[1]于菲菲,庞白冰,杨 华,等.单纯性2型糖尿病与糖尿病肾病患者尿液中α1-微球蛋白和激肽原1的表达分析[J].现代检验医学杂志,2017,32(05):94-96，99.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　YU Fei-fei,PANG Bai-bing,YANG Hua,et al.Expression Analysis of α1-Microglobulin,Kininogen1 in Urine of Simple Type 2 Diabetes and Diabetic Nephropathy Patients[J].Journal of Modern Laboratory Medicine,2017,32(05):94-96，99.[doi:10.3969/j.issn.1671-7414.2017.05.017]
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单纯性2型糖尿病与糖尿病肾病患者尿液中α1-微球蛋白和激肽原1的表达分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
94-96，99
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Expression Analysis of α1-Microglobulin,Kininogen1 in Urine of Simple Type 2 Diabetes and Diabetic Nephropathy Patients
文章编号:
1671-7414(2017)05-094-04
作者:
于菲菲; 庞白冰; 杨 华; 廖国玲
宁夏医科大学临床医学院,银川 750004
Author(s):
YU Fei-fei; PANG Bai-bing; YANG Hua; LIAO Guo-ling
School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China
关键词:
糖尿病肾病;  α1-微球蛋白;  激肽原1
分类号:
R587; R446.112
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 研究尿α1-微球蛋白(α1-MG)和尿激肽原1(KNG1)水平在单纯性2型糖尿病与糖尿病肾病患者中的表达变化情况。方法 选取2016年9月～2017年3月在宁夏医科大学总医院住院的糖尿病肾病患者34例作为实验组(DN组)以及同时期单纯性2型糖尿病患者32例作为对照组(DM组),其中,DN组包括甲组(HbAlc<10%)19例和乙组(HbAlc≥10%)15例。测定尿α1-MG和尿KNG1及各项生化指标。结果 与DM组相比,尿α1-MG在DN组下降(t=9.972,P<0.01),尿KNG1在DN组升高(t=-3.356,P<0.01),差异均具有统计学意义; DN乙组尿α1-MG、血清GLU水平均高于DN甲组(t=-2.092,-3.464,均P<0.05),DN乙组血清Cr水平低于DN甲组,差异具有统计学意义(t=2.181,P<0.05); 此外,在DN组,根据Pearson相关分析显示,尿α1-MG与HbAlc,尿KNG1呈正相关(r=0.33和0.355,均P<0.05),其它未见明显相关。结论 尿α1-MG和KNG1的表达在DN的发生发展中存在一定的变化规律,可为DN的临床诊断提供依据。
Abstract:
Abstact:Objective To explore the changes of detection ofurine α1-MG and KNG1 in simple type 2 diabetes mellitus and diabetic nephropathy patients.Methods 66 cases of diabetic patients in General Hospital of Ningxia Medical University from September 2016 to March 2017 were recruited,and the patients were divided into two groups,as follows: the diabetic nephropathy(DN)group 34 cases as experimental group,and the simple type 2diabetes mellitus(DM)group 32 cases as control group.Than DN group was divided into two groups according to HbAlc,A groups 19 cases HbAlc was less than 10%,B groups 15 cases HbAlc was greater than or equal to 10%.The urine α1-MG,urine KNG1 and other biochemical indicators were mearured for all subjects.Results Compared with the DM group,the urine α1-MG in DN group was significantly lower(t=9.972,P<0.01),but the result of the urine KNG1 was the opposite(t=-3.356,P<0.01).The urine α1-MG and serum GLU in B group were significantly higher than those of the A group(t=-2.092,-3.464,all P<0.05),but the result of the serum Cr was the opposite(t=2.181,P<0.05).The level of urine α1-MG in DN group were positively correlated with HbAlc and urine KNG1(r=0.33,0.355,all P<0.05).Conclusion There were some changes in the expression of urine α1-MG and KNG1 in the occurrence and development of diabetic nephropathy,which provide the basis for the clinical diagnosis of diabetic nephropathy.

参考文献/References:

[1] Bloomgarden ZT.Diabetes:East meets West.the joint American Association of Clinical Endocrinologists-Chinese Society of Endocrinology(AACE-CSE)Symposium[J].Journal of Diabetes,2012,4(3):221-226.
[2] 黄 俊,王永红,马华兰.血清胱抑素C,β2微球蛋白、尿微量白蛋白联合检测在糖尿病肾病早期诊断中的临床价值[J].中国卫生检验杂志,2017,27(1):54-56. Huang J,Wang YH,Ma HL.The clinical value of combined detection ofserum cystatin C,β2-microglobulin and urinary albumin in the early diagnosisof diabetic nephropathy[J].Chinese Journal of Health Laboratory Technology,2017,27(1):54-56.
[3] Susztak K,B(¨overo)ttinger EP.Diabetic nephropathy:a fro-ntier forpersonalized medicine[J].Journal of the American Society of Nephrology:JASN,2006,17(2):361-367.
[4] 谭婷婷,程 莉,魏红霞,等.尿α1-酸性糖蛋白在早期糖尿病肾病诊断中的应用价值[J].现代检验医学杂志,2016,31(1):80-82. Tan TT,Cheng L,Wei HX,et al.Value of urinary α1-acid glycoprotein in early diagnosis of diabetic nephropathy[J].Journal of Modern Laboratory Medicine,2016,31(1):80-82.
[5] 向代军,张洪瑞,白 洁,等.尿α1微球蛋白和β2微球蛋白对2～4期慢性肾病患者的诊断价值[J].检验医学与临床,2014,11(8):1011-1012,1015. Xiang DJ,Zhang HR,Bai J,et al.Value of urine α1-microglobuminand β2-microglobumin for the diagnosis of stage 2～4 chronic kidney disease[J].Laboratory Medicine & Clinic,2014,11(8):1011-1012,1015.
[6] Vitova L,Tuma Z,Moravec J,et al.Early urinary biomarkers of diabeticnephropathy in type 1 diabetes mellitus show involvement of kallikrein-kinin system[J].BMC Nephrology,2017,18(1):112.
[7] 朱 祎,沈惠风.糖尿病肾病发病机制的研究进展[J].中国医师进修杂志,2012,35(10):68-70. Zhu Y,Shen HF.Update on the pathogenesis of diabetic nephropathy[J]. Chinese Journal of Postgraduates of Medicine,2012,35(10):68-70.
[8] Currie G,Mckay G,Delles C.Biomarkers in diabetic nephropathy:Present and future[J].World Journal of Diabetes,2014,5(6):763-776.
[9] 朱海兵.尿微量白蛋白、胱抑素C、β2微球蛋白和尿微量白蛋白/肌酐比值检测在2型糖尿病早期肾损害诊断的临床意义[J].实验与检验医学,2016,34(1):84-86. Zhu HB.The clinical significance of urine microalbumin,cystatin C,β2-microglobulin and urine microalbumin /creatinine ratio detection early renal damage in type 2 diabetes diagnosis[J].Experimental and Laboratory Medicine,2016,34(1):84-86.
[10] 赵建军,朱 武.血清胱抑素C,同型半胱氨酸及尿微量清蛋白/肌酐比值在糖尿病肾病早期诊断中的应用[J].现代检验医学杂志,2014,29(2):117-120. Zhao JJ,Zhu W.Application of serum cystatin C,homocysteine,the ratio between microalbuminuria and creatinine in the early diagnosis of the diabetic nephrosis[J].Journal of Modern Laboratory Medicine,2014,29(2):117-120.
[11] 姚碧婉.多项生化检测指标对糖尿病肾病的早期诊断价值[J].国际检验医学杂志,2014,35(14):1873-1874,1877. Yao BW.Values of multiple biochemical indexes in diagnosis of early diabetic nephropathy[J].International Journal of Laboratory Medicine,2014,35(14):1873-1874,1877.
[12] Akerstr MB,L(¨overo)gdberg L,Berggard T,et al.Alpha(1)-Microglobulin:a yellow-brown lipocalin[J].Biochimica Et Biophysica Acta,2000,1482(1/2):172-184.
[13] Grubb A.Diagnostic value of analysis of cystatin C and protein HC in biological fluids[J].Clinical Nephrology,1992,38(Suppl 1):S20-27.
[14] 魏雁虹,杨广民.α1微球蛋白的生物学特性及临床应用研究进展[J].国际检验医学杂志,2012,33(17):2111-2113. Wei YH,Yang GM.α1-Microglobulin biological cha-racteristics and clinical application research progress[J].International Journal of LaboratoryMedicine,2012,33(17):2111-2113.
[15] Pompach P,Ashline DJ,Brnakova Z,et al.Protein and site specificity of fucosylation in liver-secreted glycoproteins[J].Journal of Proteome Research,2014,13(12):5561-5569.
[16] Sharma JN,Narayanan P.The kallikrein-kinin pathways in hypertension and diabetes[J].Prog Drug Res,2014(69):15-36.
[17] Gonzalez Calero L,Martin-Lorenzo M,Ramos-Barron A,et al.Urinary kininogen-1 and retinol binding protein-4 respond to acute kidney injury:predictors of patient prognosis[J].Sci Rep,2016(6):19667.
[18] Katorl M,Majima M.A missing link between a high salt intake and bloodpressure increase[J].Journal of Pharmacological Sciences,2006,100(5):370-390.

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备注/Memo

备注/Memo:
基金项目:宁夏医科大学临床医学一流学科建设项目(First-class discipline construction of clinical medicine of Ningxia Medica university); 2017年宁夏“研究生教育创新计划”学位点建设项目[The plan of postgraduate Education Innovation,Discipline Construction project of Ningxia,China(2017)(YXW 2017014)]; 宁夏高等学校科学研究重点项目(NGY2013058)。 作者简介:于菲菲(1990-),女,硕士研究生,研究方向:临床检验诊断学,E-mail:m15769591302@163.com。 通讯作者:廖国玲(1967-),女,教授,硕士研究生导师,E-mail:guolingliao@163.com。
更新日期/Last Update: 1900-01-01