[1]程 涛,黄 刚.血清miRNA-21,miRNA-126表达在诊断PCI术后冠状动脉再狭窄的临床应用价值[J].现代检验医学杂志,2018,33(01):59-62，66.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　CHENG Tao,HUANG Gang.Clinical Value of Serum miRNA-21 and miRNA-126 Expression in Diagnosis of the Coronary Artery Restenosis after PCI[J].Journal of Modern Laboratory Medicine,2018,33(01):59-62，66.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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血清miRNA-21,miRNA-126表达在诊断PCI术后冠状动脉再狭窄的临床应用价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
59-62，66
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
Clinical Value of Serum miRNA-21 and miRNA-126 Expression in Diagnosis of the Coronary Artery Restenosis after PCI
文章编号:
1671-7414(2018)01-059-05
作者:
程 涛1; 黄 刚2
1.泾阳县医院检验科,陕西泾阳 713700; 2.陕西中医药大学附属医院检验科,陕西咸阳 712000
Author(s):
CHENG Tao1; HUANG Gang2
1.Departerment of Clinical Laboratory,the Hospital of Jingyang Country,Shaanxi Jingyang 713700,China; 2.Department of Clinical Laboratory,the Affiliated Hospital of Shaanxi University of Chinese Traditional Medicine,Shaanxi Xianyang 712000,China
关键词:
急性冠脉综合征;  冠状动脉介入术;  冠状动脉再狭窄;  血管内超声;  miRNA-21;  miRNA-126
分类号:
R531.3; R381
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 研究血清miRNA-21,miRNA-126检测在诊断经皮冠状动脉介入术(PCI)术后冠状动脉再狭窄(ISR)的临床应用。方法 收集82例在泾阳县医院和陕西中医药大学附属医院接受PCI治疗的急性冠脉综合征(ACS)患者(狭窄组30例,未狭窄组52例)及33例体验健康者(对照组)的血清及临床资料,采用real-time qPCR技术检测血清中miRNA-21和miRNA-126的相对表达量,比较分析其在各组中的变化。应用血管内超声(intravascular ultrasound,IVUS)来评价PCI术后再狭窄的发生。结果 血清miRNA-21和miRNA-126在对照组、未狭窄组和狭窄组中的表达量分别为(0.22±0.03,0.43±0.08,0.92±0.17)和(0.87±0.15,0.49±0.10,0.23±0.04)。与对照组比较,血清miRNA-21在狭窄组与未狭窄组的表达量明显增高,而miRNA-126表达水平则显著降低,差异均有统计学意义(P=0.000)。狭窄组与未狭窄组比较miRNA-21表达水平明显增高,而miRNA-126表达水平则显著降低,差异均有统计学意义(P=0.000)。IVUS结果显示,狭窄组的斑块面积(PLA)与未狭窄组比较显著增高,而最小管腔面积(MLA)显著降低,其差异均有统计学意义(P=0.000)。两标志物在狭窄组的表达量具有负相关性(r=-0.919,P<0.01)。狭窄组中miRNA-126和miRNA-21水平分别与PLA和MLA具有相关性(r=-0.945,0.926,P<0.01)和(r=0.933,-0.917,P<0.01)。结论 血清miRNA-21和miRNA-126水平检测可用于急性冠脉综合征疾病PCI术后冠状动脉再狭窄的诊断以及预测,可能作为该疾病时的基因检测指标之一。
Abstract:
Abstract:Objective To study the clinical application ofserum miRNA-21 and miRNA-126 in diagnosis of the coronary artery restenosis after percutaneous coronary intervention(PCI).Methods Theserum and clinical data of 82 cases of acute coronary syndrome(ACS)patientstreated with PCI from the Hospital of Jingyang Country and the Affiliated Hospital of Shaanxi University of Chinese Traditional Medicine(stenosis group consisted of 30 cases,non-stenosis group consisted of 52 cases),and healthy persons(control group consisted of 33 cases)were collected.The relative expressionlevels of serum miRNA-21 and miRNA-126 were detected using real-time qPCR technology in the comparative analysis for their changes in the groups.Intravascular ultrasound(IVUS)was used to evaluate the occurrence of restenosis after PCI.Results Serum miRNA-21 and miRNA-126 levels in the control group,the non-stenosis group and the stenosis group were,,(0.22±0.03,0.43±0.08,0.92±0.17)and(0.87±0.15,0.49±0.10,0.23±0.04),respectively.Compared with the control group,the expression level of serum miRNA-21 in the stenosis group and the non stenosis group were significantly increased,while the miRNA-126 levels was significantly decreased(P=0.000),thedifferences were statistically significant.Compared with the non stenosis group,the level of miRNA-21 in the stenosis group was significantly increased,while the level of miRNA-126 was significantly decreased(P=0.000),the differences were statistically significant.The results of IVUS showed that the plaquearea(PLA)of the patients in the stenosis group was significantly higher thanthat in the non stenosis group,while the minimal luman area(MLA)was significantly decreased,and the differences were statistically significant(P=0.000).There was a negative correlation between the expression of two markers in thestenosis group(r=-0.919,P<0.01).The levels of miRNA-126 and miRNA-21in the stenosis group were correlated with PLA and MLA(r=-0.945,0.926,P<0.01)and(r=0.933,-0.917,P<0.01).Conclusion The detection of serum miRNA-21 and miRNA-126 levels can be used in diagnosis and prediction for the coronary artary restenosis of ACS after PCI,as the gene detection index of the disease.

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备注/Memo

备注/Memo:
作者简介:程 涛(1982-),男,大学本科,主管检验师,研究方向:心血管疾病的基因诊断,E-mail:3248089878@qq.com。 通讯作者:黄 刚(1979-),男,大学本科,主管检验师,研究方向:心血管疾病的基因诊断,E-mail:3436309161@qq.com。
更新日期/Last Update: 2018-02-07