[1]张宝华a,武 琪b.Nrf2基因启动子rs35652124,rs6706649和rs6721961位点单核苷酸多态性与阿尔茨海默病的相关性研究[J].现代检验医学杂志,2017,32(05):62-65.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　ZHANG Bao-huaa,WU Qib.Association between Nrf2 Gene rs35652124,rs6706649 and rs6721961 Polymorphism and Alzheimer's Disease[J].Journal of Modern Laboratory Medicine,2017,32(05):62-65.[doi:10.3969/j.issn.1671-7414.2017.05.017]
点击复制

Nrf2基因启动子rs35652124,rs6706649和rs6721961位点单核苷酸多态性与阿尔茨海默病的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
62-65
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Association between Nrf2 Gene rs35652124,rs6706649 and rs6721961 Polymorphism and Alzheimer's Disease
文章编号:
1671-7414(2017)05-062-04
作者:
张宝华a; 武 琪b
安康市中医医院 a.质控科; b.急诊科,陕西安康 725000
Author(s):
ZHANG Bao-huaa; WU Qib
a.Department of Medicine Quality Control; b.Department of Emergency,Ankang Traditional Chinese Hospital,Shaanxi Ankang 725000,China
关键词:
阿尔茨海默病;  核因子E2相关因子2;  基因多态性
分类号:
RR749.1; Q786
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨陕西地区汉族人群中,核因子E2相关因子2(Nrf2)基因多态性与阿尔茨海默病(AD)的相关性。方法 收集2015年1月～2016年12月于安康市中医医院治疗的382例AD患者,按照患者发病年龄分为早发性阿尔茨海默病(EOAD)患者116例和晚发性阿尔茨海默病(LOAD)患者266例,另选择120例认知功能正常志愿者作为对照组,收集所有入组成员的临床资料,并采用聚合酶链反应(PCR)联合DNA直接测序法检测Nrf2基因启动子rs35652124,rs6706649和rs6721961位点单核苷酸多态性。结果 EOAD组与对照组比较,rs35652124和rs6721961位点基因型和等位基因分布频率差异均有统计学意义(均P<0.05)。LOAD组与对照组比较,三个SNP位点基因型和等位基因分布频率差异均无统计学意义(均P>0.05)。对于EOAD组,rs35652124和rs6721961位点不同基因型患者,发病年龄差异具有统计学意义(P<0.05)。对于LOAD组,三个SNP位点不同基因型患者发病年龄差异无统计学意义(均P>0.05)。结论 Nrf2基因多态性可能与EOAD遗传易感性相关,且影响EOAD患者发病年龄。
Abstract:
Abstract:Objective To investigate the genetic association between NF-E2-related factor 2(Nrf2)gene polymorphism and Alzheimer's disease(AD)in the Han descent population of Shaanxi province.Methods382 AD patients from January 2015 to December 2016 were recruited to participate in the study.According to age of onset,they were divided into the early-onset Alzheimer's disease(EOAD)group(116 cases)and the late-onsetAlzheimer's disease(LOAD)group(266 cases).120 healthy volunteers without AD were recruited to participate in the study.Genotype was determined by polymerase chain reaction combined with DNA direct sequencing technique for the polymorphism of the Nrf2 gene.Results The genotype and allele distribution of rs35652124 and rs6721961 polymorphism were significantly different between the EOAD group and control group(P<0.05).There was no significant difference of genotype and allele distribution of three Nrf2 gene single nucleotide polymorphisms(SNPs)between the LOAD group and control group(P>0.05).For EOAD patients,age of onset was significantly different between different genotype of rs35652124 andrs6721961(P<0.05).However,there was no significant difference of age of onset in LOAD group(P>0.05).Conclusion Polymorphism of the Nrf2 gene may associate with the risk of AD,and affect disease progression.

参考文献/References:

[1] 徐向红,郭天康,苏海翔,等.中国汉族人群MAPT基因SNPs等位基因及单体型频率与阿尔茨海默病相关性研究[J].现代检验医学杂志,2014,29(2):34-37. Xu XH,Guo TK,Su HX,et al.Correlation study on SNPs of allele andhaplotype frequencies of MAPT gene with Alzheimers disease in han population ofChina[J].Journal of Modern Laboratory Medicine,2014,29(2):34-37.
[2] Pereira AC,Gray JD,Kogan JF,et al.Age and Alzheimer's disease gene expression profiles reversed by the glutamate modulator riluzole[J].Mol Psychiatry,2017,22(2):296-305.
[3] Greenough MA,Camakaris J,Bush AI.Metal dyshomeostasis and oxidative stress in Alzheimer's disease[J].Neurochem Int,2013,62(5):540-555.
[4] Zhang R,Zhang Q,Niu J,et al.Screening of microRNAs associated with Alzheimer's disease using oxidative stress cell model and different strains of senescence accelerated mice[J].J Neurol Sci,2014,338(1/2):57-64.
[5] Joshi G,Gan KA,Johnson DA,et al.Increased Alzheimer's disease-likepathology in the APP/PS1ΔE9 mouse model lacking Nrf2 through modulation of autophagy[J].Neurobiol Aging,2015,36(2):664-679.
[6] Sandberg M,Patil J,D'Angelo B,et al.NRF2-regulation in brain healthand disease:implication of cerebral inflammation[J].Neuropharmacology,2014,79(4):298-306.
[7] Cooper J.Diagnostic and statistical manual of mental disorders(4th Edition):DSM-IV[J].Br J Psychiatry,2001,179(1):97-98.
[8] 郝建华,王田园,张然蓉,等.阿尔茨海默病患者AD7C-NTP和Aβ42检测的价值探讨[J].现代检验医学杂志,2014,29(1):44-47. Hao JH,Wang TY,Zhang RR,et al.Significance of detecting urine AD7C-NTP and blood Aβ42 protein level of Alzheimer patients[J].Journal of Modern Laboratory Medicine,2014,29(1):44-47.
[9] Chakrabarti S,Sinha M,Thakurta IG,et al.Oxidative stress and amyloidbeta toxicity in Alzheimer's disease:intervention in a complex relationship byantioxidants[J].Curr Med Chem,2013,20(37):4648-4664.
[10] Bonet-Costa V,Pomatto LC,Davies KJ.The proteasome and oxidative stress in Alzheimer's disease[J].Antioxid Redox Signal,2016,25(16):886-901.
[11] Soodi M,Saeidnia S,Sharifzadeh M,et al.Satureja bachtiarica ameliorate beta-amyloid induced memory impairment,oxidative stress and cholinergic deficit in animal model of Alzheimer's disease[J].Metab Brain Dis,2016,31(2):395-404.
[12] Ruiz S,Pergola PE,Zager RA,et al.Targeting the transcription factorNrf2 to ameliorate oxidative stress and inflammation in chronic kidney disease[J].Kidney Int,2013,83(6):1029-1041.
[13] Terazawa R,Akimoto N,Kato T,et al.A kavalactone derivative inhibitslipopolysaccharide-stimulated iNOS induction and NO production through activation of Nrf2 signaling in BV2 microglial cells[J].Pharmacol Res,2013,71(5):34-43.
[14] Ashabi G,Alamdary SZ,Ramin M,et al.Reduction of hippocampal apoptosis by intracerebroventricular administration of extracellular signal-regulated protein kinase and/or p38 inhibitors in amyloid β rat model of Alzheimer's disease:involvement of nuclear related factor and nuclear factor[J].Basic Clin Pharmacol Toxicol,2013,112(3):145-155.
[15] Von Otter M,Landgren S,Nilsson S,et al.Nrf2-encoding NFE2L2,haplotypes influence disease progression but not risk in Alzheimer's disease and age-related cataract[J].Mech Ageing Dev,2010,131(2):105-110.

相似文献/References:

[1]武 琪a,张宝华b.Nrf2基因启动子-653G/A,-651G/A和-617C/A位点单核苷酸多态性与帕金森病易感性的关联性研究[J].现代检验医学杂志,2017,32(06):60.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　WU Qia,ZHANG Bao-huab.Association between Nrf2 Gene -653G/A,-651G/A and -617C/A Polymorphism and Susceptibility of Parkinson's Disease[J].Journal of Modern Laboratory Medicine,2017,32(05):60.[doi:10.3969/j.issn.1671-7414.2017.06.001]
[2]孙祝平,陈红英,陈思路.老年阿尔茨海默病患者血清Lp-PLA2，NLRP3 水平表达及其与认知功能损害的相关性[J].现代检验医学杂志,2020,35(01):49.[doi:10.3969/j.issn.1671-7414.2020.01.013]
　SUN Zhu-ping,CHEN Hong-ying,CHEN Si-lu.Expression of Serum Lp-PLA2 and NLRP3 in Elderly Patients with Alzheimer's Disease and Its Relationship with Cognitive Impairment[J].Journal of Modern Laboratory Medicine,2020,35(05):49.[doi:10.3969/j.issn.1671-7414.2020.01.013]
[3]李婷婷,陈 凤,刘 然.阿尔茨海默病患者血清FGF23 和ZAG 水平表达与认知功能的相关性分析[J].现代检验医学杂志,2023,38(06):166.[doi:10.3969/j.issn.1671-7414.2023.06.030]
　LI Tingting,CHEN Feng,LIU Ran.Correlation Analysis between Serum FGF23 and ZAG Levels and Cognitive Function in Patients with Alzheimer’s Disease[J].Journal of Modern Laboratory Medicine,2023,38(05):166.[doi:10.3969/j.issn.1671-7414.2023.06.030]
[4]刘 倩,边 娜,孙旸园.阿尔茨海默病患者脑脊液SMOC1和OLFML3水平表达及对病情程度的评估价值[J].现代检验医学杂志,2024,39(06):124.[doi:10.3969/j.issn.1671-7414.2024.06.021]
　LIU Qian,BIAN Na,SUN Yangyuan.Expression of SMOC1 and OLFML3 in Cerebrospinal Fluid of Patients with Alzheimer’s Disease and Its Value in Evaluating the Severity of the Disease[J].Journal of Modern Laboratory Medicine,2024,39(05):124.[doi:10.3969/j.issn.1671-7414.2024.06.021]

备注/Memo

备注/Memo:
作者简介:张宝华(1977-),男,本科,主治医师,研究方向:神经内科常见病诊治,E-mail:zhangbh1977bh@163.com。 通讯作者:武 琪,E-mail:wuqi197510@126.com。
更新日期/Last Update: 1900-01-01