[1]黄太宏,徐学静,胡 伟,等.慢性乙型肝炎患者外周血IL-37的表达水平及意义[J].现代检验医学杂志,2017,32(06):26-27,31.[doi:10.3969/j.issn.1671-7414.2017.06.001]
 HUANG Tai-hong,XU Xue-jing,HU Wei,et al.Expression and Clinical Significance of Blood IL-37 in Patients with Chronic HBV[J].Journal of Modern Laboratory Medicine,2017,32(06):26-27,31.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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慢性乙型肝炎患者外周血IL-37的表达水平及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
26-27,31
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Expression and Clinical Significance of Blood IL-37 in Patients with Chronic HBV
文章编号:
1671-7414(2017)06-026-03
作者:
黄太宏徐学静胡 伟王 森
南京大学医学院附属鼓楼医院检验科,南京 210008
Author(s):
HUANG Tai-hongXU Xue-jingHU WeiWANG Sen
Department of Clinical Laboratory,Nanjing Drum Tower Hospital Affiliated to Nanjing University Medicine School,Nanjing 210008,China
关键词:
慢性乙型肝炎 白细胞介素-37 炎性因子
分类号:
R512.62; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 研究慢性乙型肝炎(chronic hepatitis B,CHB)患者外周血血清中白细胞介素-37(IL-37)的表达水平,并探讨IL-37在CHB中的作用及意义。方法 通过ELISA检测35例CHB患者及35例健康对照者血清中IL-37的含量; 全自动生化仪及化学发光仪检测CHB患者血清中ALT,AST,HBsAg,HBeAg的含量,定量PCR及ELISA方法检测CHB患者血清中HBV DNA载量及细胞因子TNF-α,IL-10的表达水平,并分析IL-37含量分别与ALT,AST,HBsAg,HBeAg,HBV DNA,TNF-α及IL-10之间的相关性。结果 CHB患者组血清中IL-37的表达水平437.5±152.7 pg/ml,高于对照组(63.0±2.1 pg/ml),两组之间差异具有统计学意义(t=2.45,P=0.017)。Pearson相关性分析显示,IL-37的水平与AST,HBsAg,HBeAg,HBV DNA,TNF-α的水平不相关,而与ALT(r=0.406,P=0.019),IL-10(r=0.433,P=0.035)的含量呈正相关。结论 CHB患者血清中IL-37的水平高于健康对照,IL-37可能在肝损伤及乙肝慢性化形成中发挥重要作用。
Abstract:
Abstract:Objective To study the expression levels of IL-37 in serum in chronic HBV(CHB)patients,and investigate their clinical significance in CHB patients.Methods The expression levelsof IL-37 in serum from 35 CHB patients and 35 healthy controls were quantifiedby ELISA. This study also tested ALT,AST,HBsAg,HBeAg,HBV DNA,TNF-α and IL-10 in the serum of CHB patients,and also analyzed the relationship between the levels of IL-37 and ALT,AST,HBsAg,HBeAg,HBV DNA,TNF-α,IL-10 levels.Results The expression levels of IL-37 was increased inCHB patients 437.5±152.7 pg/ml compared to healthy donors(63.0±2.1 pg/ml).The difference between the two groups was statistically significant(t=2.45,P=0.017).The levels of IL-37 was found to correlate with ALT(r=0.406,P=0.019)and IL-10(r=0.433,P=0.035)but not AST,HBsAg,HBeAg,HBV DNA and TNF-α.Conclusion This study reveals thelevels of IL-37 in CHB patients was higher than healthy controls,and IL-37 may play an important role in liver damage and HBV chronic infection.

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备注/Memo

备注/Memo:
基金项目:国家自然基金青年基金资助(81601765)。 作者简介:黄太宏(1989-),男,学士,检验技师,主要从事免疫学研究,E-mail:1262117241@qq.com。 通讯作者:王 森,男,博士,主管技师,主要从事免疫学及病毒学研究,E-mail:slzw1985@163.com,
更新日期/Last Update: 2017-12-26