[1]李腾达,黄元兰,龙曙萍,等.CD36在隐球菌脑膜炎患者外周血单个核细胞中表达升高及其临床意义[J].现代检验医学杂志,2018,33(01):56-58.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　LI Teng-da,HUANG Yuan-lan,LONG Shu-ping,et al.High Expression of CD36 in Peripheral Blood Mononuclear Cells from Patients with Crytococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2018,33(01):56-58.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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CD36在隐球菌脑膜炎患者外周血单个核细胞中表达升高及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
56-58
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
High Expression of CD36 in Peripheral Blood Mononuclear Cells from Patients with Crytococcal Meningitis and Its Clinical Significance
文章编号:
1671-7414(2018)01-056-03
作者:
李腾达1; 黄元兰2; 龙曙萍1; 刘 云1; 刘 鹏1; 张薇薇1; 郭 杰1; 谷明莉1; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1; HUANG Yuan-lan2; LONG Shu-ping1; LIU Yun1; LIU Peng1; ZHANG Wei-wei1;  GUO Jie1; GU Ming-li1; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
隐球菌脑膜炎;  CD36;  干拢素-γ;  转化生长因子-β
分类号:
R519.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 探究CD36在隐球菌脑膜炎患者外周血单个核细胞(PBMCs)中的表达水平及其临床意义。方法 以2013年9月～2017年2月于上海长海医院和长征医院确诊的36例隐球菌脑膜炎患者外周血为实验组,以同期体检的36例健康者外周血作为对照组,采用密度梯度离心法进行PBMCs的分离,以qRT-PCR法检测PBMCs中CD36,TLR4的相对mRNA表达水平,以ELISA法检测血浆中TNF-α等细胞因子的表达水平,实验组与对照组检测值的比较采用两独立样本t检验,对实验组PBMCs中CD36的mRNA相对表达水平与TNF-α等细胞因子进行Pearson相关性分析。结果 实验组PBMCs中CD36的mRNA相对表达水平为2.01±0.63,对照组为1.49±0.47,两组比较差异具有统计学意义(t=3.969,P=0.000 2)。Pearson分析结果显示实验组CD36与血浆中IFN-γ蛋白表达水平呈正相关(r=0.384,P<0.05),与TGF-β呈负相关(r=-0.487,P<0.005)。结论 CD36可能通过影响IFN-γ,TGF-β等因子的表达参与隐球菌脑膜炎的免疫病理过程,是该病监测和治疗潜在的靶点。
Abstract:
Abstract:Objective To explore the expression level of CD36 in peripheral blood mononuclear cells(PBMCs)from patients with crytococcal meningitis and its clinical significance.Methods The experimental group was the peripheral blood samples from 36 patients diagnosed as crytococcal meningitis in Changhai Hospital and Changzheng Hospital in Shanghai from September 2013 to February 2017,while the control group was the peripheralblood samples from 36 health individuals examined at the same time.PBMCs wereseparated by density gradient centrifugation method,the relative mRNA expression of CD36,TLR4 in PBMCs was tested by qRT-PCR,the expression level of cytokines such as TNF-α in plasma was tested by ELISA,the comparison of the examinedvalues of experimental and control groups performed by two independent samples' t test,Pearson correlation analysis was performed on the relative mRNA expression of CD36 in PBMCs and cytokines such as TNF-α in experimental group.Results The relative mRNA expression of CD36 in PBMCs ofexperimental group was 2.01±0.63,the control was 1.49±0.47,and there was statistical difference(t=3.969,P=0.000 2).Pearson analysis results showed that in experimental group CD36 was positively related with theprotein expression level of IFN-γ in plasma(r=0.384,P<0.05)and negatively related with TGF-β(r=-0.487,P<0.005).Conclusion CD36 might involve in the immunopathology process of crytococcal meningitic by influencing the expression of cytokines such as IFN-γ,TGF-β,and wasthe potential target for monitoring and treating this disease.

参考文献/References:

[1] Williamson PR,Jarvis JN,Panackal AA,et al.Cryptococcal meningitis:epidemiology,immunology,diagnosis and therapy[J].Nature Reviews Neurology,2017,13(1):13-24.
[2] Rohatgi S,Pirofski LA.Host immunity to Cryptococcus neoformans[J].Future Microbiology,2015,10(4):565-581.
[3] Abumrad NA,Goldberg IJ.CD36 actions in the heart:Lipids,calcium,inflammation,repair and more?[J].Biochimica Biophysica Acta,2016,1861(10):1442-1449.
[4] Ren Y.Peroxisome proliferator-activator receptor ga-mma:A link betweenmacrophage CD36 and inflammation in malaria infection[J].PPAR Research,2012,2012(9):640769.
[5] Blanchet C,Jouvion G,Fitting C,et al.Protective or deleterious role of scavenger receptors SR-A and CD36 on host resistance to Staphylococcus aureus depends on the site of infection[J].PLoS One,2014,9(1):e87927.
[6] 李腾达,龙曙萍,徐贵霞,等.CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及相关细胞因子的影响与临床意义[J].现代检验医学杂志,2016,31(5):38-41. Li TD,Long SP,Xu GX,et al.Affection of CD26/DPP4 on CD4+T cells and relative cytokines in patients with crytococcal meningitis and itsclinical significance[J].J Mod Lab Med,2016,31(5):38-41.
[7] Gowda NM,Wu X,Kumar S,et al.CD36 contributes to malaria parasite-induced pro-inflammatory cytokine production and NK and T cell activation by dendritic cells[J].PLoS One,2013,8(10):e77604.
[8] Rios FJ,Ferracini M,Pecenin M,et al.Uptake of oxLDL and IL-10 production by macrophages requires PAFR and CD36 recruitment into the same lipid rafts[J].PLoS One,2013,8(10):e76893. 收稿日期:2017-06-20 修回日期:2017-11-25

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556, 81601406,81471605,81501397,31500721,81501398,81401358,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士研究生,主要从事感染免疫与自身免疫研究,E-mail:tengdali@smmu.edu.cn。 黄元兰(1983-),女,博士,主要从事真菌感染与免疫机制研究,E-mail: huang_yuanlan@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 谷明莉,女,检验技师,E-mail: mingligu@126.com,共同通讯作者。
更新日期/Last Update: 2018-02-07