[1]喻 晶,王 琳,卢丽华,等.淋巴细胞及血浆EB病毒DNA在EBV感染相关疾病中表达的研究[J].现代检验医学杂志,2017,32(06):15-18.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　YU Jing,WANG Lin,LU Li-hua,et al.Expression of Epstein-Barr Virus DNA in Peripheral Blood Lymphocytes and Plasma in Patients with EBV-Associated Diseases[J].Journal of Modern Laboratory Medicine,2017,32(06):15-18.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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淋巴细胞及血浆EB病毒DNA在EBV感染相关疾病中表达的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
15-18
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Expression of Epstein-Barr Virus DNA in Peripheral Blood Lymphocytes and Plasma in Patients with EBV-Associated Diseases
文章编号:
1671-7414(2017)06-015-04
作者:
喻 晶; 王 琳; 卢丽华; 吴夏枫; 刘晓翌; 张银汉
北京大学深圳医院检验科,广东深圳 518036
Author(s):
YU Jing; WANG Lin; LU Li-hua; WU Xia-feng; LIU Xiao-yi; ZHANG Yin-han
Department of Laboratory Medicine,Shenzhen Hospital of Beijing University,Guangdong Shenzhen 518036,China
关键词:
EB病毒DNA;  荧光定量PCR;  血浆;  外周血淋巴细胞;  EBV感染相关疾病
分类号:
R373.11; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 探讨外周血淋巴细胞和血浆中EB病毒DNA(EBV DNA)在EB病毒感染相关疾病中表达的差异。方法 收集112例疑似EB病毒感染相关疾病患者的全血样本,其中鼻咽癌14例,传染性单核细胞增多症(传单)16例,淋巴瘤22例,自身免疫性疾病23例,上呼吸道感染26例,肝功能异常11例,采用荧光定量PCR方法检测同一份样本淋巴细胞和血浆中EBV DNA的含量。结果 检测112例患者外周血淋巴细胞和血浆中的EBV DNA,其总的阳性率分别为83.0%(93/112),27.7%(31/112),差异具有统计学意义(卡方值χ2=60.02,P<0.01); 14例鼻咽癌患者外周血淋巴细胞和血浆EBV DNA的阳性率及其含量差异均无统计学意义(χ2=2.25,t=-1.04,均P>0.05); 而传染性单核细胞增多症、淋巴瘤、自身免疫性疾病、上呼吸道感染和肝功能异常患者血浆EBV DNA阳性率及其含量均明显低于外周血淋巴细胞,差异具有统计学意义(χ2=4.17～15.06,均P<0.05; t=3.94～10.45,均P<0.01)。结论 荧光定量PCR检测非鼻咽癌患者的外周血淋巴细胞的EBV DNA可能优于检测血浆的EBV DNA; 检测鼻咽癌患者外周血淋巴细胞和血浆EBV DNA无明显差异,可根据临床情况,选择合适的标本进行检测。
Abstract:
Abstract:Objective To explore the expression of Epstein-Barr virus DNA(EBV DNA)in the peripheral blood lymphocytes and plasma of patients with EBV-associated diseases.Methods The whole blood samples were collected from 112 patients with suspected EB virus infection diseases,including 14 cases of nasopharyngeal carcinoma(NPC),16 cases of infectious mononucleosis(IM),22 cases of lymphoma,23 cases of autoimmune disease,26 cases of upper respiratory tract infection,and 11 cases of abnormal liver function.The levels of EBV DNA in lymphocytes and plasma of the same sample weredetected by fluorescence quantitative PCR(FQ-PCR).Results The EBV DNA positive rates in lymphocytes and plasma of all 112 patients were 83.0%(93/112)and 27.7%(31/112)respectively,with statistically significant difference(χ2=60.02,P<0.01).The positive rate and the load of EBvirus DNA in lymphocytes and plasma of 14 patients with nasopharyngeal carcinoma(NPC)had no statistical difference(χ2=2.25,t=-1.04,all P>0.05).However,patients with lymphoma,infectious mononucleosis,upper respiratory tract infection,autoimmune disease or abnormal liver function,the positive rates and the concentration of EBV DNA in the plasma were dramatically lower than those in the peripheral blood lymphocytes,and the difference was statistically significant(χ2=4.17～15.06,all P<0.05; t=3.94～10.45,all P<0.01).Conclusion The detection of EB DNA in peripheral blood lymphocytes of non NPC patients by FQ-PCR might be better than that in plasma.There was no statistical difference between the detection of EBV DNA in lymphocytes and plasma of patients with nasopharyngeal carcinoma.Appropriate specimen type could be selected according to clinical consideration.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:喻 晶(1967-),女,医学硕士,主任技师,主要从事临床分子生物学研究,E-mail:jing_yu2004@aliyun.com。
更新日期/Last Update: 2017-12-26