[1]王 华a,苍金荣b,王 曦,等.念珠菌类细菌样变异株基因特征初步研究[J].现代检验医学杂志,2018,33(01):19-21,24.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　WANG Huaa,CANG Jin-rongb,WANG Xi,et al.Preliminary Study on the Gene Characteristics of Oidiomycetes Mutant Strains Like Bacterial Morphology[J].Journal of Modern Laboratory Medicine,2018,33(01):19-21,24.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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念珠菌类细菌样变异株基因特征初步研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
19-21,24
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
Preliminary Study on the Gene Characteristics of Oidiomycetes Mutant Strains Like Bacterial Morphology
文章编号:
1671-7414(2018)01-019-04
作者:
王 华1a; 苍金荣1b; 王 曦2; 刘家云3; 任健康1b; 苏宝凤1b; 张利侠1b; 闫福堂1b; 归巧娣1b
1.陕西省人民医院,a.陕西省临床检验中心; b.检验科,西安 710068; 2.西安市红会医院,西安 710054; 3.空军军医大学西京医院,西安 710032
Author(s):
WANG Hua1a; CANG Jin-rong1b; WANG Xi2; LIU Jia-yun3; REN Jiang-kang1b; SU Bao-feng1b;  ZHANG LI-xia1b; YAN Fu-tang1b; GUI Qiao-di1b
1a.Shaanxi Provincial Center for Clinical Laborataries; 1b.Department of Laboratary Medicine,Shaanxi Provincial People's Hospital, Xi'an 710068,China; 2.Xi'an Red Cross Hospital,Xi'an 710054,China; 3.Xijing Hospital Affiliated to the Air Force Military Medical University,Xi'an 710032,China
关键词:
念珠菌类细菌样变异株;  基因;  PCR
分类号:
R531.3; R381
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 在对念珠菌发生变异后其形态、结构等生物学性状研究的基础上,进一步探讨念珠菌类细菌样变异株基因特征。方法 在低温、营养缺乏条件下诱导白色念珠菌标准菌株使其变异; 用不同浓度梯度的氟康唑等临床常用抗真菌药物诱导白色念珠菌标准菌株变异。采用煮沸法制备真菌基因模板,采用细菌保守基因16S RNA序列引物和真菌保守基因18S RNA序列引物分别扩增16S RNA和18S RNA序列,琼脂糖凝胶电泳,观察并记录结果。同时将细菌保守基因16S RNA扩增片段进行测序,所得序列进行BLAST比对分析。结果 念珠菌类细菌样变异株16S RNA序列均扩增阳性,而白色念珠菌标准株未出现相应的扩增条带。念珠菌类细菌样变异株18S RNA序列扩增除2号菌株有微弱的条带外,其余变异株均未扩增出目的条带,而白色念珠菌标准株则出现相应的扩增条带,表明念珠菌类细菌样变异株基因组中18S RNA序列所占比例不多或是因多次传代遗失。念珠菌类细菌样变异株16S RNA扩增片段经纯化后进行DNA序列测定,BLAST进行比对分析,发现念珠菌类细菌样变异株序列与数据库中细菌序列有较高的相似性。结论 念珠菌类细菌样变异株基因组含有细菌保守基因及少量真菌保守基因,具有原核生物学性状的念珠菌类细菌样变异株是源于真核生物的念珠菌。此研究在生物进化特别是在原核细胞与真核细胞的进化联系上有非常重要的意义。
Abstract:
Abstract:Objective To further explore the genetic characteristics of oidiomycetes mutant strains like bacterial morphology on the basisof the study on morphology and structure of mutated candida.MethodsThe standard strains of candida albicans were induced by low temperature and under the condition of low temperature and nutrient deficiency.Variation of standard strains of Candida albicans were induced by clinical antifungaldrugs such as fluconazole with different concentration gradient.Fungal gene template was prepared by boiling method,sequences of 16SRNA and 18SRNA were amplified using bacteria conservative gene sequence of 16SRNA and fungal conserved gene sequence of 18SRNA,and observed and recorded the results agarose gel electrophoresis.At the same time,the amplified fragment of bacterial conservative gene 16SRNA was sequenced,and the sequence was analyzed by BLAST comparison.Results the 16SRNA sequences of candida variant were amplifiedpositive,while the standard strain of candida albicans did not show the corresponding amplification band.Except 2 strains which showed a faint band,the other variants of the 18SRNA sequences did not amplified the target band,while thestandard strains of candida albicans showed a corresponding amplification bands.Suggested that proportion of 18SRNA sequences in the genome of oidiomycetes mutant strains like bacterial morphology was not much even lack.The 16SRNA fragments amplified of oidiomycetes mutant strains like bacterial morphology did determination of DNA sequence after purification.BLAST comparison analysis,it was found that sequence of oidiomycetes mutant strains like bacterial morphology hadhigher similarity with bacterial sequences in the database.Conclusion Oidiomycetes mutant strains like bacterial morphology contained bacterial and a small amount of fungus conservative gene.Oidiomycetes mutant strains like bacterial morphology with original nuclear biological character are ones from eukaryotes.This study is great significance in biological evolution,especially in the evolution of prokaryotic cells and eukaryotic cells.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(S2014JC8839)。 作者简介:王 华(1963-),男,主任检验师,主要从事临床微生物学研究。 通讯作者:苍金荣(1962-),女,副主任检验师,E-mail:cang1963@163.com。
更新日期/Last Update: 2018-02-07