[1]王 萍a,张 熊b.血清β2-AR mRNA和NGF mRNA水平检测在儿童支气管哮喘气道重塑中的应用价值[J].现代检验医学杂志,2017,32(05):69-73.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　WANG Pinga,ZHANG Xiongb.Clinic Application of Levels of Serum β2-AR mRNA and NGF mRNA for Children with the Bronchial Asthma in Airway Remodeling[J].Journal of Modern Laboratory Medicine,2017,32(05):69-73.[doi:10.3969/j.issn.1671-7414.2017.05.017]
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血清β2-AR mRNA和NGF mRNA水平检测在儿童支气管哮喘气道重塑中的应用价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
69-73
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Clinic Application of Levels of Serum β2-AR mRNA and NGF mRNA for Children with the Bronchial Asthma in Airway Remodeling
文章编号:
1671-7414(2017)05-069-05
作者:
王 萍a; 张 熊b
咸阳市第一人民医院a.儿科; b.检验科,陕西咸阳 712000
Author(s):
WANG Pinga; ZHANG Xiongb
a.Department of Pediatrics; b.Department of Clinical Laboratory, the First People's Hospital of Xianyang City,Shaanxi Xianyang 712000,China
关键词:
儿童支气管哮喘;  气道重塑;  治疗;  miRNA-155;  miRNA-26a
分类号:
R725.6; Q503
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨血清β2-肾上腺素受体(β2-AR)mRNA和组织神经生长因子(NGF)mRNA水平的检测在儿童支气管哮喘气道重塑中的应用价值。方法 收集2015年3月～2017年3月在咸阳市第一人民医院儿科门诊就诊或住院的33例发生气道重塑的支气管哮喘患儿的血液及临床资料,并选取29例呼吸系统炎症疾病患儿作为对照组,采用实时荧光定量PCR(Real-time-PCR,RT-PCR)技术检测患儿治疗前后血清β2-AR mRNA和NGF mRNA的表达。比较分析血清β2-AR mRNA和NGF mRNA的变化与哮喘重塑发生之间的关系。采用高分辨螺旋CT,测量受试者气道的内径(L)、外径(D),通过公式T=(D-L)/2计算气道壁的厚度(T),测量气道腔面积(AI)、气道总横截面积(AO),通过以上指标评价哮喘气道重塑的发生。采用受试者工作特征曲线(ROC曲线)评价血清β2-AR mRNA和NGF mRNA的临床意义。结果 与对照组比较,治疗后组的血清β2-AR mRNA水平下调和NGF mRNA水平上调,其差异均有统计学意义(t=37.27,36.53,均P<0.01)。与治疗前组比较,治疗后组的血清β2-AR mRNA和NGF mRNA水平下调,其差异均有统计学意义(t=7.95,12.67,均P<0.01)。治疗后组血清β2-AR mRNA和NGF mRNA水平呈现负相关性(r=-0.697,P<0.01),该组两标志物分别与气道内径(L),气道壁的厚度(T)及气道腔面积(AI)有明显相关性(r=0.775,-0.780,0.793,均P<0.01),(r=-0.750,0.763,-0.779,均P<0.01)。治疗前组血清β2-AR mRNA和NGF mRNA水平呈现负相关性(r=-0.703,P<0.01),该组两标志物分别与气道内径(L),气道壁的厚度(T)及气道腔面积(AI)有明显相关性(r=0.782,-0.797,0.803,均P<0.01),(r=-0.772,0.787,-0.809,均P<0.01)。血清β2-AR mRNA和NGF mRNA在治疗后表达量的AUC分别为0.767(95%CI:0.658～0.876,P=0.000); 0.744(95%CI:0.632～0.856,P=0.000)。根据SPSS统计结果计算出β2-AR和NGF在最佳临界值处诊断重塑的敏感度和特异度分别为93.9%和96.2%,92.9%和95.7%。结论 检测血清β2-AR mRNA和NGF mRNA水平可应用于儿童支气管哮喘气道重塑疗效的评价,估计病情的发展状况,并为哮喘患儿的早期干预治疗、有效控制气道炎症及逆转哮喘病程进展提供依据。
Abstract:
Abstract:Objective To investigate the clinical application value of detection of serum beta 2 adrenergic receptor(beta 2-AR)mRNA and nerve tissue growth factor(NGF)mRNA in airway remodeling on children with bronchial asthma.Methods The clinical serum data of 33 casesof children with bronchial asthma in airway remodeling were selected,divided into the pre-treatment group and the post-treatment group,and selected 29 cases of children with respiratory diseases as the control group.The above research subjects were outpatients or inpatients from department of pediatrics in theFirst People's Hospital of Xianyang City from 2015.3 to 2017.3.The expression of serum beta 2-ARmRNA and NGFmRNA were detected by real-time fluorescencequantitative PCR(Real-time-PCR,RT-PCR).The relationship between the changes of serum 2-ARmRNA and NGFmRNA and the occurrence of asthma remodeling was compared and analyzed.All the subjects were measured the inner diameter of the airway(L),diameter of the airway(D),the airway wall thickness(T)calculated by T=(D-L)/2,the airway lumen area(AI),total airway cross-sectional area(AO)with High Resolution Computerized Tomography in evaluation of airway remodelingof asthma through the above indexes.The clinical significance of serum beta 2-ARmRNA and NGFmRNA by receiver operating characteristic curve(ROC curve)wasevaluated.Results The serum beta 2-ARmRNA and NGFmRNA expression in the post-treatment group were,respectively,up-regulation and down-regulation in comparison with the pre-treatment group,and there was significant difference(t=37.27,36.53,all P<0.01),which in the post-treatment group were,respectively,down-regulation and up -regulation in comparison with the control group,and there was significant difference(t=7.95,12.67,all P<0.01).The expression of erum beta 2-ARmRNA and NGFmRNA had anegative correlation in the remodeling group(r=-0.697,P<0.01),which was associated obviously respectively with L,T and AI(r=0.775,-0.780,0.793,all P<0.01),(r=-0.750,0.763,-0.779,all P<0.01).The expression of erum beta 2-ARmRNA and NGFmRNA had a negative correlation in the pre-treatment group(r=-0.703,P<0.01),which was associated obviously respectively with L,T and AI(r=0.782,-0.797,0.803,all P<0.01),(r=-0.772,0.787,-0.809,all P<0.01).According to the statistical results of SPSS,ROC curve analysis showed that the expression levels of serum beta 2-ARmRNA and NGFmRNA in the remodeling group were 0.767(95%CI:0.658～0.876,P=0.000); 0.744(95%CI:0.632～0.856,P=0.000).The sensitivity and specificity of beta 2-ARmRNA and NGF mRNA at the optimal critical valuewere 93.9% and 96.2%,92.9% and 95.7%.Conclusion The expression of serum beta 2-ARmRNA and NGFmRNA can monitor treatment of airwayremodeling on children bronchial asthma,and estimate disease development,effectively control the progress of airway inflammation in reversing asthma diseaseprogression to provide the basis for early intervention of children with bronchial asthma.

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备注/Memo

备注/Memo:
作者简介:王 萍(1976-),女,本科学历,主治医师,研究方向:哮喘的诊断与治疗,E-mail:1271939973@qq.com。 通讯作者:张 熊(1968-),男,本科学历,副主任检验师,研究方向:疾病的基因诊断,E-mail:418079040@qq.com。
更新日期/Last Update: 1900-01-01