[1]陈芳芳a,张群燕b,邢 婕a,等.SLE患者外周血中性粒细胞CD64指数在鉴别并发细菌感染与疾病活动期的临床价值[J].现代检验医学杂志,2018,33(03):14-17.[doi:10.3969/j.issn.1671-7414.2018.03.005]
　CHEN Fang-fanga,ZHANG Qun-yanb,XING Jiea,et al.Clinical Value of Peripheral Blood Neutrophil CD64 Index in Identifying SLE Complicated with Bacterial Infection and Active SLE[J].Journal of Modern Laboratory Medicine,2018,33(03):14-17.[doi:10.3969/j.issn.1671-7414.2018.03.005]
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SLE患者外周血中性粒细胞CD64指数在鉴别并发细菌感染与疾病活动期的临床价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年03期
页码:
14-17
栏目:
论著
出版日期:
2018-07-17

文章信息/Info

Title:
Clinical Value of Peripheral Blood Neutrophil CD64 Index in Identifying SLE Complicated with Bacterial Infection and Active SLE
文章编号:
1671-7414(2018)03-014-04
作者:
陈芳芳a; 张群燕b; 邢 婕a; 李晓军a
南京总医院a.中心实验科; b.中西医结合科,南京 210002
Author(s):
CHEN Fang-fanga; ZHANG Qun-yanb; XING Jiea; LIXiao-juna
a.Department of Clinical Laboratory; b.Department of Integrated Traditional Chinese and Western Medicine,Nanjing General Hospital,Nanjing 210002,China
关键词:
CD64指数;  系统性红斑狼疮;  流式细胞术;  感染
分类号:
R593.241; R446.11
DOI:
10.3969/j.issn.1671-7414.2018.03.005
文献标志码:
A
摘要:
目的 探讨中性粒细胞CD64表达在鉴别系统性红斑狼疮(systemic lupus erythematosus,SLE)并发细菌感染与SLE活动期的临床价值。方法随机选取2017年1～5月期间南京总医院就诊的健康体检者30例,SLE患者34例[其中SLE疾病并发细菌感染组12例(其中并发肺炎3例,其他部位细菌感染9例),按SLE疾病活动指数(systemic lupus erythematosus disease activity-index,SLE-DAI)分为SLE活动组12例(SLE-DAI≥9分)和SLE稳定期组10例(SLE-DAI<9分)]。各组分别进行血细胞计数,C反应蛋白(CRP)和红细胞沉降率(ESR)的检测; 用流式细胞术检测外周血中性粒细胞CD64的平均荧光强度(mean fluorescence intensity,MFI),并计算出CD64指数(CD64 I)及最佳临界值。筛选出诊断SLE并发细菌感染的差异指标,绘制受试者工作特征曲线(receiver operating characteristic,ROC),对CD64 I联合其他指标判断SLE并发细菌感染的敏感度及特异度进行比较。结果 SLE并发细菌感染组CD64 I(0.47±0.40)明显高于SLE活动期组(0.17±0.13)、稳定期组(0.14±0.09)及健康对照组(0.12±0.64)(均P<0.05); ROC曲线分析结果显示:CD64 I判断SLE并发细菌感染的敏感度及特异度分别为81.8%和80.0%。结论 与3个炎性指标相比,中性粒细胞CD64 I对判断SLE并发细菌感染具有更高的准确度,有望用于SLE活动期患者与并发细菌感染者的鉴别。
Abstract:
Abstract:Objective To investigate the clinical value ofneutrophil CD64 expression in the differential diagnosis of systemic lupus erythematosus(SLE)and combined bacterial infection.Methods According to the SLE disease activity index(SLE-DAI),12 patients(SLE-DAI≥9)and 10 patients with stable SLE(SLE-DAI<9)were divided into SLE activity group.12 cases of SLE complicated bacterial infection and 30 cases of healthy control were followed-up from Nanjing General Hospital during the period from January to May 2017.Each group was tested for WBC,C-reactive protein(CRP)anderythrocyte sedimentation rate(ESR).The mean fluorescence intensity(MFI)of neutrophils CD64 was measured by flow cytometry.The CD64 index(CD64 I)and theoptimal cut off value were calculated.The sensitivity and specificity of the CD64 I combined with other indicators in the differential diagnosis of SLE infection were compared by mapping the receiver operating characteristic(ROC)curve.Results The CD64 I(0.47±0.40)in SLE combined bacterial infection group was significantly higher than that in active group(0.17±0.13),stable group(0.14±0.09)and healthy control group(0.12±0.64)(P<0.05).The sensitivity and the specificity of CD64 index was 81.8% and 80.0%,respectively.Conclusion Compared with the three inflammatory indexes,the CD64 I of neutrophils had higher accuracy in the diagnosisofSLE complicated bacterial infection,which can be used as the differential diagnosis marker both active and complicated infection SLE.

参考文献/References:

[1] Shaharir SS,Hussein H,Rajalingham S,et al.Damage in the multiethnic malaysian systemic lupus erythematosus(SLE)cohort:comparison with othercohorts worldwide[ J].PLoS One,2016,11(11):e0166270.
[2] Wang Z,Wang Y,Zhu R,et al.Long-term survival and death causes of systemic lupus erythematosus in China:a systemic review of observational Studies[J].Medicine,2015,94(17):e794.
[3] 王 芊,华 川.中性粒细胞CD64在感染诊断中的研究进展[J].临床军医杂志,2015,43(1):78-80,100. Wang Q,Hua C.The progress of neutrophil CD64 in the diagnosis of infection[J].Clinical Journal of Medical Officers,2015,43(1):78-80,100.
[4] 冯 萍,虞培娟,朱雪明.细菌感染患者外周血中性粒细胞表面CD64指数的变化[J].检验医学,2010,25(2):96-99. Feng P,Yu PJ,Zhu XM.Change of neutrophil CD64 index in the peripheral blood of patients with bacterial infection[J].Laboratory Medicine,2010,25(2):96-99.
[5] 蔡鹏威.中性粒细胞CD 64定量检测在感染性疾病检测中的应用[J].实验与检验医学,2014,32(6):719-721. Cai PW.Detection of neutrophil CD 64 in the detection of infectiousdiseases[J].Experimental and Laboratory Medicine,2014,32(6):719-721.
[6] Li Y,Lee PY,Sobel ES,et al.Increased expression of FcgammaRI/CD64 oncirculating monocytes parallels ongoing inflammation and nephritis in lupus[J].Arthritis Res Ther,2009,11(1):R6.
[7] 武建国.SLE和类风湿关节炎的新分类标准[J].临床检验杂志,2013,31(7):481-483. Wu JG.A new classification standard for SLE and rheumatoid arthritis[J].Chinese Journal of Clinical Laboratory Science,2013,31(7):481-483.
[8] Costa-Reis P,Nativ S,Isgro J,et al.Major infections in a cohort of 120 patients with juvenile-onset systemic lupus erythematosus[J].Clin Immunol,2013,149(3):442-449.
[9] Baek YW,Brokat S,Padbury JF,et al.Inter-alpha inhibitor proteins ininfants and decreased levels in neonatal sepsis[J].J Pediatr,2003,143(1):11-15.
[10] 林 阳,万岁桂.中性粒细胞表面CD64在细菌感染诊断与鉴别诊断中的应用价值[J].标记免疫分析与临床,2014,21(2):111-114. Lin Y,Wan SG.Clinical value of surface neutrophil CD64 in diagnosis and differential diagnosis of bacterial infection[J].Labeled Immunoassays and Clinical Medicine,2014,21(2):111-114.
[11] 闫佩毅,张 骥,邹玉涵,等.流式细胞术检测CD64指数及其在诊断细菌感染中的应用[J].检验医学,2017,32(6):514-519. Yan PY,Zhang J,Zou YH,et al.Determination of CD64 index by flow cytometry in the diagnosis of bacterial infection[J].Laboratory Medicine,2017,32(6):514-519.
[12] 陈 江,熊永红,杨学强,等.CD64感染指数与细菌感染相关性研究[J].检验医学与临床,2017,14(7):979-981. Chen J,Xiong YH,Yang XQ,et al.Research on correlation between CD64 infectious index and bacterial infection[J].Lab Med Clin,2017,14(7):979-981.
[13] 张 晶,张 露,刘园园.中性粒细胞CD64指数在血流和局部细菌感染中的诊断价值[J].国际检验医学杂志,2017,38(19):2760-2762. Zhang J,Zhang L,Liu YY.The diagnostic value of neutrophil CD64 index inblood flow and local bacterial infection[J].Int J Lab Med,2017,38(19):2760-2762.
[14] 卢小岚,蔡嘉镜,蔡艳娟,等.外周血CD64 index对细菌感染性疾病的诊断价值[J].现代检验医学杂志,2014,29(1):65-68. Lu XL,Cai JJ,Cai YJ,et al.Value of CD64 index in diagnosis of bacterial infection[J].J Mod Lab Med,2014,29(1):65-68.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(No 81470071),国家临床重点专科建设项目(No 2014ZDZK003-1),江苏省临床医学科技专项(重点病种的规范化诊疗研究)(No BL2014072),军区医药卫生科研重大专项(No 14ZX17)。 作者简介:陈芳芳(1984-),女,博士,主管技师,主要从事免疫检验工作,E-mail: longysh@163.com。 通讯作者:李晓军(1962-),女,博士,主任技师,主要研究方向:临床检验诊断学,E-mail:xiaojunli62@126.com。
更新日期/Last Update: 2018-04-16