[1]刘玉林,黄晓楠,赵琪林,等.RI与ANG相互作用对BALB/C裸鼠人膀胱癌移植瘤生长转移及PI3K/AKT/mTOR信号通路的影响[J].现代检验医学杂志,2018,33(04):34-38.[doi:10.3969/j.issn.1671-7414.2018.04.009]
 LIU Yu-lin,HUANG Xiao-nan,ZHAO Qi-lin,et al.Effects of Interaction of Ribounclease Inhibitor and Human Angiogenin on the Growth of Transplanted BALB/C Bladder Cancer and the Expression of PI3K/AKT/Mtor[J].Journal of Modern Laboratory Medicine,2018,33(04):34-38.[doi:10.3969/j.issn.1671-7414.2018.04.009]
点击复制

RI与ANG相互作用对BALB/C裸鼠人膀胱癌移植瘤生长转移及PI3K/AKT/mTOR信号通路的影响()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
34-38
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Effects of Interaction of Ribounclease Inhibitor and Human Angiogenin on the Growth of Transplanted BALB/C Bladder Cancer and the Expression of PI3K/AKT/Mtor
文章编号:
1671-7414(2018)04-034-05
作者:
刘玉林1黄晓楠1赵琪林1胥国强1赵明才1陈俊霞2
1.遂宁市中心医院检验科,四川遂宁 629000; 2.重庆医科大学细胞生物学与遗传学教研室,重庆 400016
Author(s):
LIU Yu-lin1HUANG Xiao-nan1ZHAO Qi-lin1XUGuo-qiang1ZHAO Ming-cai1 CHEN Jun-xia2
1.Department of Clinical Laboratory, Central Hospital of Suining,Sichuan Suining 629000,China; 2.Departmentof Cell Biology and Genetics,Chongqing Medical University,Chongqing 400016,China
关键词:
核糖核酸酶抑制因子 血管生成素 移植瘤 信号通路
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2018.04.009
文献标志码:
A
摘要:
目的 探讨核糖核酸酶抑制因子(RI)与血管生成素(ANG)相互作用对BALB/C裸鼠人膀胱癌(cloladder cancer)移植瘤的生长转移及对PI3K/AKT/mTOR信号通路的影响。方法 构建BALB/C裸鼠人膀胱癌移植瘤模型,通过HE染色、免疫组织化学染色、免疫荧光和免疫印迹法检测相关通路蛋白的表达情况。根据不同的组合分为:BIU-87+ANG,BIU-87+RI,BIU-87+ANG+RI和BIU-87+空质粒组,每组接种6只BALB/C裸鼠。结果 接种后一周左右皮下有实体瘤生成,30天后处死BALB/C裸鼠,取出肿瘤并称取其重量。实验组BIU-87+RI和BIU-87+ANG+RI组比对照组的肿瘤重量低,差异具有统计学意义(P<0.05)。肺HE染色观察显示实验组肺部转移结节明显比对照组少,BIU87+ANG组中p-mTOR,p-PI3K,p-Akt和p-GSK3β表达升高,BIU87+RI组和BIU-87+ANG+RI组表达下降。结论 RI与ANG相互作用抑制BALB/C裸鼠人膀胱癌移植瘤的生长转移,同时抑制PI3K/AKT/mTOR信号通路中磷酸化蛋白的水平。
Abstract:
Abstract:Objective To research the interaction between ribonuclease inhibitor(RI)and angiogenin(ANG)and its effect on the growth oftransplanted BALB/C bladder cancer and the expression of PI3K/AKT/mTOR.Methods Bladder cancer nude mouse transplantation tumor model wasconstructed.Used HE staining and immunohistochemical staining and immunofluorescence and western blot method to detect the related pathway of protein expression.The groups were named as BIU-87+ANG,BIU-87+RI,BIU-87+ANG+RI and BIU-87+empty vector respectively.Results About a week after inoculation subcutaneous solid tumor formation,put to death in mice after 5 weeks,removed tumors and took its weight.Experimental tumor of average weight increased significantly than the control group on average weight,with statistical significance(P<0.05).Lung HE staining showed experimental lung metastasis nodule significantly less than the control group.The p-mTOR,p-PI3K,p-Akt and p-GSK3β of protein expression had increased in BIU87+ANG group,but theyhad decreased in BIU87+RI group and BIU-87+ANG+RI group.Conclusion Interaction between RI and ANG inhibit the growth of bladder cancer transplantation tumor metastasis and PI3K/AKT/mTOR signaling pathway in phosphorylation protein levels.

参考文献/References:

[1] Dalbagni G.Bladder cancer:estaging TUR reduces recurrence and progression risk[J].Nat Rev Urol,2010,7(12):649-650.
[2] Dickson KA,Kang DK,Kwon YS,et al.Ribonuclease inhibitor regulates neovascularization by human angiogenin[J].Biochemistry,2009,48(18):3804-3806.
[3] Acharya KR,Shapiro R,Allen SC,et al.Crystal structureof human angiogenin reveals the structural basis for its functional divergencefrom ribonuclease[J].Proc Natl Acad Sci USA,1994,91(8):2915-2919.
[4] Yoshioka N,Wang L,Kishimoto K,et al.A therapeutic target for prostatecancer based on angiogenin-stimulated angiogenesis and cancer cell proliferation[J].Proc Natl Acad Sci USA,2006,103(39):14519-14524.
[5] 潘湘阳,李红彦,姚 雪,等.核糖核酸酶抑制因子过表达抑制B16细胞的侵袭和转移[J].第三军医大学学报,2012,34(4):284-289. Pan XY,Li HY,Yao X,et al.Over-expression of human ribonuclease inhibitor suppresses invasion and migration in B16 melanoma cells[J].J Third Mil Med Univ,2012,34(4):284-289.
[6] 刘玉林,庄 翔,陈俊霞.血管生成素及其突变体与核糖核酸酶抑制因子蛋白的相互作用[J].基础医学与临床,2015,35(3):313-317. Liu YL,Zhuang X,Chen JX.Ribonuclease inhibitor interacts with angiogenin and its mutant[J].Basic&Clinical Medicine,2015,35(3):313-317.
[7] 李 林,舒 静,刘玉林,等.核糖核酸酶抑制因子与血管生成素的相互作用[J].第三军医大学学报,2014,36(14):1454-1459. Li L,Shu J,Liu YL,et al.Protein-protein interaction between ribonuclease inhibitor and angiogenin in mammalian cells[J].J Third Mil Med Univ,2014,36(14):1454-1459.
[8] Stumpp MT,Forrer P,Binz HK,et al.Designing repeat proteins:modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family[J].J Mol Biol,2003,332(12):471-487.
[9] Pan X,Xiong D,Yao X,et al.Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells[J].Int J Biochem Cell Biol,2012,44(6):998-1008.
[10] Chen J,Ouyang X,Gao J,et al.Knockdown of ribonuclease inhibitor expression with siRNA in non-invasive bladder cancer cell line BIU-87 promotes growth and metastasis potentials[J].Mol Cell Biochem,2011,349(1/2):83-95.
[11] Li S,Ibaragi S,Hu GF,et al.Angiogenin as a molecular target for thetreatment of prostate cancer[J].Curr Cancer Ther Rev,2011,7(2):83-90.
[12] Li L,Pan XY,Shu J,et al.Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repressionof angiogenin and ILK/PI3K/AKT signaling pathway[J].Biochimie,2014,103(1):89-100.
[13] 涂业桃,舒 静,田文林,等.下调血管生成素基因表达对膀胱癌移植瘤生长及p-AKT,p-GSK3β,p-mTOR表达的影响[J].广东医学,2015,36(4):501-504. Tu YT,Shu J,Tian WL,et al.Effects of down-regulation of human angiogenin on the growth of transplanted bladder cancer and the expression of p-Akt,p-GSK3β and p-mTOR[J].Guangdong Medical Journal,2015,36(4):501-504.
[14] Wang SS,Chen YH,Chen N,et al.Hydrogen sulfide promotes autophagy ofhepatocellular carcinoma cells through the PI3K/Akt/mTOR signaling pathway[J].Cell Death Dis,2017,8(3):e2688.

备注/Memo

备注/Memo:
作者简介:刘玉林(1988-),男,硕士,检验师,主要从事肿瘤分子生物学研究,E-mail:liuyulin615200@126.com。 通讯作者:陈俊霞,女,教授,E-mail:chjunxia@126.com。
更新日期/Last Update: 2018-08-16