[1]李腾达,龙曙萍,黄元兰,等.自身免疫性胰腺炎患者血清外泌体中CD69表达升高及意义[J].现代检验医学杂志,2018,33(02):8-10.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　LI Teng-da,LONG Shu-ping,HUANG Yuan-lan,et al.Elevated Expression of CD69 in Serum Exosomes from Patients with Autoimmune Pancreatitis and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(02):8-10.[doi:10.3969/j.issn.1671-7414.2018.02.001]
点击复制

自身免疫性胰腺炎患者血清外泌体中CD69表达升高及意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
8-10
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Elevated Expression of CD69 in Serum Exosomes from Patients with Autoimmune Pancreatitis and Its Significance
文章编号:
1671-7414(2018)02-008-03
作者:
李腾达1; 龙曙萍1; 黄元兰2; 刘 鹏1; 张薇薇1; 郭 杰1; 刘 云1; 谷明莉1; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1; LONG Shu-ping1; HUANG Yuan-lan2; LIU Peng1; ZHANG Wei-wei1; GUO Jie1;  LIU Yun1; GU Ming-li1; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
自身免疫性胰腺炎;  外泌体;  CD69;  IL-4;  TGF-β
分类号:
R576; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 探究自身免疫性胰腺炎(AIP)患者血清外泌体中CD69的表达情况,初步探讨其对疾病病理过程的影响。方法 收集2012年10月～2016年12月来长海医院就诊的35例AIP患者血清和同期就诊的35例健康个体血清,分别作为实验组和对照组。以ELISA法检测血清中的细胞因子浓度,以磁珠结合抗体的方法在流式细胞仪中检测血清中外泌体表面分子的表达情况,两组之间计量资料的比较采取两独立样本t检验,两变量之间的相关性以Pearson系数表示。结果 流式细胞仪分析显示,实验组血清中外泌体CD69表达水平高于对照组(85.76±19.45 vs 17.01±5.89)%,差异有统计学意义(t=20.01,P<0.0001); 实验组中CD69+的外泌体所占百分比与血清中IL-4,IFN-γ,TGF-β表达量呈正比(r=0.456,0.678,0.548,均P<0.05),CD69-的外泌体所占百分比与血清中IL-4,IFN-γ,IL-10,TGF-β呈反比(r=-0.589,-0.399,-0.784,-0.657,均P<0.05)。结论CD69在外泌体的表达可能通过影响CD4+T细胞功能参与了AIP病程,是该病潜在的监测指标和治疗靶点。
Abstract:
Abstract:Objective To study the expression of CD69 in exosomes existed in serum from patients with autoimmune pancreatitis(AIP)and primarily discuss its affection on the pathology of disease progression.Methods Serum samples from 35 cases diagnosed as AIP and 35 healthy individuals examined at the same time were collected during October 2012 to December 2016 in Changhai Hospital,they were defined as experimental and control groups,separately.The cytokine levels in serum were measured by ELISA.The expression of surfaced molecules in exosomes existed in serum was tested by magnetic bead-conjunct-antibody method in flow cytometry.The comparison between the two groups' measurement data was measured by two independent samples' ttest,correlation between two variables was showed by Pearson coefficient.Results The flow cytometry analysis showed that the expressionlevel of CD69 in serum exosomes in experiment and control groups were(85.76±19.45 vs 17.01±5.89)%,with statistical difference(t=20.01,P<0.0001).CD69+ exosomes in experiment group were positively related with serum IL-4,IFN-γ and TGF-β(r=0.456,0.678,0.548,all P<0.05),while CD69-exosomes were negatively related with serum IL-4,IFN-γ,IL-10 and TGF-β(r=-0.589,-0.399,-0.784,-0.657,all P<0.05).Conclusion The expression of CD69 in exosomes might participate in AIP progression through influencing the function of CD4+T cell, and it was the potential examined biomarker and therapeutic target.

参考文献/References:

[1] Madhani K,Farrell JJ.Autoimmune pancreatitis:An update on diagnosis and management[J].Gastroenterology Clinics of North America,2016,45(1):29-43.
[2] Zen Y.The pathology of IgG4-related disease in the bile duct and pancreas[J].Seminars in Liver Disease,2016,36(3):242-256.
[3] Okazaki K,Sumimoto K,Mitsuyama T,et al.Abnormal immunity in IgG4-related autoimmune pancreatitis[J].Japanese Journal of Clinical Immunology,2014,37(1):11-18.
[4] Nuzhat Z,Kinhal V,Sharma S,et al.Tumour-derived exosomes as a signature of pancreatic cancer-liquid biopsies as indicators of tumour progression[J].Oncotarget,2017,8(10):17279-17291.
[5] Bonjoch L,Casas V,Carrascal M,et al.Involvement of exosomes in lung inflammation associated with experimental acute pancreatitis[J].The Journal ofPathology,2016,240(2):235-245.
[6] Costa-Silva B,Aiello NM,Ocean AJ,et al.Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver[J].Nature Cell Biology,2015,17(6):816-826.
[7] Radulovic K,Rossini V,Manta C,et al.The early activation marker CD69regulates the expression of chemokines and CD4 T cell accumulation in intestine[J].PLoS One,2013,8(6):e65413.
[8] Radenkovic M,Silver C,Arvastsson J,et al.Altered regulatory T cell phenotype in latent autoimmune diabetes of the adults(LADA)[J].Clinical and Experimental Immunology,2016,186(1):46-56.
[9] Yang Q,Wang J,Liu R,et al.Amelioration of concanavalin A-induced autoimmune hepatitis by magnesium isoglycyrrhizinate through inhibition of CD4(+)CD25(-)CD69(+)subset proliferation[J].Drug Design,Development and Therapy,2016,10:443-453.
[10] Bischof J,Muller S,Borufka L,et al.Quantitative trait locusanalysis implicates CD4(+)/CD44high memory T cells in the pathogenesis of murineautoimmune pancreatitis[J].PLoS One,2015,10(9):e0136298.
[11] 李腾达,龙曙萍,徐贵霞,等.CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及相关细胞因子的影响与临床意义[J].现代检验医学杂志,2016,31(5):38-41. Li TD,Long SP,Xu GX,et al.Affection of CD26/DPP4 on CD4+T cells and relative cytokines in patients with crytococcal meningitis and its clinical significance[J].Journal of Modern Laboratory Medicine,2016,31(5):38-41.
[12] Melo SA,Luecke LB,Kahlert C,et al.Glypican-1 identifies cancer exosomes and detects early pancreatic cancer[J].Nature,2015,523(7559):177-182.

相似文献/References:

[1]李腾达,龙曙萍,黄元兰,等.CD69+CD4+CD25-T在自身免疫性胰腺炎患者外周血中表达升高及临床意义[J].现代检验医学杂志,2017,32(04):29.[doi:10.3969/j.issn.1671-7414.2017.04.008]
　LI Teng-da,LONG Shu-ping,HUANG Yuan-lan,et al.Elevated Expression of CD69+CD4+CD25-T Cells in Peripheral Blood from Patients with Autoimmune Pancreatitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(02):29.[doi:10.3969/j.issn.1671-7414.2017.04.008]
[2]李腾达,刘 鹏,龙曙萍,等.隐球菌脑膜炎患者血清外泌体CD93表达增高及意义[J].现代检验医学杂志,2017,32(06):23.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　LI Teng-da,LIU Peng,LONG Shu-ping,et al.Elevated Expression of CD93 in Serous Exosomes from Patients with Cryptococcal Meningitis and Its Significance[J].Journal of Modern Laboratory Medicine,2017,32(02):23.[doi:10.3969/j.issn.1671-7414.2017.06.001]
[3]李腾达,刘 鹏,龙曙萍,等.表面增强拉曼光谱技术检测隐球菌脑膜炎患者血清中CD93+外泌体及其意义[J].现代检验医学杂志,2018,33(04):4.[doi:10.3969/j.issn.1671-7414.2018.04.002]
　LI Teng-da,LIU Peng,LONG Shu-ping,et al.Detection of Serum CD93+Exosomes in Patients with Cryptococcal Meningitis by Surface-Enhanced Raman Scattering Technology and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(02):4.[doi:10.3969/j.issn.1671-7414.2018.04.002]
[4]范维肖,刁艳君,马越云,等.超速离心法与QIAGEN膜亲和柱法提取前列腺癌细胞培养上清外泌体的方法学比较[J].现代检验医学杂志,2019,34(03):6.[doi:10.3969/j.issn.1671-7414.2019.03.002]
　FAN Wei-xiao,DIAO Yan-jun,MA Yue-yun,et al.Comparison of Ultracentrifugation and Membrane Based-Affinity ColumnMethods in Exosome Isolation from Supernatants of Prostate Cancer Cells[J].Journal of Modern Laboratory Medicine,2019,34(02):6.[doi:10.3969/j.issn.1671-7414.2019.03.002]

备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士生,主要从事感染免疫研究,tengdali@smmu.edu.cn。 龙曙萍(1994-),女,在读硕士生,主要从事感染免疫研究,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。谷明莉,女,检验技师,E-mail: mingligu@126.com,共同通讯作者。
更新日期/Last Update: 2018-04-16