:10.3969/j.issn.1671-7414.2017.05.003]
　YIN Xu-fang,FAN Yi-sun,WANG Qi,et al.Establishment and Clinical Evaluation of sH2a Subunit from ASGPR in Diagnosis of Hepatic Injury[J].Journal of Modern Laboratory Medicine,2017,32(05):8-12.[doi:10.3969/j.issn.1671-7414.2017.05.003]
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人血清去唾液酸糖蛋白受体ASGPRH2亚基sH2a酶联免疫检测法的建立及临床应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
8-12
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Establishment and Clinical Evaluation of sH2a Subunit from ASGPR in Diagnosis of Hepatic Injury
文章编号:
1671-7414(2017)05-008-05
作者:
尹栩芳1; 樊一笋1; 汪 琪2; 饶品彬3; 石立立3
1.上海交通大学医学院附属苏州九龙医院检验科, 江苏苏州 215000; 2.江苏为真生物医药技术股份有限公司,江苏苏州 215000; 3.苏州吉玛基因股份有限公司,江苏苏州 215123
Author(s):
YIN Xu-fang1; FAN Yi-sun1; WANG Qi2; RAO Pin-bin3; SHI Li-li3
1.Department of Clinical Laboratory,Suzhou Kowloon Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Jiangsu Suzhou 215000,China; 2.Suzhou MicroDiag Biomedicine Co.Ltd,Jiangsu Suzhou 215000,China; 3.Suzhou Gene Pharma Co.Ltd,Jiangsu Suzhou 215123,China
关键词:
肝损伤;  去唾液酸糖蛋白受体;  sH2a;  酶联免疫检测
分类号:
R575; R446.61
DOI:
10.3969/j.issn.1671-7414.2017.05.003
文献标志码:
A
摘要:
目的 探讨肝损伤患者血清中去唾液酸糖蛋白受体(asialoglycoprotein receptor,ASGPR)的H2亚基(sH2a)水平和肝损伤进程的相关性。方法 纳入苏州九龙医院210例血清样本,其中70例肝损伤组患者,包括肝硬化、病毒性肝炎、脂肪肝,同时纳入140例对照组样本,包括高脂、溶血、黄疸、自身免疫性疾病、健康人。检测上述人群血清sH2a蛋白水平,对ELISA结果和临床诊断结果进行统计学分析。结果 ①140例对照组和70例肝损伤组的血清样本sH2a蛋白水平分别为105.92±53.41 ng/ml和69.25±27.45 ng/ml,差异有统计学显著性意义(F=14.375,t=5.397,P=0.000)。②sH2a蛋白水平诊断肝损伤的敏感度为68.57%(95%CI:56.37%～79.15%),特异度为82.86%(95%CI:75.58%～88.70%),总符合率为78.10%(95%CI:71.88%～83.49%),KAPPA系数:0.510 6(95%CI:0.387 7～0.633 6)。结论 血清sH2a ELISA检测试剂盒的符合率达到临床预期要求,可以作为新的血清标志物达到辅助诊断肝损伤相关疾病的效果。
Abstract:
Abstract:Objective To diagnose the process of hepatic injury,a method of quantitating the concentration of sH2a subunit from asialoglycoprotein receptor(ASGPR)in serum by enzyme-linked immunosorbent assay was established and clinical evaluated.Methods 210 serum samples were collected in Suzhou Kowloon Hospital.Among them,70 subjects with cirrhosis,viral hepatitis and fatty liver disease were as hepatic injury group and 140 subjects of healthy and high fat,hemolysis,jaundice and with autoimmune disease were as control group.The serum sH2a of two group were measured by ELISA kit.The results of sH2a ELISA were analyzed by four table chi-square test and SPSS20.0 software.Results sH2a protein level in liver injury group and control group were 105.92+53.41 ng/ml and 69.25+27.45 ng/ml,respectively.The difference between the control group and the liver injury group was statistically significant(F=14.375,t=5.397,P=0.000).The sensitivity andspecificity of the sH2a ELISA kit were 68.57%(95%CI:56.37%～79.15%)and 82.86%(95%CI:75.58%～88.70%),and the total compliance rate was 78.10%(95%CI:71.88%～83.49%)with KAPPA coefficient:0.510 6(95%CI:0.3877～0.6336).Conclusion SH2a serum ELISA kit with positive and negative coincidence rate between SH2a serum level and meet the clinical requirement,which could be used as new marker for diagnosis of heptical injury related diseases.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:尹栩芳(1974-),女,本科,副主任技师,主要从事临床生物化学、免疫学检验研究,E-mail:yxfd1225@sina.com。
更新日期/Last Update: 1900-01-01