[1]孙 谦,周 辉,郭丽娜.血脂异常人群ApoE和SLCO1B1基因多态性及相关性研究[J].现代检验医学杂志,2017,32(06):28-31.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　SUN Qian,ZHOU Hui,GUO Li-na.Analysis of Dyslipidemia Correlation and Polymorphisms of ApoE and SLCO1B1 Gene in Dyslipidemia People[J].Journal of Modern Laboratory Medicine,2017,32(06):28-31.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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血脂异常人群ApoE和SLCO1B1基因多态性及相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
28-31
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Analysis of Dyslipidemia Correlation and Polymorphisms of ApoE and SLCO1B1 Gene in Dyslipidemia People
文章编号:
1671-7414(2017)06-028-04
作者:
孙 谦; 周 辉; 郭丽娜
中南大学湘雅医院检验科,长沙 410008
Author(s):
SUN Qian; ZHOU Hui; GUO Li-na
Department of Clinical Laboratory, Xiangya Hospital of Central South University,Changsha 410008,China
关键词:
ApoE基因;  SLCO1B1基因;  血脂异常;  多态性
分类号:
Q786; R446.112
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 分析湘雅医院血脂异常人群中ApoE和(SLCO1B1)基因型频率分布特征,探究ApoE和SLCO1B1基因型与各项血脂指标的相关性,为防治动脉粥样硬化性疾病提供依据。方法 收集湘雅医院2016年6月～2017年4月共87例血脂异常人群三酰甘油(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)数据,采用PCR荧光探针法检测87例血脂异常人群的ApoE和SLCO1B1基因型,分析基因频率分布特征并比较各基因型间各项血脂指标的差异。结果 87例血脂异常人群ApoE各基因型频率为:E2/E2型1.15%,E2/E3型13.79%,E2/E4型1.15%,E3/E3型56.32%,E3/E4型26.44%和E4/E4型1.15%,各等位基因频率分布以E3占比最高,为76.44%,E2与E4分别为8.62%和14.94%。E4表型组TC和LDL-C浓度大于E2,E3组,3组表型TG,TC,LDL-C,HDL-C浓度水平差异无统计学意义。SLCO1B1各基因型频率为:*1a/*1a型6.90%,*1a/*1b型36.78%,*1a/*15型13.79%,*1b/*1b型26.44%和*1b/*15型16.09%,各单体型频率分布以*1b占比最高,为52.87%,*1a与*15分别为32.18%和14.94%,未检测到*5型。SLCO1B1各组TG,TC,LDL-C和HDL-C浓度水平差异无统计学意义。结论 ApoE基因型频率分布不均,其多态性与血脂水平可能存在相关性; SLCO1B1基因频率分布存在种族差异性,其多态性与血脂水平不相关。临床医师可以根据ApoE和SLCO1B1基因型合理选择用药剂量,以防治冠状动脉粥样硬化性疾病。
Abstract:
Abstract:Objective To investigate the frequency distribution of ApoE and SLCO1B1 genotypes in abnormal blood lipid levels in Xiangya Hospital,explore the correlation of ApoE and SLCO1B1 genotypes with various indicators of dyslipidemia,and provide evidence for prevention and treatment of atherosclerotic diseases.Methods Blood lipid dataincluding TG,TC,LDL-C and HDL-C in 87 cases of dyslipidemia people in Xiangya Hospital from June in 2016 to April in 2017 were collected.The ApoE and SLCO1B1 genotypes were detected by PCR fluoroscopy in 87 cases of dyslipidemia.Thedistribution of gene frequency was analyzed and the differences of blood lipid indexes among the genotypes were compared.Results The frequencies of each ApoE genotype in 87 cases of dyslipidemia were E2/E2 1.15%,E2/E3 13.79%,E2/E4 1.15%,E3/E3 56.32%,E3/E4 26.44% and E4/E4 1.15%,respectively.The highest proportion of allele frequency was E3 with the percent of 76.44%,E2 and E4 occupied 8.62% and 14.94%,respectively.The concentration of LDL-C in E4 phenotype group was higher than that in E2 group and E3 group,and there was no significant difference in the levels of TG,TC,LDL-C and HDL-C.The frequencies of each SLCO1B1 genotype were *1a/*1a 6.90%,*1a/*1b 36.70%,*1a/*1513.79%,*1b/*1b 26.44% and *1b/*15 16.09%.The highest frequency of each haplotype was *1b with the percent of 52.87%,*1a and *15 occupied 32.18% and 14.94%,respectively,and no * 5 type was detected.There were no significant differences in TG,TC,LDL-C and HDL-C concentrations between SLCO1B1 groups.Conclusion ApoE gene frequency distribution was uneven.Its polymorphism be related with the lipid levels.The frequency distribution of SLCO1B1 gene hadracial differences,and its polymorphism was not related with lipid level.According to the the genotypes of ApoE and SLCO1B1,clinician can choose the rightdose of drug to prevent coronary atherosclerotic disease.

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备注/Memo

备注/Memo:
作者简介:孙 谦(1986-),男,硕士,主管技师,主要研究方向为分子个体化治疗,E-mail:sunq216@163.com。 通讯作者:周 辉(1966-),男,大学本科,副主任技师,主要研究方向:分子生物学,E-mail:xyzh66@126.com。
更新日期/Last Update: 2017-12-26