[1]李腾达,刘 鹏,龙曙萍,等.隐球菌脑膜炎患者血清外泌体CD93表达增高及意义[J].现代检验医学杂志,2017,32(06):23-25.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　LI Teng-da,LIU Peng,LONG Shu-ping,et al.Elevated Expression of CD93 in Serous Exosomes from Patients with Cryptococcal Meningitis and Its Significance[J].Journal of Modern Laboratory Medicine,2017,32(06):23-25.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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隐球菌脑膜炎患者血清外泌体CD93表达增高及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
23-25
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Elevated Expression of CD93 in Serous Exosomes from Patients with Cryptococcal Meningitis and Its Significance
文章编号:
1671-7414(2017)06-023-03
作者:
李腾达1; 刘 鹏1; 龙曙萍1; 刘 云1; 黄元兰2; 张薇薇1; 郭 杰1; 谷明莉1; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1; LIU Peng1; LONG Shu-ping1; LIU Yun1; HUANG Yuan-lan2; ZHANG Wei-wei1;  GUO Jie1; GU Ming-li1; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
隐球菌脑膜炎;  外泌体;  CD93;  白介素-1β;  白介素-17
分类号:
R519.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 检测隐球菌脑膜炎患者血清中外泌体表达CD93的水平,进一步探究其临床意义。方法 实验组为2012年11月～2016年12月在上海长海医院和长征医院确诊的38例隐球菌脑膜炎患者的血清,诊断标准为脑脊液染色新生隐球菌阳性或培养阳性; 对照组为同期收集的38例健康人血清。血清外泌体的分离采用超高速离心法; 外泌体表面抗原的鉴定采用磁珠捕获结合流式细胞仪检测法; 血清中细胞因子用ELISA法进行蛋白定量。实验组与对照组检测值的比较采用两独立样本的t检验,两变量之间的相关性以Pearson系数表示。结果 流式结果表明,实验组血清外泌体中CD93的表达水平高于对照组为(79.11±19.31 vs 23.98±6.56)%,差异具有统计学意义(t=16.66,P<0.000 1)。IFN-γ在实验组与对照组血清中的表达水平为(39.78±10.77 vs 58.98±16.99)pg/ml,差异具有统计学意义(t=5.884,P<0.000 1); IL-17在实验组与对照组血清中的表达水平为(16.32±4.03 vs 3.11±0.87)pg/ml,差异具有统计学意义(t=19.75,P<0.000 1); IL-1β在实验组与对照组血清中的表达水平为(57.12±12.98 vs 13.45±4.78)pg/ml,差异具有统计学意义(t=19.46,P<0.000 1)。实验组血清中CD93+exosomes所占的百分比与IFN-γ,IL-17,IL-1β的蛋白表达量呈正相关(r=0.488,0.456,0.532,均P<0.01)。结论 隐球菌脑膜炎疾病过程中,CD93在外泌体的表达可能通过影响CD4+T细胞参与了该病病程。
Abstract:
Abstract:Objective To examine the expression of CD93 inserous exosomes from patients with cryptococcal meningitis,further explore its clinical significance.Methods The 38 experimental serum samples were from patients who received the diagnosis with cryptococcal meningitis in Changhai Hospital and Changzheng Hospital in Shanghai from November 2012to December 2016.The diagnosis standardization was that the cerebrospinal fluid dyeing was positive or the culturing was positive.The 38 controls were collected from the health individuals examined at the same time.The exosomes in serumwas separated by the ultracentrifuge method.Magnetic bead-capture combined with the flow cytometry method was used to identify antigens on the surface of exosomes.The protein level of cytokines in serum was quantified by ELISA method.The comparison of tested values from experimental and controlled groups was measured by two independent samples' t test,and the correlation between two variates was showed by Pearson coefficient.Results The resultsof flow cytometry showed that the expression of CD93 on exosomes in the serum samples from experimental group was higher than that in the controlled group,which was(79.11±19.31 vs 23.98±6.56)%,with difference in statistics(t=16.66,P<0.000 1).The expression levels of IFN-γ in serum samplefrom experimental and control groups were(39.78±10.77 vs 58.98±16.99)pg/ml,withdifference in statistics(t=5.884,P<0.000 1).The expression levels of IL-17 in serum sample from experimental and control groups were(16.32±4.03 vs 3.11±0.87)pg/ml,with difference in statistics(t=19.75,P<0.000 1).The expression levels of IL-1β in serum sample from experimental and control groups were(57.12±12.98 vs 13.45±4.78)pg/ml,with difference in statistics(t=19.46,P<0.000 1).The percentage of CD93+exosomes was positively correlative with IFN-γ,IL-17,IL-1β(r=0.488,0.456,0.532,P<0.01)in serum samples from the experimental group and there was statistical difference.Conclusion In the disease process of cryptococcalmeningitis,the expression of CD93 on exosomes might involve in the diseases'processes by affecting the CD4+T cells.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81501397,31500721,81501398,81401358,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士研究生,主要从事自身免疫研究,E-mail:tengdali@smmu.edu.cn。 刘 鹏(1982-),女,在读博士研究生,主要从事自身免疫研究,E-mail:ppliu23@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 谷明莉,女,检验技师,E-mail:mingligu@126.com,共同通讯作者。
更新日期/Last Update: 2017-12-26