[1]徐少华,赵 静,朱 琳,等.HBeAg阳性慢性乙型肝炎患者大量输血后其血清标志物变化研究[J].现代检验医学杂志,2017,32(06):147-150.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　XU Shao-hua,ZHAO Jing,ZHU Lin,et al.Changes of Serum Markers in Patients with HBeAg Positive Chronic Hepatitis B after Massive Blood Transfusion[J].Journal of Modern Laboratory Medicine,2017,32(06):147-150.[doi:10.3969/j.issn.1671-7414.2017.06.001]
点击复制

HBeAg阳性慢性乙型肝炎患者大量输血后其血清标志物变化研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
147-150
栏目:
检验与临床
出版日期:
2017-12-25

文章信息/Info

Title:
Changes of Serum Markers in Patients with HBeAg Positive Chronic Hepatitis B after Massive Blood Transfusion
文章编号:
1671-7414(2017)06-147-04
作者:
徐少华1; 赵 静1; 朱 琳1; 杨 涛1; 谢晓霞1; 黄江华1; 应 军1; 郑崛村2
1.四川省三台县人民医院检验科,四川绵阳 621100; 2.成都医学院检验医学院,成都 610083
Author(s):
XU Shao-hua1; ZHAO Jing1; ZHU Lin1; YANG Tao1; XIE Xiao-xia1;  HUANG Jiang-hua1; YING Jun1; ZHENG Jue-cun2
1.Department of Clinical Laboratory,the People's Hospital of Santai County,Sichuan Mianyang 621100, China; 2.Department of Laboratory Medicine,Chengdu Medical College,Chengdu 610083,China
关键词:
乙型肝炎病毒;  乙肝病毒血清标志物;  输血治疗
分类号:
R512.62; R457.1
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 研究HBeAg阳性慢性乙型肝炎(chronic hepatitisB,CHB)患者接受输血治疗后其血清标志物的变化情况。方法 收集2015年1月1日～2016年1月1日在四川省川北医学院附属三台县人民医院曾在24 h内接受大量输血治疗的CHB患者26例,分别测定治疗前后患者血清中HBV浓度和HBV-DNA载量,对比治疗前后HBV和HBV-DNA的变化。结果 治疗后第1天患者HBsAg,抗-HBs和抗-HBc浓度相较治疗前差异有统计学意义(t=2.681,4.753,5.116,均P<0.01); 治疗后第3天患者HBsAg,抗-HBs和抗-HBc浓度相较治疗前依旧存在差异(t=1.681,2.209,3.118,均P<0.05); 治疗后第7天相较治疗前患者仅抗-HBc浓度存在差异(t=2.463,P<0.05); 患者HBeAg和HBV-DNA输血前后相比差异无统计学意义(t=0～1.132,均P>0.05); 输血治疗后,HBsAg浓度在第5天浓度为0.17±0.03 IU/ml,达到最低值,第7天浓度上升为387.50±31.89 IU/ml,达到最高值; HBsAb浓度逐渐下降,第7天浓度为1.51±5.98 mIU/mmol达到最低; HBeAg,HBeAb和HBcAb的浓度没有明显的变化。结论 HBeAg阳性CHB患者进行大量输血治疗后,短期内HBsAg,抗-HBs和抗-HBc可出现改变,HBeAg和HBV-DNA不受输血治疗的影响。
Abstract:
Abstract:Objective To investigate the changes of HBV markers in patients with HBeAg positive chronic hepatitis B(CHB)after a large number of blood transfusion treatment.Methods 26 patients with chronic HBV that were treated massive blood transfusion in 24h were collectedfrom Jan 1,2015 to Jan 1,2016.The HBV serum markers and HBV-DNA were measured and compared before and after treatment.Results TheHBsAg,anti-HBs and anti-HBc concentration in first day after treatment were different compared with before treatment(t=2.681,4.753 and 5.116,all P<0.01).The HBsAg,anti -HBs and anti-HBc concentration in third day aftertreatment exist differences compared with before treatment(t=1.681,2.209and 3.118,all P<0.05).The difference still exist in the seventh day after treatment compared with before treatment with only anti-HBc concentration(t=2.463,P<0.05).There was not difference of HBeAg and HBV-DNA before and after blood transfusion in patients(t=0～1.132,P>0.05).After transfusionthe concentration of HBsAg in the fifth day was the lowest concentration as0.17±0.03 IU/ml,the seventh day rose to 387.50±31.89 IU/ml,reaching thehighest value,and the concentration of HBsAb decreased gradually to minimum atthe seventh day that was 1.51±5.98 mIU/mmol,and the concentrations of HBeAg,HBeAb and HBcAb had no obvious change.Conclusion The HBsAg,anti-HBs and anti-HBc couldbe changed in patients with HBeAg positive CHB after massive transfusion therapy in short term.HBeAg and HBV-DNA were not affected by transfusion therapy.

参考文献/References:

[1] 张慧林,成昌慧,马效恩.分级诊疗制度的现状分析及对策思考[J].中国医院管理,2015,36(11):8-9. Zhang HL,Cheng CH,Ma XE.Status quo analysis and countermeasures thinking of health care grading system[J].Chinese Hospital Management,2015,36(11):8-9.
[2] 中华医学会肝病学分会.中华医学会感染病学分会.慢性乙型肝炎防治指南(2010年版)[J].中华传染病杂志,2011,29(2):65-80. Chinese Sociely of Hepatology,Chinese Medical Association,ChineseSociely of Infectious Diseases,Chinese Medical Association.The guidelines of prevention and treatment for chronic hepatitis B(2010 version)[J].Chinese Journal of Infectious Diseases,2011,29(2):65-80.
[3] 中华医学会肝病学分会,感染病学分会.慢性乙型肝炎防治指南[J].中华肝脏病杂志,2005,13(12):881-891. Chinese Sociely of Hepatology and Chinese Sociely of Infectious Diseases,Chinese Medical Association.The guidelines of prevention and treatment forchronic hepatitis B in infectious diseases branch[J].Chinese Journal of Infectious Diseases,2015,13(12):881-891.
[4] 谢 思,张卫云,陈建芸,等.HISCL-5000高敏发光全自动免疫分析仪检测感染性指标的临床性能评价研究进展[J].生物技术通讯,2016,27(1):142-146. Xie S,Zhang WY,Chen JY,et al.Advances in clinical preformance evaluation of automated immunoassay system HISCL-5000 to detect infectious index[J].Letters in Biotechnology,2016,27(1):142-146.
[5] 大量输血现状调研协作组.大量输血指导方案[J].中国输血杂志,2012,25(7):617-621. A Large Number of Transfusion Status Quo Investigation CollaborationGroup.A large number of blood transfusion guidelines[J].Chinese Journal ofBlood Transfusion,2012,25(7):617-621.
[6] Wu Y,Sato H,Suzuki T,et al.Involvement of c-Myc in the proliferationof MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2[J].Int J Mol Med,2015,35(3):815-820.
[7] 叶贤林,李 彤,孙淑君,等.核心抗体阳性合格献血者隐匿性乙肝病毒感染分子生物学特性及追踪结果的研究[J].中国输血杂志,2016,29(9):890-894. Ye XL,Li T,Sun SJ,et al.Characterization and follow-up study ofoccult hepatitis B virus infection in anti-HBC positive blood donors[J].Chin J Blood Transfusion,2016,29(9):890-894.
[8] 王 菲,许爱玲,辛霞霞,等.乙型肝炎患者血清HBeAg和抗-HBe与HBV DNA定量的关系[J].临床肝胆病杂志,2015,31(4):534-536. Wang F,Xu AL,Xin XX,et al.Relationship between E system of hepatitis B and HBV DNA quantification[J].J Clin Hepatol,2015,31(4):534-536.
[9] 邓 兰.CHB患者血清HBeAg水平与肝脏炎症活动度及纤维化分级的关系研究[J].实用临床医药杂志,2016,20(17):63-66. Deng L.Study on the relationship between serum HBeAg levels and liver inflammation activity and fibrosis grading in patients with CHB[J].Journalof Clinical Medicine in Practical,2016,20(17):63-66.
[10] 俸家富,黄文芳.现代临床检验诊断大全[M].成都:四川科学技术出版社,2013: 326-332. Feng JF,Huang WF.Modern clinical laboratory diagnostic monual[M].Chengdu:Sichuan Science and Technology Press,2013:326-332.
[11] 戴小波,唐文志.慢性乙型肝炎患者HBsAg,HBeAg及HBV-DNA定量结果的临床意义与相关性分析[J].中国输血杂志,2015,28(12):1498-1502. Dai XB,Tang WZ.Correlation and clinical significance between the quantitative results of HBsAg,HBeAg,HBV-DNA in chronic hepatitis B patients[J].Chinese Journal of Blood Transfusion,2015,28(12):1498-1502.
[12] 何紫琪,李从荣,尹 路.乙型肝炎病毒逆转录酶基因突变的临床意义[J].现代检验医学杂志,2016,31(4):83-86. He ZQ,Li CR,Yin L.Clinical significance of reverse transcriptase gene mutation in the hepatitis B virus[J].Journal of Modern Laboratory Medicine,2016,31(4):83-86. 收稿日期:2016-10-31 修回日期:2017-10-30

相似文献/References:

[1]李步荣,张彤,李丽华,等.CHB患者HBV基因型及耐药突变基因联合检测临床研究[J].现代检验医学杂志,2015,30(05):40.[doi:10.3969/j.issn.1671-7414.2015.05.012]
　LI Bu-rong,ZHANG Tong,LI Li-hua,et al.Clinical Study of Combined Detection about Hepatitis B Virus Genotypes and Drug Resistant Mutation Genes in Patients with Chronic Hepatitis B[J].Journal of Modern Laboratory Medicine,2015,30(06):40.[doi:10.3969/j.issn.1671-7414.2015.05.012]
[2]景媛媛,李锦,段勇,等.青年献血人群HBV/OBI感染者血清中IL-10表达水平的分析[J].现代检验医学杂志,2015,30(05):107.[doi:10.3969/j.issn.1671-7414.2015.05.033]
　JING Yuan-yuan,LI Jin,DUAN Yong,et al.Analysis of IL-10 in Serum of Blood Donors with OBI/HBV Infection among Young Adults[J].Journal of Modern Laboratory Medicine,2015,30(06):107.[doi:10.3969/j.issn.1671-7414.2015.05.033]
[3]谢小娟,李小侠,李芒会,等.孕产妇及其新生儿的HBV检测结果相关性分析[J].现代检验医学杂志,2015,30(02):80.[doi:10.3969/j.issn.1671-7414.2015.02.025]
　XIE Xiao-juan,LI Xiao-xia,LI Mang-hui,et al.Correlation Analysis of Maternal and Neonatal HBV Test Results[J].Journal of Modern Laboratory Medicine,2015,30(06):80.[doi:10.3969/j.issn.1671-7414.2015.02.025]
[4]何紫琪,李从荣,尹 路.乙型肝炎病毒逆转录酶基因突变的临床意义[J].现代检验医学杂志,2016,31(04):83.[doi:10.3969/j.issn.16717-414.2016.04.022]
　HE Zi-qi,LI Cong-rong,YIN Lu.Clinical Significance of Reverse Transcriptase Gene Mutations in the Hepatitis B Virus[J].Journal of Modern Laboratory Medicine,2016,31(06):83.[doi:10.3969/j.issn.16717-414.2016.04.022]
[5]王海宁,李万顺,孙巨军.乙肝病毒血清标志物、外周血T淋巴细胞与HBV-DNA的相关性分析[J].现代检验医学杂志,2016,31(04):113.[doi:10.3969/j.issn.16717-414.2016.04.032]
　WANG Hai-ning,LI Wan-shun,SUN Ju-jun.Correlation Study on Serum Markers of Hepatitis B Virus and Peripheral T Lymphocytes as Well as HBV-DNA in Patients with Hepatitis B[J].Journal of Modern Laboratory Medicine,2016,31(06):113.[doi:10.3969/j.issn.16717-414.2016.04.032]
[6]解 娟,刘文康,李 玲,等.化学发光分析法定量检测住院患者HBV血清标志物及临床意义[J].现代检验医学杂志,2017,32(03):40.[doi:10.3969/j.issn.1671-7414.2017.03.011]
　XIE Juan,LIU Wen-kang,LI Ling,et al.Clinical Implication of HBV Serum Markers Detected with Chemiluminescent Immunoassay in Inpatients of Xi’an Area[J].Journal of Modern Laboratory Medicine,2017,32(06):40.[doi:10.3969/j.issn.1671-7414.2017.03.011]
[7]张 孟a,李 倩b,周华君b,等.巢氏PCR扩增低拷贝HBV全基因组方法的建立及应用[J].现代检验医学杂志,2018,33(03):44.[doi:10.3969/j.issn.1671-7414.2018.03.013]
　ZHANG Menga,LI Qianb,ZHOU Hua-junb,et al.Establishment and Application of Nested PCR Amplification Method for the Whole Genome of HBV with the Low Copy[J].Journal of Modern Laboratory Medicine,2018,33(06):44.[doi:10.3969/j.issn.1671-7414.2018.03.013]
[8]梁湘辉,樊树洁.HBV感染者病毒载量与外周血白细胞VCS参数的相关性探讨[J].现代检验医学杂志,2018,33(06):115.[doi:10.3969/j.issn.1671-7414.2018.06.030]
　LIANG Xiang-hui,FAN Shu-jie.Correlative Study between Viral Load of Patients with HBV and VCS Parameters of Peripheral Blood Leukocytes[J].Journal of Modern Laboratory Medicine,2018,33(06):115.[doi:10.3969/j.issn.1671-7414.2018.06.030]
[9]刘　杨,彭道荣,霍　豆,等.慢性HBV 感染自然史不同阶段血清HBsAg 水平及与血清HBV-DNA 载量相关性的研究[J].现代检验医学杂志,2020,35(03):11.[doi:10.3969/j.issn.1671-7414.2020.03.003]
　LIU Yang,PENG Dao-rong,HUO Dou,et al.Study on Serum HBsAg Level and Its Correlation with Serum HBV-DNA Load in Different Phase of Natural History of Chronic HBV Infection[J].Journal of Modern Laboratory Medicine,2020,35(06):11.[doi:10.3969/j.issn.1671-7414.2020.03.003]
[10]泽汪格玛,杨建蓉,欧仕颖,等.慢性HBV感染患者血清CP，HNF1α与肝组织病理分级及分期的相关性研究[J].现代检验医学杂志,2022,37(03):177.[doi:10.3969/j.issn.1671-7414.2022.03.037]
　ZE Wanggema,YANG Jian-rong,OU Shi-ying,et al.Correlation between Serum CP, HNF 1α and Pathological Grading, Staging of Liver Tissues in Patients with Chronic HBV Infection[J].Journal of Modern Laboratory Medicine,2022,37(06):177.[doi:10.3969/j.issn.1671-7414.2022.03.037]

备注/Memo

备注/Memo:
作者简介:徐少华(1982-),男,学士,主管技师,主要从事临床基础检验研究,E-mail:eessee@163.com。 通讯作者:应 军,副主任技师,E-mail:tab969@163.com。 郑崛村,教授,博士生导师,E-mail:eehhee@163.com。
更新日期/Last Update: 2017-12-26