[1]巨 涛,宋 波,刘文刚,等.血清PPAR-γ mRNA,MMP-9 mRNA检测与颅内动脉瘤破裂相关的临床应用研究[J].现代检验医学杂志,2017,32(05):83-86.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　JU Tao,SONG Bo,LIU Wen-gang,et al.Clinical Application of Detecting Serum PPAR-γ mRNA, MMP-9 mRNA in the Diagnosis of Ruptured Intracranial Aneurysm[J].Journal of Modern Laboratory Medicine,2017,32(05):83-86.[doi:10.3969/j.issn.1671-7414.2017.05.017]
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血清PPAR-γ mRNA,MMP-9 mRNA检测与颅内动脉瘤破裂相关的临床应用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
83-86
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Clinical Application of Detecting Serum PPAR-γ mRNA, MMP-9 mRNA in the Diagnosis of Ruptured Intracranial Aneurysm
文章编号:
1671-7414(2017)05-083-04
作者:
巨 涛; 宋 波; 刘文刚; 岳新鹏; 李 鲁; 刘增强
延安大学咸阳医院神经外科,陕西咸阳 712000
Author(s):
JU Tao; SONG Bo; LIU Wen-gang; YUE Xin-peng; LI Lu; LIU Zeng-qiang
Departerment of Neurosurgery,the Xianyang Hospital of Yan'an University,Shaanxi Xianyang 712000,China
关键词:
颅内动脉瘤;  颅内动脉瘤破裂;  过氧化物酶体增殖物激活受体-γ mRNA;  基质金属蛋白酶-9 mRNA
分类号:
R743.9; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨血清过氧化物酶体增殖物激活受体γ(PPAR-γ)mRNA、基质金属蛋白酶9(MMP-9)mRNA检测在诊断颅内动脉瘤破裂中的临床应用价值。方法 通过RT-PCR技术检测87例颅内动脉瘤患者血清PPAR-γ mRNA,MMP-9 mRNA的表达,其中破裂组57例,未破裂组30例,并设立对照组,比较以上指标的变化。结果 破裂组、未破裂组和对照组血清PPAR-γ mRNA的表达量分别为0.23±0.03,0.59±0.11和0.87±0.15; 血清MMP-9 mRNA在三组中的表达量分别为0.93±0.17,0.63±0.13和0.25±0.05。破裂组血清PPAR-γ mRNA表达量与对照组比较明显下调(t=23.79,P<0.01),而血清MMP-9 mRNA表达量则明显上调,差异有统计学意义(t=25.63,P<0.01); 血清PPAR-γ mRNA的表达在破裂组的表达量低于未破裂组,而MMP-9 mRNA表达量高于未破裂组,差异有统计学意义(t=15.32,16.27,P<0.01)。建立受试者工作特征曲线(ROC曲线)评价两标志物的临床意义,以破裂组和未破裂组为因变量,血清PPAR-γ mRNA,MMP-9 mRNA表达量的AUC分别为0.858(95%CI:0.775～0.940,P=0.000); 0.842(95%CI:0.756～0.929,P=0.000)。以对照组和未破裂组为因变量,血清PPAR-γ mRNA,MMP-9 mRNA表达量的AUC分别为0.827(95%CI:0.734～0.920,P=0.000); 0.818(95%CI:0.722～0.914,P=0.000)。结论 血清PPAR-γ mRNA,MMP-9 mRNA的检测可应用于颅内动脉瘤发生及病程进展的评价,并为颅内动脉瘤破裂的预测提供依据。
Abstract:
Abstract:Objective To investigate the clinical application of detecting serum PPAR-γmRNA,MMP-9mRNA in the diagnosis of ruptured intracranial aneurysm.Methods The expression of serum PPAR-γmRNA,MMP-9mRNA were detected for 87 cases of patients with intracranial aneurysm,including ruptured group and non-ruptured group,respectively,with 57 cases and 30 cases of patients.The control group should be established to comparethe changes of the above indicators.Results The expression of serum PPAR-γmRNA in the ruptured group,the non-ruptured and the controls group were 0.23±0.03,0.59±0.11 and 0.87±0.15,which of MMP-9mRNAwere 0.93±0.17,0.63±0.13 and 0.25±0.05.Compared with those in the controls group,the expression of serum PPAR-γmRNA in the ruptured group significantly lowered(t=23.79,P<0.01),which of MMP-9mRNA raised(t=25.63,P<0.01).There were statistically significant differences.The expression ofPPAR-γmRNA in the ruptured group were lower than those in the unruptured group,which of MMP-9 mRNA were higher(t=15.32,16.27,P<0.01).To establishing the receiver-operating characteristic curve(ROC curve)in evaluating theclinical significances of the two markers to use the rupture group and non-ruptured group as the dependent variable,the AUC of the expression of serum PPAR-γmRNA,MMP-9mRNA were 0.858(95%CI:0.775～0.940,P=0.000),0.842(95%CI:0.756～0.929,P=0.000).As the dependent variable in the control group and unruptured group,the AUC of the expression of serum PPAR-γmRNA,MMP-9mRNA were 0.827(95%CI:0.734～0.920,P=0.000); 0.818(95%CI:0.722～0.914,P=0.000).Conclusion Detection of serum PPAR-γ mRNA,MMP-9 mRNA can be applied in assessment of occurrence and progression for the intracranial aneurysm,and to provide evidences for the early detection of ruptured intracranial aneurysm.

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备注/Memo

备注/Memo:
作者简介:巨 涛(1981-),男,硕士研究生,主治医师,研究方向:脑出血的诊断与治疗,E-mail:3248089878@qq.com。 通讯作者:刘增强(1976-),男,本科学历,副主任医师,研究方向:脑出血的诊断与治疗,E-mail:3395807407@qq.com。
更新日期/Last Update: 1900-01-01