[1]李 勇,潘秋亚,朱寿华.儿童支原体肺炎患者血清中NF-κB及TNF-α的表达及临床意义[J].现代检验医学杂志,2017,32(05):87-89,93.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　LI Yong,PAN Qiu-ya,ZHU Shou-hua.Expression and Its Significance of Serum NF-κB and TNF-α in Children with Mycoplasma Pneumoniae Pneumonia[J].Journal of Modern Laboratory Medicine,2017,32(05):87-89,93.[doi:10.3969/j.issn.1671-7414.2017.05.017]
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儿童支原体肺炎患者血清中NF-κB及TNF-α的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
87-89,93
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Expression and Its Significance of Serum NF-κB and TNF-α in Children with Mycoplasma Pneumoniae Pneumonia
文章编号:
1671-7414(2017)05-087-04
作者:
李 勇1; 潘秋亚2; 朱寿华3
1.高邮市中医医院检验科,江苏高邮 225600; 2.常州市儿童医院检验科,江苏常州 213000; 3.宝应县妇幼保健院,江苏宝应225800
Author(s):
LI Yong1; PAN Qiu-ya2; ZHU Shou-hua3
1.Department of Clinical Laboratory, Gaoyou Traditional Chinese Medicine Hospital,Jiangsu Gaoyou 225600,China; 2.Department of Clinical Laboratory,Changzhou Children's Hospital,Jiangsu Changzhou 213000, China; 3.Baoying Maternal and Children Health Care Hospital,Jiangsu Baoying225800,China
关键词:
支原体肺炎;  核转录因子-κB;  肿瘤坏死因子-α
分类号:
R725.6; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨核转录因子(NF-κB)及肿瘤坏死因子(TNF-α)在儿童肺炎支原体肺炎(MPP)中表达水平及其辅助诊断价值。方法 纳入高邮市中医医院、常州市儿童医院、宝应县妇幼保健院2016年01月～2017年01月收治的MPP患儿150例,根据症状将其分为喘息组(75例)及非喘息组(75例),同期匹配正常健康儿童(75例)作为对照组,收集不同组别儿童血液样本并提取血清后,通过实时荧光定量PCR检测比较三组人群血清中NF-κB及TNF-α的表达量差异。应用ROC曲线及AUC(95%CI)寻找其作为诊断指标的最佳临界值,同时评估其诊断效能。结果 MPP喘息组患儿血清中NF-κB及TNF-α的相对表达量分别为3.09±0.15和2.14±0.11,两者较正常对照组均升高,差异有统计学意义(t=11.03,6.987,均P<0.05)。MPP非喘息组患儿血清NF-κB及TNF-α的相对表达量分别为2.28±0.11和1.61±0.08,两者较正常对照组均升高,差异有统计学意义(t=6.873,7.406,均P<0.05)。喘息组患儿血清中NF-κB及TNF-α的相对表达量较非喘息组明显升高,差异有统计学意义(P均<0.05)。NF-κB诊断MPP的最佳临界值为1.895,其敏感度为71.33%,特异度为91.89%,AUC为0.8265,95%CI为0.772 8～0.880 2; TNF-α诊断MPP的最佳临界值为1.450,其敏感度为63.00%,特异度为90.54%,AUC为0.818 3,95%CI为0.765 1～0.871 6。结论 MPP患儿血清中NF-κB,TNF-α的表达量均较正常对照组升高,血清NF-κB及TNF-α可作为儿童MPP的辅助诊断指标。
Abstract:
Abstract:Objective To explore the diagnostic value of the quantitative detection of serum NF-κB and TNF-α in children with Mycoplasma pneumoniae Pneumonia(MPP).Methods 150 MPP patients were enrolled in this study including 75 with wheeze and 75 without wheeze,and then collected the blood specimens.75 healthy children were recruited to the control group(NC group).The levels of NF-κB and TNF-α in different groups were detected by qRT-PCR.The performance of NF-κB and TNF-α were evaluated by receiver operating characteristic curves(ROC)and the area under the curve(AUC)(95%CI).Results The relative expression of NF-κB and TNF-α in MPP children with wheeze were 3.09±0.15 and 2.14±0.11,respectively.They were significantly higher than NC group(t=11.03,6.987,P<0.05).NF-κB and TNF-α in MPP children without wheeze were also significantly higher than control group(t=6.873,7.406,P<0.05).The relative expression of NF-κB and TNF-α in children with wheeze were significantly higher than children without wheeze.The detection of NF-κB yielded an area under the curve of ROC of 0.826 5(95%CI:0.772 8～0.880 2)in discriminating MPP patients from healthy group.The TNF-α was 0.818 3(95% CI:0.765 1～0.871 6)in discriminating MPP patients from healthy group.Conclusion The relative expression of NF-κB and TNF-α in children with MPP was significantly higher than that in NC group,NF-κB and TNF-α may prove to be non-invasive biomarkers for the auxiliary diagnosis of MPP.

参考文献/References:

[1] Diaz MH,Benitez AJ,Winchell JM.Investigations of Mycoplasmapneumoniae infections in the United States:trends in molecular typing and macrolide resistance from 2006 to 2013[J].J Clin Microbiol,2015,53(1):124-130.
[2] 赵 岩,谢珊辉,卢丽萍.儿童肺炎支原体感染IgM抗体和DNA检测结果的应用分析[J].现代检验医学杂志,2015,30(3):80-82,86. Zhao Y,Xie SH,Lu LP.Application analysis of IgM antibody and DNAtest results in children Mycoplasma pneumonia infection[J].Journal of Modern Laboratory Medicine,2015,30(3):80-82,86.
[3] Ye Q,Shao WX,Shang SQ,et al.Epidemiological characteristics and immune status of children with respiratory syncytial virus[J].J Med Virol,2015,87(2):323-329.
[4] 中华医学会儿科学分会呼吸学组,《中华实用儿科临床杂志》编辑委员会.儿童肺炎支原体肺炎诊治专家共识(2015年版)[J].中华实用儿科临床杂志,2015,30(17):1304-1308. Respiratory Branch of Chinese Pediatric Society of Chinese Medical Association,Editorial Board of Chinese Journal of Applied Clinical Pediatrics.Expert consensus on diagnosis and treatment of Mycoplasma pneumoniae pneumonia in children(2015)[J].Chinese Journal of Applied Clinical Pediatrics,2015,30(17):1304-1308.
[5] Jain S,Williams DJ,Arnold SR,et al.Community-acquired pneumonia requiring hospitalization among U.S children[J].N Engl J Med,2015,372(9):835-845.
[6] Harris M,Clark J,Coote N,et al.British thoracic society guidelines for the management of community acquired pneumonia in children:update 2011[J].Thorax,2011,66(Suppl 2):ii1-ii23.
[7] 马志超,黄白丽,阮和球,等.降钙素原联合呼吸道病原体抗体检测在小儿肺炎的诊断价值[J].现代检验医学杂志,2015,30(2):144-145,148. Ma ZC,Huang BL,Ruan HQ,et al.Diagnosis value of PCT combined with respiratory pathogen detection in pneumonia in children[J].Journal of Modern Laboratory Medicine,2015,30(2):144-145,148.
[8] 徐祖龙,董苏荣,张亚明.WBC,CRP和ESR联合检测在肺炎支原体肺炎中的意义[J].现代检验医学杂志,2015,30(1):153-155,158. Xu ZL,Dong SR,Zhang YM.Significance of combining with WBC,CRP and ESR detection in patients with Mycoplasma pneumoniae pneumonia[J].Journal of Modern Laboratory Medicine,2015,30(1):153-155,158.
[9] Ye Q,Xu XJ,Shao WX,et al.Mycoplasma pneumoniae infection in children is a risk factor for developing allergic diseases[J].Scientific World Journal,2014,2014(6,articlez):986527.
[10] 任 勇,李 楠,苏红梅,等.支原体肺炎患儿TNF-α,IL-6及IL-10的变化及其临床意义探讨[J].当代医学,2015,21(2):89-90. Ren Y,Li N,Su HM,et al.Levels of TNF-α,IL-6 and IL-10 in childrenwith Mycoplasma pneumoniae pneumonia[J].Contemporary Medicine,2015,21(2):89-90.
[11] Goldhaber SZ,Bounameaux H.Pulmonary embolism and deep vein thrombosis[J].Lancet,2012,379(9828):1835-1846.
[12] 庞焕香,乔红梅,成焕吉,等.支原体肺炎患儿肺泡灌洗液中TNF-α,IL-6,IL-10水平检测及意义[J].中国当代儿科杂志,2011,13(10):808-810. Pang HX, Qiao HM, Cheng HJ,et al.Levels of TNF-α,IL-6 and IL-10 inbronchoalveolar lavage fluid in children with Mycoplasma pneumoniae pneumonia[J].Chinese Journal of Contemporary Pediatrics,2011,13(10):808-810.
[13] 董西华,周 霞,周立平,等.丙肝患者血清HCV RNA与TNF-α之间的关系及其与肝损伤程度相关性的研究[J].现代检验医学杂志,2011,26(6):21-23,27. Dong XH,Zhou X,Zhou LP,et al.Study of the relationship between HCV-RNA and TNF-α in serum and their relevance with degree of liver injury in HCV infectors[J].Journal of Modern Laboratory Medicine,2011,26(6):21-23,27.
[14] Bui TT,Piao CH,Kim SM,et al.Citrus tachibana leaves ethanol extractalleviates airway inflammation by the modulation of Th1/Th2 imbalance via inhibiting NF-κB signaling and histamine secretion in a mouse model of allergic asthma[J].J Med Food,2017,20(7):676-684.
[15] Dorronsoro A,Ferrin I,Salcedo JM,et al.Human mesenchymal stromal cells modulate T-cell responses through TNF-α-mediated activation of NF-κB[J].Eur J Immunol,2014,44(2):480-488.
[16] Gómez-SanMiguel AB,Villanúa MA,Martín AI,et al.D-TRP(8)-γ MSHprevents the effects of endotoxin in rat skeletal muscle cells through TNFα/NF-RB signalling pathway[J].PLoS One,2016,11(5):e0155645.

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备注/Memo

备注/Memo:
作者简介:李 勇(1972-),男,大学本科,学士,副主任技师,主要从事临床检验与研究,E-mail:59979133@qq.com。 通讯作者:朱寿华(1970-),男,大学本科,学士,副主任技师,主要从事临床检验与研究,E-mai:13651515363@139.com。
更新日期/Last Update: 1900-01-01