[1]石玉如,岳 莉,赵长城,等.血清壳多糖酶3样蛋白1检测在不同肝脏疾病中的应用价值[J].现代检验医学杂志,2017,32(06):39-42.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　SHI Yu-ru,YUE Li,ZHAO Chang-cheng,et al.Clinical Application Value of Chitinase-3-like Protein 1 in Patients with Different Liver Diseases[J].Journal of Modern Laboratory Medicine,2017,32(06):39-42.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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血清壳多糖酶3样蛋白1检测在不同肝脏疾病中的应用价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
39-42
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Clinical Application Value of Chitinase-3-like Protein 1 in Patients with Different Liver Diseases
文章编号:
1671-7414(2017)06-039-04
作者:
石玉如1; 岳 莉1; 赵长城1; 姚余有2; 戚应杰1
1.安徽省立医院感染病院,合肥 230022; 2.安徽医科大学,合肥 230032
Author(s):
SHI Yu-ru1; YUE Li1; ZHAO Chang-cheng1; YAO Yu-you2; QI Ying-jie1
1.Department of Infectious Diseases Hospital of Anhui Provincial Hospital, Hefei 230022,China; 2.Anhui Medical University,Hefei 230032,China
关键词:
壳多糖酶3样蛋白1;  肝脏疾病;  应用价值
分类号:
R575; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 探讨血清壳多糖酶3样蛋白1(CHI3L1)在慢性乙型肝炎(CHB)、肝硬化和肝癌患者中的应用价值。方法 收集安徽省立医院感染病院2016年1月～2017年2月经临床确诊的96例患者血清标本,其中CHB组28例,肝硬化组44例,肝癌组24例,另收集健康体检标本15例,采用酶联免疫吸附实验(ELISA)定量检测血清壳多糖酶3样蛋白1(CHI3L1)的浓度; 利用双抗体夹心免疫层析法检测高尔基蛋白(GP73); 采用化学发光法检测甲胎蛋白(AFP)。结果 CHB组、肝硬化组、肝癌组、健康体检组之间血清CHI3L1差异具有统计学意义(χ2=70.249,P<0.001),其中肝硬化组、肝癌组明显高于健康对照组(P<0.001); 各组之间血清GP73差异具有统计学意义(χ2=44.963,P<0.001),其中CHB组、肝硬化组、肝癌组显著高于健康对照组(P<0.05); 各组之间血清AFP差异具有统计学意义(χ2=57.606,P<0.001),其中肝癌组高于肝硬化组、CHB组、健康组(P<0.001)。将CHB组与肝硬化组中的GP73,CHI3L1,AFP做ROC曲线,CHI3L1的AUC为0.953(95%可信区间:0.902～1.000),敏感度和特异度分别为88.6%和92.9%,高于GP73和AFP。将肝硬化组与肝癌组中的AFP,CHI3L1,GP73做ROC曲线,AFP的AUC为0.930(95%可信区间:0.871～0.989),敏感度和特异度分别为75.0%和97.7%,高于CHI3L1与GP73。对AFP,GP73,CHI3L1在CHB,肝硬化和肝硬化中的相关性进行分析,AFP与GP73呈正相关(rs=0.491,P<0.001),AFP与CHI3L1呈正相关(rs=0.452,P<0.001),GP73与CHI3L1呈正相关(rs=0.554,P<0.001)。结论 血清CHI3L1在肝硬化中具有很好的诊断效能,优于GP73与AFP,而AFP在肝癌中的诊断价值要高于CHI3L1与GP73。
Abstract:
Abstract:Objctive To explore the clinical value of Chitinase-3-like protein 1(CHI3L1)in patients with chronic hepatitis B(CHB),liver cirrhosis and liver cancer.Methods 96 clinically diagnosed patients in Department of Infectious Diseases Hospital of Anhui ProvincialHospital from Jan 2016 to Feb 2017(28CHB,44livercirrhosis,24liver cancer,15healthy controls)were analyzed.The serum level of CHI3L1 was measured by enzyme-linked immunosorbentassay(ELISA).The Golgi protein(GP73)was tested by double-antibody sandwich immunochromatographic assay.The Alpha-fetoprotein(AFP)was tested by means of chemical luminescence.Results There were significant differences between the groups of chronic hepatitis B(CHB),liver cirrhosis,liver cancer and healthy controls on the CHI3L1 level(χ2=70.249,P<0.001).The CHI3L1 level of the liver cirrhosis group and the liver cancer group increased significantly compared with that of the healthy controls(P<0.001).The GP73 levels of these groups were significantly different(χ2=44.963,P<0.001).The GP73 levels of the CHB group,the liver cirrhosis group and the liver cancer group all increased significantly compared withthat of the healthy controls(P<0.05).The AFP levels of these groups weresignificantly different(χ2=57.606,P<0.001).The AFP level of the liver cancer group increased significantly compared with that of the CHB group,theliver cirrhosis group and the healthy controls(P<0.001).Based on the receiver operating characteristic(ROC)curve of CHI3L1,GP73 and AFP in the CHB group and the liver cirrhosisgroup,the Area Under roc Curve(AUC)of CHI3L1 was 0.953(95%CI:0.902～1.000),the sensitivity was 88.6%,and the specificity was 92.9%,which was higher than GP73 and AFP.Based on the ROC curve of CHI3L1,GP73 and AFP in the liver cirrhosis group and the liver cancer group,the AUC ofAFP was 0.930(95% CI:0.871～0.989),the sensitivity was 75.0%,and the specificity was 97.7%,which was higher than CHI3L1 and GP73.The correlation between the CHI3L1,GP73 and AFP in CHB,liver cirrhosis and liver cancer groups were analyzed.There was a positive correlation between AFP and GP73(rs=0.491,P<0.001),a positive correlation between AFP and CHI3L1(rs=0.452,P<0.001),a positive correlation between GP73 and CHI3L1(rs=0.554,P<0.001).Conclusion CHI3L1 is good at diagnosis of liver cirrhosism,better than GP73 and AFP.And AFP could be more beneficial in patients withliver cancer,better than CHI3L1 and GP73.

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相似文献/References:

[1]张巧娣,谢而付,凌 芸,等.壳多糖酶3样蛋白1与甲胎蛋白在肝细胞癌诊断中的比较[J].现代检验医学杂志,2017,32(01):45.[doi:10.3969/j.issn.1671-7414.2017.01.013]
　ZHANG Qiao-di,XIE Er-fu,LING Yun,et al.Comparative Diagnostic Value of Chitinase 3-Like 1 Protein and AFP in the Diagnosis of Hepatocellular Carcinoma[J].Journal of Modern Laboratory Medicine,2017,32(06):45.[doi:10.3969/j.issn.1671-7414.2017.01.013]

备注/Memo

备注/Memo:
作者简介:石玉如(1984-),女,研究生,主管检验技师,研究方向:临床感染性疾病的免疫与分子诊断,E-mail:290797374@qq.com。 通讯作者:戚应杰,男,副主任技师,研究方向:临床感染性疾病的实验室诊断与研究,E-mail:7yingjie@sina.com。
更新日期/Last Update: 2017-12-26