[1]高 飞,唐 晶,李景岗,等.LD BC-Ⅰ全自动血细胞图像分析仪对外周血有核细胞分类能力的验证评价[J].现代检验医学杂志,2018,33(02):86-90.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　GAO Fei,TANG Jing,LI Jing-gang,et al.Evaluation of LD BC-Ⅰ Blood Cell Image Automatic Analyzer in Analysis of the Nucleated Cells in Peripheral Blood Smear[J].Journal of Modern Laboratory Medicine,2018,33(02):86-90.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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LD BC-Ⅰ全自动血细胞图像分析仪对外周血有核细胞分类能力的验证评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
86-90
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Evaluation of LD BC-Ⅰ Blood Cell Image Automatic Analyzer in Analysis of the Nucleated Cells in Peripheral Blood Smear
文章编号:
1671-7414(2018)02-086-05
作者:
高 飞1; 唐 晶2; 李景岗1; 张 凤1; 龙 莉2; 曹得华2; 汪 键2
1.福建医科大学附属协和医院血液病研究所,福州 350001; 2.武汉兰丁医学高科技有限公司,武汉 430206
Author(s):
GAO Fei1; TANG Jing2; LI Jing-gang1; ZHANG Feng1; LONG Li2; CAO De-hua2; WANG Jian2
1.Insitute of Hematology,Fujian Medical University Union Hospital, Fuzhou 350001,China; 2.Wuhan Landing Medical High-Tech Co.Ltd,Wuhan 430206,China
关键词:
人工镜检;  有核细胞;  LD BC-Ⅰ型全自动血细胞图像分析仪
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 对LD BC-Ⅰ型全自动血细胞图像分析仪的临床性能进行验证及评价。方法 收集2016年10～12月福建医科大学附属协和医院门诊EDTA-Na2抗凝血液样本202例,涂片经瑞氏染色后,分别进行人工镜检和血细胞图像分析仪扫描,比较人工镜检与自动分析仪检测效率及有核细胞分类结果的差异,进一步分析两种方法所测结果的相关性与一致性。结果 自动分析仪分析每份标本所用平均时间较人工镜检缩短3.81±2.11 min,差异具有统计学意义(t=25.626,P<0.01)。分析仪预分析原始细胞、早幼、中幼及晚幼粒细胞、异型淋巴细胞的符合率分别是71.4%,64.8%,28.8%,21.1%和71.6%。经人工复核后,五种有核细胞诊断符合率提高到87.7%,81.5%,38.1%,26.3%和86.2%。Passing-Bablok回归法显示,两种方法检测中性粒细胞、淋巴细胞、单核细胞、嗜酸性粒细胞,呈显著正相关(r=0.981,0.894,0.725,0.772,均P<0.01)。Bland-Altman分析显示,两种方法的中性粒细胞、淋巴细胞、单核细胞、嗜酸性粒细胞的符合率分别为96%,96%,91%和94%。结论 LD BC-Ⅰ型血细胞图像全自动分析仪能显著提高有核细胞的分析效率,对成熟的粒细胞、单核细胞及淋巴细胞分类结果与人工镜检一致性好,有一定的临床应用推广价值。
Abstract:
Abstract:Objective To verify and evaluate the clinical performance of LD BC-Ⅰ blood cell image automatic analyzer.MethodsA total of 202 EDTA-Na2 anticoagulant blood specimens were collected at the hospital clinic randomly between October and December 2016.After wright staining,each specimen was examined by microscopy and automatic analyzer respectively,the detection efficiency and the results of nuclear cells classification were compared between artificial microscopy and automatic analyzer,the correlation and consistency of two methods were further analyzed.Results The average time required for each specimen of the automatic analyzer was reduced by 3.81 minutes when compared with artificial microscopy,theP values was less than 0.01.LD BC-Ⅰ agreed 71.4%,64.8%,28.8%,21.1% and 71.6% respectively for pre-differentiation of the blast cells,promyelocytes,myelocyte,metamyelocytes and atypical lymphocytes.The diagnosis accordance rates of five nucleated cells above increased to 87.7%,81.5%,38.1%,26.3% and 86.2%after manual review.Passing-Bablok regression analysis found that the correlation coefficient(r)of two methods of neutrophils was 0.981,lympho kjcytes(r=0.894),monocytes(r=0.725),eosinophils(r=0.772),and there were significant correlation between two methods(all P<0.01).Bland-Altman analysis foundthat the coincidence rate of the neutrophils and lymphocytes were 96%,the monocytes were 91% and the eosinophils were 94%.Conclusion The LD BC-Ⅰ automatic blood cell image analyzer could significantly improve theanalysis efficiency of the nucleated cells.There was good consistency in the classification results of mature granulocytes,monocytes and lymphocytes between instrumental detection and artificial microscopy.The method has certain clinical value to be applied widely.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省血液医学中心建设资助项目[闽政办2017(4)号]; 国家和福建省临床重点专科建设资助项目。 作者简介:高 飞(1970-),男,硕士研究生,副主任技师,主要从事血液病实验诊断工作,E-mail:fuzhougaofei@163.com。 通讯作者:汪 键(1957-),男,博士,教授,主要从事骨髓、血液检测仪器研究,E-mail:wangjian@landing-med.com。
更新日期/Last Update: 2018-04-16