[1]李 君,侯金友,张 慧,等.临床实验室早期诊断ABO-HDN三种增强试验应用比较研究[J].现代检验医学杂志,2018,33(03):133-135.[doi:10.3969/j.issn.1671-7414.2018.03.034]
　LI Jun,HOU Jin-you,ZHANG Hui,et al.Comparative Study on the Three Enhanced Tests for Early Diagnosis of ABO-HDN in Clinical Laboratory[J].Journal of Modern Laboratory Medicine,2018,33(03):133-135.[doi:10.3969/j.issn.1671-7414.2018.03.034]
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临床实验室早期诊断ABO-HDN三种增强试验应用比较研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年03期
页码:
133-135
栏目:
研究简报·实验技术
出版日期:
2018-07-17

文章信息/Info

Title:
Comparative Study on the Three Enhanced Tests for Early Diagnosis of ABO-HDN in Clinical Laboratory
文章编号:
1671-7414(2018)03-133-03
作者:
李 君; 侯金友; 张 慧; 卢焱坤; 邹红蕊; 张晓磊; 张秀梅
开滦总医院输血科,河北唐山 063000
Author(s):
LI Jun; HOU Jin-you; ZHANG Hui; LU Yan-kun;  ZOU Hong-rui; ZHANG Xiao-lei; ZHANG Xiu-mei
Department of Blood Transfusion,Kailuan General Hospital,HebeiTangshan 063000,China
关键词:
增强试验;  ABO-新生儿溶血病;  实验室早期诊断
分类号:
R457.1
DOI:
10.3969/j.issn.1671-7414.2018.03.034
文献标志码:
A
摘要:
目的 探讨《HDN产后免疫血液学试验推荐方案》中增强试验在临床实验室早期诊断ABO系统新生儿溶血病(HDN)中的应用效果。方法 可疑ABO-HDN患儿274例,检测母亲ABO,RhD血型和IgG抗-A/B效价及患儿ABO,RhD血型和三项溶血试验,采用经典抗球蛋白试验(对照组)和《HDN产后免疫血液学试验推荐方案》中的三种增强试验分别是酶处理细胞增强试验(试验组1)、添加低离子强度(LISS)溶液(试验组2)、添加聚乙二醇(PEG)溶液(试验组3)检测游离抗体和放散液抗体,比较四种方法的临床诊断效果。结果 274例患儿确诊ABO-HDN 161例(58.8%),其中A型87例(54.0%),B型74例(46.0%),不同血型患儿HDN患病率差异无统计学意义(χ2 =0.089,P>0.05)。161例ABO-HDN患儿中直抗阳性25例(15.5%)。对照组放散试验漏检率为5.8%(16/274)。试验组游离试验和放散试验检出率均高于对照组,其中试验组1检出率最高,试验组3次之,试验组2低于1,3组(χ2 游离=46.438,χ2放散 =37.534,均P<0.05)。HDN发病例数和总胆红素水平随着酶处理细胞增强试验放散液抗体效价增高而增加(χ2 =18.931,F=12.624,P<0.05)。结论 酶处理细胞增强试验检出效果最佳,能为实验室早期诊断ABO-HDN提供可靠的依据。
Abstract:
Abstract:Objective To discuss the application of enhanced tests recommended in the 《HDN postpartum immunity hematology test recommendation》 for early diagnosis of ABO-HDN in clinical laboratory.MethodsCollected 274 cases of children with suspected ABO-HDN,tested ABO,RhD type and anti-A/B IgG titer of their mother,and test children ABO,RhD type and three hemolysis test of children used classical antiglobulin test(control group)and three kinds of enhancing experiment respectively enzymatic processing enhancement test(group 1),add low ionic strength solution(LISS)(group 2),adding polyethylene glycol(PEG)solution(group 3)introduced in the 《HDN postpartum immunity hematology test recommendation》to detect free antibodies andantibodies of elution liquid.Then compared clinical diagnosis effect of the four methods.Results 161 cases were diagnosed ABO - HDN inthe 274 cases(58.8%),type A 87 cases(54.0%),type B 74 cases(46.0%),andthere was no statistical significance between different ABO blood group of HDN(χ2=0.089,P>0.05).25 cases(15.5%)were positive in direct antiglobulin test.The missing rate of the control group in tossing test was 5.8%(16/274).The positive rate of group 1,2,3 in free test and tossing test were all higher than the control group.Experimental group 1 had the highest detection rate,while group 2 was less than group 1 and group 3(χ2 游离=46.438,χ2 放散=37.534,all P<0.05).The incidence of HDN and the total bilirubin level was increasing with the increase of antibody titer detected using enzymatic processing enhancement test(χ2=18.931,F=12.624,P<0.05).Conclusion Enzymatic processing enhancement test was optimal.It could provide reliable reference for early diagnosis of ABO-HDN in clinical laboratory.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:李 君(1985-)女,硕士,主管技师,主要从事输血前免疫学实验工作,E-mail:278800026@qq.com。
更新日期/Last Update: 2018-04-16