[1]刘宇豪,陈 晨,次仁曲珍,等.耐碳青霉烯类肠杆菌科细菌耐药基因分析[J].现代检验医学杂志,2018,33(02):55-57.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　LIU Yu-hao,CHEN Chen,CIREN Qu-zhen,et al.Drug Sensitivity and Resistance Gene of Carbapenem Resistant Enterobacteriaceae[J].Journal of Modern Laboratory Medicine,2018,33(02):55-57.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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耐碳青霉烯类肠杆菌科细菌耐药基因分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
55-57
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Drug Sensitivity and Resistance Gene of Carbapenem Resistant Enterobacteriaceae
文章编号:
1671-7414(2018)02-055-03
作者:
刘宇豪1; 陈 晨1; 次仁曲珍2; 周俊英2; 3
1.武汉大学,武汉 430072; 2.西藏山南妇幼保健院,西藏山南 856000; 3.武汉大学中南医院,武汉 430071
Author(s):
LIU Yu-hao1; CHEN Chen1; CIREN Qu-zhen2; ZHOU Jun-ying2; 3
1.Wuhan University,Wuhan 430072,China; 2.Shannan Maternal and Child Health Care Hospital in Tibet,Tibet Shannan 856000,China; 3.Zhongnan Hospital of Wuhan University,Wuhan 430071,China
关键词:
耐碳青霉烯类肠杆菌科细菌;  改良Hodge试验;  耐碳青霉烯酶耐药基因
分类号:
R378.2; Q781
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 对武汉大学中南医院耐碳青霉烯类肠杆菌科细菌(Carbapenem resistant Enterobacteriaceae,CRE)进行耐药性及基因分析,为CRE感染提供依据。方法 采用WHONET5.6软件对CRE菌株进行耐药分析; 改良Hodge试验对53株CRE菌株采用亚胺培南、厄他培南和美洛培南三种药敏纸片进行表型筛选; 对31株CRE进行耐药基因分析。结果 53株CRE中,肺炎克雷伯菌34株占64.15%,大肠埃希菌12株占22.64%,阴沟肠杆菌7株占13.21%; Hodge试验阳性菌株有31株,占58.49%(31/53),亚胺培南、厄他培南和美罗培南三种药敏纸片的Hodge试验结果一致; 31株CRE中,扩增出blaKPC基因的菌株有29株,占93.55%,blaNDM基因有9株,占29.03%,blaKPC和blaNDM两种基因同时含有的菌株是8株,占25.81%,未扩增出blaIMP,blaOXA和blaVIM耐药基因。结论 31株耐碳青霉烯类肠杆菌主要耐药机制携带KPC和NDM型碳青霉烯酶基因; 重症医学科和呼吸内科病房检出的CRE主要是肺炎克雷伯菌,其耐药基因型主要是KPC型,干部病房CRE主要是大肠埃希菌,其耐药基因型主要是KPC和NDM的混合型。
Abstract:
Abstract:Objective To analyze the resistance genes of carbapenem resistant Enterobacteriaceae(CRE)from Zhongnan Hospital of WuhanUniversity,so as to provide evidence for CRE infection.Methods WHONET 5.6 software was used to analyze the resistance of carbapenem resistant Enterobacteriaceae isolated.Phenotype screening of 53 CRE strains taken with the modified Hodge test by three drug susceptibility slip to imipenem,meropenem and ertapenem,and analysed resistance gene analysis of 31 CRE strains phenotype screening.Results In 53 CRE strains,34 strains of Klebsiella pneumoniae,accounting for 64.15%,12 strains of Escherichia coli,accounting for 22.64%,7 strains of Enterobacter cloacae accounted for 13.21%,and there were 31 strains of Hodge test positive strains,accounting for 58.49%(31/53).The Hodge test results of three kinds of drug sensitive paper of imipenem,meropenem and ertapenem were consistent.In 31 strainsof CRE,29 strains of blaKPC gene were amplified,accounting for 93.55%,9 strains of blaNDM gene,accounting for 29.03%,contained simaltaneous and two genes of blaKPC and blaNDM were 8 strains,accounting for 25.81%,the resistance genes of BlaIMP,blaOXA,and blaVIM were not amplified.Conclusion Resistance to carbapenems was mainly due to the production of KPC and NDM in Enterobacteriaceae of 31 strains.The CRE was detected in the IntensiveCare Unit and Respiratory ward for mainly Klebsiella pneumoniae,and the main genotype was KPC.The CRE was detected in the Cadre ward mainly Escherichiacoli,and the drug-resistant genotype was mainly a mixed type of KPC and NDM.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:湖北省卫生和计划生育委员会科研项目,WJ2017H0021。 作者简介:刘宇豪(1995-),男,大学本科,E-mail:355149859@qq.com。 通讯作者:周俊英,女,副主任技师,主要从事病原微生物的临床与教学工作,现在西藏山南妇幼保健院,E-mail:1348548858@qq.com。
更新日期/Last Update: 2018-04-16